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KAPA SYBR FAST qPCR Kits contain the first DNA polymerase engineered via our directed evolution technology to be more tolerant of SYBR Green I dye inhibition. The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis compared to traditional kits.*
Benefits of KAPA SYBR FAST qPCR Kits
Optimal performance in stringent real-time PCR reaction conditions
Improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity compared to other commercial kits*
Figure 1. No bias in amplification efficiency across a wide range of GC contents (44.2 – 62.5%) was observed with KAPA SYBR FAST. Amplification efficiencies achieved for ten housekeeping genes with the KAPA SYBR FAST Universal qPCR Kit were plotted against GC content. The reaction efficiency achieved for each of the ten genes fell within the optimal range of 95% – 105%, independent of the nature of the amplicon.
Figure 2. No bias in amplification efficiency across a wide range of amplicon lengths (86 – 249 bp) was observed with KAPA SYBR FAST. Amplification efficiencies achieved for ten housekeeping genes with the KAPA SYBR FAST Universal qPCR Kit were plotted against amplicon length. The reaction efficiency achieved for each of the ten genes fell within the optimal range of 95 – 105%, independent of the length of the amplicon.
Detect low copy and difficult targets consistently
Improved processivity results in earlier Cq scores
Higher fluorescence detection across varying AT- and GC-rich targets
View data showing more accurate expression quantitation of difficult targets such as DMD (with 23.1% GC content) and NOTCH (with 72.2% GC content) by KAPA SYBR FAST qPCR Kit compared to other commercial kits.
Figure 3. Two target genes were amplified using a 10-fold dilution series of human genomic DNA (16 ng to 1.6 pg per 20 µL reaction) comparing four commercial kits. KAPA SYBR FAST consistently provides high performance for accurate expression quantitation across a range of difficult targets.
Complete real-time PCR runs in just 40 minutes
55% shorter run times with fast cycling protocol
High performance when switching from standard to fast protocols
Kits include KAPA SYBR FAST qPCR Master Mix (2X), which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl2 at a final concentration of 2.5 mM. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes ROX High and ROX Low (both 50X) separately..
Kits include KAPA SYBR FAST qPCR Master Mix (2X), which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl2 at a final concentration of 2.5 mM. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes ROX High and ROX Low (both 50X) separately.
