KAPA Taq PCR Kits

For Research Use Only. Not for use in diagnostic procedures.

Overview

The Kapa Taq DNA polymerase enzyme is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. Kapa Taq and Kapa Taq HotStart DNA polymerase enzymes have 5′-3′ polymerase and 5′-3′ exonuclease activities, but no 3′ to 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 10⁵ nucleotides incorporated.

Features and Benefits of KAPA Taq PCR Kits

Hot-start formulation – combined with a proprietary antibody that inactivates the enzyme until the first denaturation step
Elimination of spurious amplification products and increase in reaction efficiency and sensitivity
Supplied in a 2X ReadyMix format containing all the components required for PCR except primers and template
Also available with loading dye reaction buffer, allowing direct loading of PCR products onto agarose gels with no extra steps for adding loading/tracking dye

Product highlights

Improved sensitivity, specificity and yield compared to other available kits

Novel buffer formulation, facilitating specific primer annealing, leading to higher yield of specific product

View data showing superior PCR performance with the amplification of CCR5 gene fragment using KAPA Taq PCR Kit compared to competitor kits.

See data showing higher sensitivity and less primer dimers using Kapa HotStart enzyme compared to KAPA Taq PCR Kit from mycoplasma DNA fragment amplification.

Figure 1. A 500 bp fragment of the CCR5 gene was amplified using 100 ng, 10 ng, 1 ng or 100 pg of human genomic DNA as template. Kapa Taq HotStart enzyme exhibits improved sensitivity, specificity, and yield when compared with competitive HotStart products. All reactions were performed using the manufacturer-recommended protocols.

Figure 2. A 270 bp amplicon was amplified from mycoplasma DNA with Kapa Taq or Kapa Taq HotStart polymerase. Sensitivity was tested using a 10X template dilution series starting with 1 ng of DNA. Higher sensitivity and less primer dimers are observed when using Kapa Taq HotStart polymerase.

For purchasing or general questions

For online ordering

Specifications

Starting Material

DNA, Plasmid DNA, cDNA
Input Amount

≤250 ng for genomic DNA; ≤25 ng for less complex DNA

Kits can be stored for up to 18 months at -20˚C.

Kits include KAPA Taq (or KAPA Taq HotStart) DNA Polymerase (5 U/µL), KAPA Taq Buffer, and MgCl₂ (25 mM). Kits are available with and without dNTP Mix (10 mM each). The following reaction buffers are available:

KAPA Taq Buffer A and B (10X, with 1.5 mM MgCl₂ at 1X), supplied with KAPA Taq PCR Kits only
KAPA Taq Buffer with dye (10X, with 1.5 mM MgCl₂ at 1X), supplied with KAPA Taq PCR Kits with dye only
KAPA Taq HotStart Buffer (5X, Mg-free), supplied with KAPA Taq HotStart PCR Kits only

KAPA Taq HotStart DNA Polymerase is also sold separately.

ReadyMix formulations (2X) of KAPA Taq DNA Polymerase are also available. These contain DNA Polymerase, reaction buffer (with and without dye), dNTPs and MgCl₂ (at a final concentration of 1.5 mM).

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Components
KAPA Taq Standard or HotStart DNA Polymerase 5 U/µL
10X KAPA Taq Buffer A
10X KAPA Taq Buffer B
10X KAPA Buffer with loading dye (optional)
5X KAPA Taq HotStart Buffer (HotStart kits only)
MgCl₂(25 mM)
dNTP Mix (10 mM each; optional)

For purchasing or general questions

For online ordering

Component

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KAPA Code	Roche Cat. No	Description	Kit Size	How to buy
BK1000	07958447001	Standard kit	2500 U	Login for pricing
BK1002	07958463001	Standard kit	5000 U	Login for pricing
KK1014	07958579001	Standard kit	250 U	Login for pricing
KK1015	07958587001	Standard kit	500 U	Login for pricing
KK1016	07958595001	With dNTPs	500 U	Login for pricing
KK1020	07958617001	With dye	250 U	Login for pricing
KK1022	07958633001	With dye	500 U	Login for pricing
KK1508	07958765001	HotStart PCR Kit	250 U	Login for pricing
KK1509	07958773001	HotStart PCR Kit, with dNTPs	250 U	Login for pricing
KK1510	07958781001	HotStart PCR Kit	500 U	Login for pricing
KK1511	07958790001	HotStart PCR Kit, with dNTPs	500 U	Login for pricing
KK1513	07958811001	HotStart PCR Kit	2500 U	Login for pricing

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KAPA Code	Roche Cat. No	Description	Kit Size	How to buy
KK1006	07958536001	ReadyMix, 250 x 50 µL reactions	6.25 mL	Login for pricing
KK1024	07958650001	ReadyMix with dye, 250 x 50 µL reactions	6.25 mL	Login for pricing

For purchasing or general questions

For online ordering

KAPA Taq PCR Kits

Overview

Features and Benefits of KAPA Taq PCR Kits

Resources

Product highlights

Specifications

Contents

Component