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KAPA PROBE FAST qPCR Kits

For Research Use Only. Not for use in diagnostic procedures.

KAPA qPCR

Overview

KAPA PROBE FAST qPCR Kits provide fast and reproducible results for all probe-based qPCR applications. Kits contain a ready-to-use master mix for highly sensitive and accurate real-time PCR using sequence-specific fluorogenic probe chemistries, including hydrolysis probes (e.g. TaqMan), FRET probes, and displacement probes (e.g. molecular beacons).

Benefits of KAPA PROBE FAST qPCR Kit

  • Fast and reproducible results across several probe chemistries
  • Compatibility with different qPCR protocols

Product highlights

Accurate discrimination between alleles

  • Exceptional differentiation of heterozygous and homozygous alleles*
  • Discrete clusters in SNP genotyping assays

View genotyping data showing discrete allele clusters using KAPA PROBE FAST qPCR Kits.

Excellent reproducibility and efficiency

  • Fast, high performance five-colored multiplex PCR
  • Similar abundance levels achieved with low copy number
  • Broad dynamic range of up to 10 orders of magnitude

View performance data for KAPA PROBE FAST qPCR Kits from multiplex qPCR amplifications of five amplicons.

Increased flexibility

  • Compatible with both standard and fast cycling protocols
  • Retain accurate quantification

View data showing comparable qPCR performance with standard or fast protocol using KAPA PROBE FAST qPCR Kits.

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*Data on file.

Specifications

  • Starting Material

    DNA, plasmid DNA, cDNA

Kits can be stored for up to 12 months at -20˚C.

Kits include KAPA PROBE FAST qPCR Master Mix (2X), which contains KAPA Taq HotStart DNA Polymerase, reaction buffer, dNTPs, and MgCl2 at a final concentration of 5 mM. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes ROX High and ROX Low (both at 50X) separately.

Components

 

 

   Kit code  2X KAPA PROBE qPCR Master Mix(Universal) 50X KAPA PROBE ROX High 50X KAPA PROBE ROX Low 2X KAPA PROBE qPCR Master Mix (with equipment-specific dye) 2X KAPA PROBE qPCR Master Mix (ROX Low) 10 mM dUTP
Universal KK4701 X X X      
KK4702            
KK4703            
KK4715            
ABI Prism KK4705       X    
KK4706             
KK4707            
Bio-Rad iCycler KK4709       X    
KK4710            
KK4711            
ROX Low KK4716         X  
KK4717            
KK4718            
KK4719            

For purchasing or general questions

For online ordering

Kits include reagents for RNA fragmentation, cDNA synthesis, and library preparation. Kits with reagents for mRNA capture are also available.

KAPA Code
Roche Cat. No
Description
Kit Size
How to buy
KK4701
07959800001
100 x 20 µL reactions (Universal) 1 mL
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KK4702
07959818001
500 x 20 µL reactions (Universal) 5 mL
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KK4703
07959826001
1000 x 20 µL reactions (Universal) 10 mL
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KK4705
07959842001
100 x 20 µL reactions, ROX (ABI Prism®) 1 mL
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KK4706
07959869001
500 x 20 µL reactions, ROX (ABI Prism) 5 mL
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KK4707
07959877001
1000 x 20 µL reactions, ROX (ABI Prism) 10 mL
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KK4709
07959893001
100 x 20 µL reactions, with fluorescein (Bio-Rad® iCycler™) 1 mL
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KK4710
07959907001
500 x 20 µL reactions, with fluorescein (Bio-Rad iCycler) 5 mL
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KK4711
07959915001
1000 x 20 µL reactions, with fluorescein (Bio-Rad iCycler) 10 mL
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KK4715
07959931001
5000 x 20 µL reactions (Universal) 50 mL
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KK4716
07959940001
100 x 20 µL reactions (ROX Low) 1 mL
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KK4717
07959958001
500 x 20 µL reactions (ROX Low) 5 mL
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KK4718
07959966001
1000 x 20 µL reactions (ROX Low) 10 mL
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For purchasing or general questions

For online ordering