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KAPA Mouse Genotyping Kits

For Research Use Only. Not for use in diagnostic procedures.

KAPA Mouse Genotyping Kits

Overview

KAPA Mouse Genotyping Kits include KAPA Express Extract, a novel thermostable protease and buffer system that allows for the extraction of PCR-ready DNA from mouse tissue in as little as 15 minutes. The kit also includes KAPA2G Fast Genotyping Mix with dye containing a DNA polymerase engineered via our directed evolution technology for high processivity and extreme speed. The combination of KAPA Express Extract and KAPA2G Fast Genotyping Mix allows for the reliable extraction and amplification of DNA fragments from mouse tissue in as little as 1 hour compared to ≥1 day with conventional protocols.

 

Benefits of KAPA Mouse Genotyping Kits

  • Extraction and amplification of mouse tissue DNA in 1 hour
  • Better performance than other extraction methods*

Product highlights

Increased throughput, turnaround time and reliability

  • PCR-ready DNA generated in as little as 15 minutes
  • Minimal handling, thereby reducing the risk of sample loss or contamination
  • Sufficient template for multiple assays; easily scaled to handle samples in a 96-well format
  • Fast and reliable amplification of DNA fragments across a wide range of amplicon lengths and GC contents

View comparative data showing significantly reduced extraction and amplification times and comparable PCR performance using KAPA Mouse Genotyping Kits compared to using wild type Taq and Proteinase K or alkaline lysis.

 

Superior PCR performance compared to crude extraction methods and other commercial kits

  • Better turnaround times
  • Increased specificity
  • Decreased cost*

View data showing comparable or better PCR performance, reduced cost per PCR reaction, and  better sensitivity, yield and specificity in multiplex PCR using KAPA Mouse Genotyping Kits compared to other commercially available kits.

 

For purchasing or general questions

For online ordering

*Data on file.

Specifications

  • Starting Material

    Mouse tail, ear, or toe tissue

  • Input Amount

    ~2 mm3 fragment or 2 mm2 punch

Kits can be stored for up to 18 months at -20°C.

KAPA Mouse Gentoyping Kits include KAPA Express Extract Enzyme (1 U/µL), KAPA Express Extract Buffer (10X), and KAPA2G Fast (or Fast HotStart) Genotyping Mix (2X), which contains DNA Polymerase, reaction buffer with loading dye, dNTPs, and MgCl2 (at a final concentration of 1.5 mM). ReadyMixes for PCR are also sold separately.

Contents

Components
2X KAPA2G Fast Standard or HotStart Genotyping Mix with dye
KAPA Express Extract (1 U/µL)
10X KAPA Express Extract Buffer

For purchasing or general questions

For online ordering

Ordering

KAPA Mouse Gentoyping Kits include KAPA Express Extract Enzyme (1 U/µL), KAPA Express Extract Buffer (10X), and KAPA2G Fast (or Fast HotStart) Genotyping Mix (2X), which contains DNA Polymerase, reaction buffer with loading dye, dNTPs, and MgCl2 (at a final concentration of 1.5 mM). ReadyMixes for PCR are also sold separately.

KAPA Code
Roche Cat. No
Description
Kit Size
How to buy
KK5121
07960921001
Non-HotStart PCR, without extraction 500 x 25 µL reactions
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KK5621
07961316001
HotStart PCR, without extraction 500 x 25 µL reactions
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KK7302
07961766001
Non-HotStart PCR 500 reactions
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KK7352
07961804001
Standard kit 500 reactions
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For purchasing or general questions

For online ordering