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Product Listing

PCR and qPCR

Amplification of DNA fragments by DNA polymerase to produce millions of copies

PCR (polymerase chain reaction) utilizes the ability of naturally occurring DNA polymerases to extend short single-stranded primers that hybridize to the target template DNA. Despite its wide utility, most commercially available PCR reagents are remarkably similar, with any performance difference among them being attributed to differences in buffer formulation or enzyme concentration. These reagents contain wild-type enzymes, isolated from nature without modification. Roche offers the KAPA PCR reagents containing DNA polymerase and other enzymes selected through our Directed Evolution Technology to deliver high-quality results for specific PCR applications.

Roche offers the KAPA PCR reagents optimally suited for a variety of applications. KAPA HiFi PCR Kits , which use KAPA HiFi DNA Polymerase engineered for high fidelity and robustness, are used specifically for library amplification.

Available products by application

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Available products by application

Application Product Features and Benefits
Cloning KAPA Long Range PCR Kits
  • Amplification of inserts up to 15 kb with high sensitivity and an error rate 4X lower than Taq DNA polymerase. Compatible with TA cloning
Colony PCR
 KAPA2G Robust PCR Kits
  • Improved tolerance to PCR inhibitors results in higher success rates and streamlined workflows with common E. coli and S. cerevisiae strains*
Crude Sample PCR

KAPA2G Robust PCR Kits

KAPA3G Plant PCR Kits

KAPA Mouse Genotyping Kits
  • Improved resistance to many inhibitors commonly found in crude samples—such as denatured protein, salts and metabolites—results in higher PCR success rates and more streamlined workflows*
  • Improved resistance to inhibitors co-purified in plant DNA extracts, and amplification directly from plant tissues enable higher-throughput workflows and eliminate the need for expensive and  time-consuming purification*
  • Increased throughput and decreased turnaround time*
  • Better performance compared to other available crude extraction methods *
Direct Plant PCR
 KAPA3G Plant PCR Kits
  • Improved resistance to inhibitors co-purified in plant DNA extracts, and amplification directly from plant tissues enable higher-throughput workflows and eliminate the need for expensive and time-consuming purification*
Fast PCR KAPA2G Fast PCR Kits
  • Higher specific activity and processivity significantly reduces PCR cycling times by 50% without a loss in performance*
GC-rich PCR KAPA2G Robust PCR Kits
  • Robust performance, shorter cycling times, streamlined workflows and higher success rates across a wide variety of template types and amplicons*
Genotyping

KAPA2G Robust PCR Kits

KAPA2G Fast PCR Kits

KAPA Taq PCR Kits

KAPA Mouse Genotyping Kits
  • Robust performance, shorter cycling times, streamlined workflows and higher success rates across a wide variety of template types and amplicons*
  • Reduced PCR cycling times and improved success rates streamline workflows for routine genotyping*
  • High quality and optimal formulation for routine genotyping applications
  • Increased throughput and decreased turnaround time*
  • Better performance compared to other available crude extraction methods*
High-fidelity PCR KAPA Long Range PCR Kits
  • Recommended for the amplification of longer targets up 15 kb with improved sensitivity and an error rate 4X lower than Taq DNA polymerase
Long-range PCR KAPA Long Range PCR Kits
  • Recommended for the amplification of longer targets up 15 kb with improved sensitivity and an error rate 4X lower than Taq DNA polymerase*
Multiplex PCR KAPA2G Fast Multiplex Kits
  • Increased specific activity and processivity, formulated for highly sensitive and uniform multiplex amplification*
Single-protocol PCR

KAPA2G Robust PCR Kits

KAPA2G Fast PCR Kits
  • Robust performance across a wide range of GC- and AT-rich templates streamlines workflows while reducing turnaround times. Recommended for end-point applications using gel electrophoresis
  • Broad coverage of both AT- and GC-rich targets consolidates protocols and reduces cycling times. Recommended for higher sensitivity detection such as capillary electrophoresis and Sanger sequencing workflows
*Data on file.
Overview

Quantitative real-time PCR (qPCR) enables the detection and quantification of PCR products in real time and is used widely in gene expression studies due to its high sensitivity, reproducibility and wide dynamic range. The technique uses double-stranded DNA (dsDNA)-binding dyes or target-specific fluorescently labeled primers and probes that hybridize with the target sequence to measure the relative amount of DNA. Most commercially available qPCR reagents contain wild-type, unmodified DNA polymerase, but these enzymes were never intended as tools for use in molecular biology.  The performance differences among commercial qPCR kits are the result of differences in factors like buffer formulation and/or enzyme concentration.

Roche offers KAPA qPCR reagents containing DNA polymerase and other novel enzymes specifically selected through our directed evolution technology for qPCR applications. These confer significant improvements to reaction efficiency, sensitivity, speed, and signal-to-noise ratio.

Available products by application

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Available products by application

Application Product Features and Benefits
Gene Expression

KAPA SYBR FAST qPCR Kits

KAPA SYBR FAST One-Step qRT-PCR Kits

KAPA PROBE FAST qPCR Kits

KAPA PROBE FORCE qPCR Kits
  • Higher sensitivity and improved reaction efficiency across a broad range of amplicon sizes and GC contents*
  • Reduced experimental variation and contamination with a convenient one-step qRT-PCR protocol*
  • Optimized for versatility and speed, providing highly reproducible results for gene expression assays
  • Direct qPCR from crude blood, tissue, and plant extracts.
  • Sample-to-Cq (workflows in <1 hour*)
  • High efficiency for accurate, reproducible, and sensitive results
Genotyping

KAPA SYBR FAST qPCR Kits

KAPA PROBE FAST qPCR Kits

KAPA PROBE FORCE qPCR Kits
  • Higher sensitivity and improved reaction efficiency across a broad range of amplicon sizes and GC contents*
  • Optimized for versatility and speed, compatible with all probe-based SNP detection applications
  • Engineered to maximize differences in melting behavior between sequence variants resulting in accurate SNP genotyping with maximum sensitivity and speed
  • Direct qPCR from crude blood, tissue, and plant extracts.
  • Sample-to-Cq (workflows in <1 hour)
  • High efficiency for accurate, reproducible, and sensitive results
Food and Water Safety
 KAPA PROBE FORCE qPCR Kits
  • Direct qPCR from crude blood, tissue, and plant extracts
  • Sample-to-Cq (workflows in <1 hour*)
  • High efficiency for accurate, reproducible, and sensitive results
*Data on file.

Articles & Publications

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