For purchasing or general questions
KAPA SYBR FAST qPCR Kits contain the first DNA polymerase engineered via our directed evolution technology to be more tolerant of SYBR Green I dye inhibition. The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis compared to traditional kits.*
Refer to Instrument Compatibility Chart for guidance on compatible platforms.
View performance data showing reaction efficiency of 95 – 105% against amplicon length and GC-content using KAPA SYBR FAST qPCR Kits.
Figure 1. No bias in amplification efficiency across a wide range of GC contents (44.2 – 62.5%) was observed with KAPA SYBR FAST. Amplification efficiencies achieved for ten housekeeping genes with the KAPA SYBR FAST Universal qPCR Kit were plotted against GC content. The reaction efficiency achieved for each of the ten genes fell within the optimal range of 95% – 105%, independent of the nature of the amplicon.
Figure 2. No bias in amplification efficiency across a wide range of amplicon lengths (86 – 249 bp) was observed with KAPA SYBR FAST. Amplification efficiencies achieved for ten housekeeping genes with the KAPA SYBR FAST Universal qPCR Kit were plotted against amplicon length. The reaction efficiency achieved for each of the ten genes fell within the optimal range of 95 – 105%, independent of the length of the amplicon.
View data showing more accurate expression quantitation of difficult targets such as DMD (with 23.1% GC content) and NOTCH (with 72.2% GC content) by KAPA SYBR FAST qPCR Kit compared to other commercial kits.
Figure 3. Two target genes were amplified using a 10-fold dilution series of human genomic DNA (16 ng to 1.6 pg per 20 µL reaction) comparing four commercial kits. KAPA SYBR FAST consistently provides high performance for accurate expression quantitation across a range of difficult targets.
For purchasing or general questions
For online ordering
*Data on file.
Kits can be stored for up to 12 months at -20˚C.
Kits include KAPA SYBR FAST qPCR Master Mix (2X), which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl2 at a final concentration of 2.5 mM. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes ROX High and ROX Low (both 50X) separately..
Please reference the Instrument Compatibility Chart for guidance on compatible platforms.
| Kit code | 2X KAPA SYBR FAST qPCR Master Mix (Universal) | 50X KAPA SYBR FAST ROX High | 50X KAPA SYBR FAST ROX Low | 2X KAPA SYBR FAST qPCR Master Mix (with equipment-specific dye) | 2X KAPA SYBR FAST ROX Low Master Mix | |
|---|---|---|---|---|---|---|
| Universal | KK4600 | X | X | X | ||
| KK4601 | ||||||
| KK4602 | ||||||
| ABI Prism | KK4603 | X | ||||
| KK4604 | ||||||
| KK4605 | ||||||
| Bio-Rad iCycler | KK4606 | X | ||||
| KK4607 | ||||||
| KK4608 | ||||||
| Optimized for Roche LightCycler® 480 | KK4609 | X | ||||
| KK4610 | ||||||
| KK4611 | ||||||
| ROX Low | KK4619 | X | ||||
| KK4620 | ||||||
| KK4621 | ||||||
| KK4622 |
For purchasing or general questions
For online ordering
Kits include KAPA SYBR FAST qPCR Master Mix (2X), which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl2 at a final concentration of 2.5 mM. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes ROX High and ROX Low (both 50X) separately.
| KAPA Code |
Roche Cat. No |
Description |
Kit Size |
How to buy |
|---|---|---|---|---|
| KK4600 |
07959362001 |
100 x 20 µL reactions (Universal) | 1 mL |
Login for pricing |
| KK4601 |
07959389001 |
500 x 20 µL reactions (Universal) | 5 mL |
Login for pricing |
| KK4602 |
07959397001 |
1000 x 20 µL reactions (Universal) | 10 mL |
Login for pricing |
| KK4603 |
07959419001 |
100 x 20 µL reactions (ABI Prism®) | 1 mL |
Login for pricing |
| KK4604 |
07959427001 |
500 x 20 µL reactions (ABI Prism) | 5 mL |
Login for pricing |
| KK4605 |
07959435001 |
1000 x 20 µL reactions (ABI Prism) | 10 mL |
Login for pricing |
| KK4606 |
07959443001 |
100 x 20 µL reactions, with fluorescein (Bio-Rad® iCycler™) | 1 mL |
Login for pricing |
| KK4607 |
07959451001 |
500 x 20 µL reactions, with fluorescein (Bio-Rad® iCycler™) | 5 mL |
Login for pricing |
| KK4608 |
07959460001 |
1000 x 20 µL reactions, with fluorescein (Bio-Rad® iCycler™) | 10 mL |
Login for pricing |
| KK4609 |
07959478001 |
100 x 20 µL reactions (optimized for use with Roche LightCycler® 480) | 1 mL |
Login for pricing |
| KK4610 |
07959486001 |
500 x 20 µL reactions (optimized for use with Roche LightCycler® 480) | 5 mL |
Login for pricing |
| KK4611 |
07959494001 |
1000 x 20 µL reactions (optimized for use with Roche LightCycler® 480) | 10 mL |
Login for pricing |
| KK4617 |
07959559001 |
5000 x 20 µL reactions (ABI Prism) | 50 mL |
Login for pricing |
| KK4618 |
07959567001 |
5000 x 20 µL reactions (Universal) | 50 mL |
Login for pricing |
| KK4619 |
07959575001 |
100 x 20 µL reactions (ROX Low) | 1 mL |
Login for pricing |
| KK4620 |
07959583001 |
500 x 20 µL reactions (ROX Low) | 5 mL |
Login for pricing |
| KK4621 |
07959591001 |
1000 x 20 µL reactions (ROX Low) | 10 mL |
Login for pricing |
| KK4622 |
07959605001 |
5000 x 20 µL reactions (ROX Low) | 50 mL |
Login for pricing |
For purchasing or general questions
For online ordering