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KAPA SYBR FAST qPCR Kits

For Research Use Only. Not for use in diagnostic procedures.

KAPA qPCR

Overview

KAPA SYBR FAST qPCR Kits contain the first DNA polymerase engineered via our directed evolution technology to be more tolerant of SYBR Green I dye inhibition. The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis compared to traditional kits.* 

Benefits of KAPA SYBR FAST qPCR Kits

  • Optimal performance in stringent real-time PCR reaction conditions
  • Improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity compared to other commercial kits*

Refer to Instrument Compatibility Chart for guidance on compatible platforms. 

Product highlights

Quantitate changes in gene expression more accurately

  • High reaction efficiency between 95 – 105%, improving accuracy and reproducibility
  • Unbiased efficiency across a wide range of GC contents and amplicon lengths

View performance data showing reaction efficiency of 95 – 105% against amplicon length and GC-content using KAPA SYBR FAST qPCR Kits.

 

Detect low copy and difficult targets consistently

  • Improved processivity results in earlier Cq scores
  • Higher fluorescence detection across varying AT- and GC-rich targets

View data showing more accurate expression quantitation of difficult targets such as DMD (with 23.1% GC content) and NOTCH (with 72.2% GC content) by KAPA SYBR FAST qPCR Kit compared to other commercial kits.

 

Complete real-time PCR runs in just 40 minutes

  • 55% shorter run times with fast cycling protocol
  • High performance when switching from standard to fast protocols

 

For purchasing or general questions

For online ordering

*Data on file.

Kits can be stored for up to 12 months at -20˚C.

Kits include KAPA SYBR FAST qPCR Master Mix (2X), which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl2 at a final concentration of 2.5 mM. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes ROX High and ROX Low (both 50X) separately..

Please reference the Instrument Compatibility Chart for guidance on compatible platforms.

Components

 

   Kit code  2X KAPA SYBR FAST qPCR Master Mix (Universal) 50X KAPA SYBR FAST ROX High 50X KAPA SYBR FAST ROX Low 2X KAPA SYBR FAST qPCR Master Mix (with equipment-specific dye) 2X KAPA SYBR FAST ROX Low Master Mix
Universal KK4600 X X X    
KK4601          
KK4602          
ABI Prism KK4603       X  
KK4604          
KK4605          
Bio-Rad iCycler KK4606       X  
KK4607          
KK4608          
Optimized for Roche LightCycler® 480 KK4609       X  
KK4610          
KK4611          
ROX Low KK4619         X
KK4620          
KK4621          
KK4622          

For purchasing or general questions

For online ordering

Kits include KAPA SYBR FAST qPCR Master Mix (2X), which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl2 at a final concentration of 2.5 mM. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes ROX High and ROX Low (both 50X) separately. 

KAPA Code
Roche Cat. No
Description
Kit Size
How to buy
KK4600
07959362001
100 x 20 µL reactions (Universal) 1 mL
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KK4601
07959389001
500 x 20 µL reactions (Universal) 5 mL
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KK4602
07959397001
1000 x 20 µL reactions (Universal) 10 mL
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KK4603
07959419001
100 x 20 µL reactions (ABI Prism®) 1 mL
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KK4604
07959427001
500 x 20 µL reactions (ABI Prism) 5 mL
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KK4605
07959435001
1000 x 20 µL reactions (ABI Prism) 10 mL
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KK4606
07959443001
100 x 20 µL reactions, with fluorescein (Bio-Rad® iCycler) 1 mL
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KK4607
07959451001
500 x 20 µL reactions, with fluorescein (Bio-Rad® iCycler) 5 mL
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KK4608
07959460001
1000 x 20 µL reactions, with fluorescein (Bio-Rad® iCycler) 10 mL
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KK4609
07959478001
100 x 20 µL reactions (optimized for use with Roche LightCycler® 480) 1 mL
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KK4610
07959486001
500 x 20 µL reactions (optimized for use with Roche LightCycler® 480) 5 mL
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KK4611
07959494001
1000 x 20 µL reactions (optimized for use with Roche LightCycler® 480) 10 mL
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KK4617
07959559001
5000 x 20 µL reactions (ABI Prism) 50 mL
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KK4618
07959567001
5000 x 20 µL reactions (Universal) 50 mL
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KK4619
07959575001
100 x 20 µL reactions (ROX Low) 1 mL
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KK4620
07959583001
500 x 20 µL reactions (ROX Low) 5 mL
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KK4621
07959591001
1000 x 20 µL reactions (ROX Low) 10 mL
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KK4622
07959605001
5000 x 20 µL reactions (ROX Low) 50 mL
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For purchasing or general questions

For online ordering