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KAPA Mouse Genotyping Kits include KAPA Express Extract, a novel thermostable protease and buffer system that allows for the extraction of PCR-ready DNA from mouse tissue in as little as 15 minutes. The kit also includes KAPA2G Fast Genotyping Mix with dye containing a DNA polymerase engineered via our directed evolution technology for high processivity and extreme speed. The combination of KAPA Express Extract and KAPA2G Fast Genotyping Mix allows for the reliable extraction and amplification of DNA fragments from mouse tissue in as little as 1 hour compared to ≥1 day with conventional protocols.
View comparative data showing significantly reduced extraction and amplification times and comparable PCR performance using KAPA Mouse Genotyping Kits compared to using wild type Taq and Proteinase K or alkaline lysis.
Figure 1. The total time required for DNA extraction and amplification was compared using the KAPA Mouse Genotyping Kit, Proteinase K and wild-type Taq, and alkaline lysis and wild-type Taq.
Figure 2. Results for five amplicons (312 –915 bp) generated with the KAPA Mouse Genotyping Kit (1) were compared to those obtained with two commonly used methods (2 and 3). With the KAPA Mouse Genotyping Kit, DNA lysates were prepared from mouse tails with the rapid (15 minutes), single-tube KAPA Express Extract system. Amplification with the KAPA2G Fast HotStart Genotyping Mix with dye was completed in 45 minutes. In contrast, DNA lysates were prepared with a ~3.5-hour Proteinase K protocol (2) or a rapid (~2.5 hour) alkaline lysis method (3) (top panel). In both cases, amplification was performed with wild-type Taq (1.5 hour cycling protocol). Results obtained with the KAPA Mouse Genotyping Kit were equal or better (more specific) than those obtained with other methods, which (depending on the exact DNA extraction protocol used), may take at least four times as long, or up to 1 day to complete.
View data showing comparable or better PCR performance, reduced cost per PCR reaction, and better sensitivity, yield and specificity in multiplex PCR using KAPA Mouse Genotyping Kits compared to other commercially available kits.
Figure 3. Compared to the Competitor Q Kit, KAPA Mouse Genotyping Kits exhibit better sensitivity, yield, and specificity in multiplex PCR. Reactions were performed with annealing at either 50°C or 60°C to demonstrate the effect of using sub-optimal annealing temperatures in genotyping assays.
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*Data on file.
Kits can be stored for up to 18 months at -20°C.
KAPA Mouse Gentoyping Kits include KAPA Express Extract Enzyme (1 U/µL), KAPA Express Extract Buffer (10X), and KAPA2G Fast (or Fast HotStart) Genotyping Mix (2X), which contains DNA Polymerase, reaction buffer with loading dye, dNTPs, and MgCl2 (at a final concentration of 1.5 mM). ReadyMixes for PCR are also sold separately.
| Components |
|---|
| 2X KAPA2G Fast Standard or HotStart Genotyping Mix with dye |
| KAPA Express Extract (1 U/µL) |
| 10X KAPA Express Extract Buffer |
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KAPA Mouse Gentoyping Kits include KAPA Express Extract Enzyme (1 U/µL), KAPA Express Extract Buffer (10X), and KAPA2G Fast (or Fast HotStart) Genotyping Mix (2X), which contains DNA Polymerase, reaction buffer with loading dye, dNTPs, and MgCl2 (at a final concentration of 1.5 mM). ReadyMixes for PCR are also sold separately.
| KAPA Code |
Roche Cat. No |
Description |
Kit Size |
How to buy |
|---|---|---|---|---|
| KK5121 |
07960921001 |
Non-HotStart PCR, without extraction | 500 x 25 µL reactions |
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| KK5621 |
07961316001 |
HotStart PCR, without extraction | 500 x 25 µL reactions |
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| KK7302 |
07961766001 |
Non-HotStart PCR | 500 reactions |
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| KK7352 |
07961804001 |
Standard kit | 500 reactions |
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