KAPA SYBR FAST qPCR Kits

For Research Use Only. Not for use in diagnostic procedures.

Overview

KAPA SYBR FAST qPCR Kits contain the first DNA polymerase engineered via our directed evolution technology to be more tolerant of SYBR Green I dye inhibition. The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis compared to traditional kits.*

Benefits of KAPA SYBR FAST qPCR Kits

Optimal performance in stringent real-time PCR reaction conditions
Improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity compared to other commercial kits*

Refer to Instrument Compatibility Chart for guidance on compatible platforms.

Product highlights

Quantitate changes in gene expression more accurately

High reaction efficiency between 95 – 105%, improving accuracy and reproducibility
Unbiased efficiency across a wide range of GC contents and amplicon lengths

View performance data showing reaction efficiency of 95 – 105% against amplicon length and GC-content using KAPA SYBR FAST qPCR Kits.

Figure 1. No bias in amplification efficiency across a wide range of GC contents (44.2 – 62.5%) was observed with KAPA SYBR FAST. Amplification efficiencies achieved for ten housekeeping genes with the KAPA SYBR FAST Universal qPCR Kit were plotted against GC content. The reaction efficiency achieved for each of the ten genes fell within the optimal range of 95% – 105%, independent of the nature of the amplicon.

Figure 2. No bias in amplification efficiency across a wide range of amplicon lengths (86 – 249 bp) was observed with KAPA SYBR FAST. Amplification efficiencies achieved for ten housekeeping genes with the KAPA SYBR FAST Universal qPCR Kit were plotted against amplicon length. The reaction efficiency achieved for each of the ten genes fell within the optimal range of 95 – 105%, independent of the length of the amplicon.

Detect low copy and difficult targets consistently

Improved processivity results in earlier Cq scores
Higher fluorescence detection across varying AT- and GC-rich targets

View data showing more accurate expression quantitation of difficult targets such as DMD (with 23.1% GC content) and NOTCH (with 72.2% GC content) by KAPA SYBR FAST qPCR Kit compared to other commercial kits.

Figure 3. Two target genes were amplified using a 10-fold dilution series of human genomic DNA (16 ng to 1.6 pg per 20 µL reaction) comparing four commercial kits. KAPA SYBR FAST consistently provides high performance for accurate expression quantitation across a range of difficult targets.

Complete real-time PCR runs in just 40 minutes

55% shorter run times with fast cycling protocol
High performance when switching from standard to fast protocols

For purchasing or general questions

For online ordering

*Data on file.

Kits can be stored for up to 12 months at -20˚C.

Kits include KAPA SYBR FAST qPCR Master Mix (2X), which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl2 at a final concentration of 2.5 mM. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes ROX High and ROX Low (both 50X) separately..

Please reference the Instrument Compatibility Chart for guidance on compatible platforms.

Components

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	Kit code	2X KAPA SYBR FAST qPCR Master Mix (Universal)	50X KAPA SYBR FAST ROX High	50X KAPA SYBR FAST ROX Low	2X KAPA SYBR FAST qPCR Master Mix (with equipment-specific dye)	2X KAPA SYBR FAST ROX Low Master Mix
Universal	KK4600	X	X	X
	KK4601
	KK4602
ABI Prism	KK4603				X
	KK4604
	KK4605
Bio-Rad iCycler	KK4606				X
	KK4607
	KK4608
Optimized for Roche LightCycler^® 480	KK4609				X
	KK4610
	KK4611
ROX Low	KK4619					X
	KK4620
	KK4621
	KK4622

For purchasing or general questions

For online ordering

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KAPA Code	Roche Cat. No	Description	Kit Size	How to buy
KK4600	07959362001	100 x 20 µL reactions (Universal)	1 mL	Login for pricing
KK4601	07959389001	500 x 20 µL reactions (Universal)	5 mL	Login for pricing
KK4602	07959397001	1000 x 20 µL reactions (Universal)	10 mL	Login for pricing
KK4603	07959419001	100 x 20 µL reactions (ABI Prism®)	1 mL	Login for pricing
KK4604	07959427001	500 x 20 µL reactions (ABI Prism)	5 mL	Login for pricing
KK4605	07959435001	1000 x 20 µL reactions (ABI Prism)	10 mL	Login for pricing
KK4606	07959443001	100 x 20 µL reactions, with fluorescein (Bio-Rad^® iCycler^™)	1 mL	Login for pricing
KK4607	07959451001	500 x 20 µL reactions, with fluorescein (Bio-Rad^® iCycler^™)	5 mL	Login for pricing
KK4608	07959460001	1000 x 20 µL reactions, with fluorescein (Bio-Rad^® iCycler^™)	10 mL	Login for pricing
KK4609	07959478001	100 x 20 µL reactions (optimized for use with Roche LightCycler^® 480)	1 mL	Login for pricing
KK4610	07959486001	500 x 20 µL reactions (optimized for use with Roche LightCycler^® 480)	5 mL	Login for pricing
KK4611	07959494001	1000 x 20 µL reactions (optimized for use with Roche LightCycler^® 480)	10 mL	Login for pricing
KK4617	07959559001	5000 x 20 µL reactions (ABI Prism)	50 mL	Login for pricing
KK4618	07959567001	5000 x 20 µL reactions (Universal)	50 mL	Login for pricing
KK4619	07959575001	100 x 20 µL reactions (ROX Low)	1 mL	Login for pricing
KK4620	07959583001	500 x 20 µL reactions (ROX Low)	5 mL	Login for pricing
KK4621	07959591001	1000 x 20 µL reactions (ROX Low)	10 mL	Login for pricing
KK4622	07959605001	5000 x 20 µL reactions (ROX Low)	50 mL	Login for pricing

For purchasing or general questions

For online ordering