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The Kapa Taq DNA polymerase enzyme is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. Kapa Taq and Kapa Taq HotStart DNA polymerase enzymes have 5′-3′ polymerase and 5′-3′ exonuclease activities, but no 3′ to 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated.
View data showing superior PCR performance with the amplification of CCR5 gene fragment using KAPA Taq PCR Kit compared to competitor kits.
See data showing higher sensitivity and less primer dimers using Kapa HotStart enzyme compared to KAPA Taq PCR Kit from mycoplasma DNA fragment amplification.
Figure 1. A 500 bp fragment of the CCR5 gene was amplified using 100 ng, 10 ng, 1 ng or 100 pg of human genomic DNA as template. Kapa Taq HotStart enzyme exhibits improved sensitivity, specificity, and yield when compared with competitive HotStart products. All reactions were performed using the manufacturer-recommended protocols.
Figure 2. A 270 bp amplicon was amplified from mycoplasma DNA with Kapa Taq or Kapa Taq HotStart polymerase. Sensitivity was tested using a 10X template dilution series starting with 1 ng of DNA. Higher sensitivity and less primer dimers are observed when using Kapa Taq HotStart polymerase.
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Kits can be stored for up to 18 months at -20˚C.
Kits include KAPA Taq (or KAPA Taq HotStart) DNA Polymerase (5 U/µL), KAPA Taq Buffer, and MgCl2 (25 mM). Kits are available with and without dNTP Mix (10 mM each). The following reaction buffers are available:
KAPA Taq HotStart DNA Polymerase is also sold separately.
ReadyMix formulations (2X) of KAPA Taq DNA Polymerase are also available. These contain DNA Polymerase, reaction buffer (with and without dye), dNTPs and MgCl2 (at a final concentration of 1.5 mM).
| Components |
|---|
| KAPA Taq Standard or HotStart DNA Polymerase 5 U/µL |
| 10X KAPA Taq Buffer A |
| 10X KAPA Taq Buffer B |
| 10X KAPA Buffer with loading dye (optional) |
| 5X KAPA Taq HotStart Buffer (HotStart kits only) |
| MgCl2 (25 mM) |
| dNTP Mix (10 mM each; optional) |
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| KAPA Code |
Roche Cat. No |
Description |
Kit Size |
How to buy |
|---|---|---|---|---|
| BK1000 |
07958447001 |
Standard kit | 2500 U |
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| BK1002 |
07958463001 |
Standard kit | 5000 U |
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| KK1014 |
07958579001 |
Standard kit | 250 U |
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| KK1015 |
07958587001 |
Standard kit | 500 U |
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| KK1016 |
07958595001 |
With dNTPs | 500 U |
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| KK1020 |
07958617001 |
With dye | 250 U |
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| KK1022 |
07958633001 |
With dye | 500 U |
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| KK1508 |
07958765001 |
HotStart PCR Kit | 250 U |
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| KK1509 |
07958773001 |
HotStart PCR Kit, with dNTPs | 250 U |
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| KK1510 |
07958781001 |
HotStart PCR Kit | 500 U |
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| KK1511 |
07958790001 |
HotStart PCR Kit, with dNTPs | 500 U |
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| KK1513 |
07958811001 |
HotStart PCR Kit | 2500 U |
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| KAPA Code |
Roche Cat. No |
Description |
Kit Size |
How to buy |
|---|---|---|---|---|
| KK1006 |
07958536001 |
ReadyMix, 250 x 50 µL reactions | 6.25 mL |
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| KK1024 |
07958650001 |
ReadyMix with dye, 250 x 50 µL reactions | 6.25 mL |
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