For purchasing or general questions
KAPA SYBR FAST qPCR Kits
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Overview
KAPA SYBR FAST qPCR Kits contain the first DNA polymerase engineered via our directed evolution technology to be more tolerant of SYBR Green I dye inhibition. The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis compared to traditional kits.*
Benefits of KAPA SYBR FAST qPCR Kits
- Optimal performance in stringent real-time PCR reaction conditions
- Improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity compared to other commercial kits*
Refer to Instrument Compatibility Chart for guidance on compatible platforms.
Product highlights
Quantitate changes in gene expression more accurately
- High reaction efficiency between 95 – 105%, improving accuracy and reproducibility
- Unbiased efficiency across a wide range of GC contents and amplicon lengths
View performance data showing reaction efficiency of 95 – 105% against amplicon length and GC-content using KAPA SYBR FAST qPCR Kits.
Detect low copy and difficult targets consistently
- Improved processivity results in earlier Cq scores
- Higher fluorescence detection across varying AT- and GC-rich targets
View data showing more accurate expression quantitation of difficult targets such as DMD (with 23.1% GC content) and NOTCH (with 72.2% GC content) by KAPA SYBR FAST qPCR Kit compared to other commercial kits.
Complete real-time PCR runs in just 40 minutes
- 55% shorter run times with fast cycling protocol
- High performance when switching from standard to fast protocols
Kits can be stored for up to 12 months at -20˚C.
Kits include KAPA SYBR FAST qPCR Master Mix (2X), which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl2 at a final concentration of 2.5 mM. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes ROX High and ROX Low (both 50X) separately..
Please reference the Instrument Compatibility Chart for guidance on compatible platforms.
Components
Kit code | 2X KAPA SYBR FAST qPCR Master Mix (Universal) | 50X KAPA SYBR FAST ROX High | 50X KAPA SYBR FAST ROX Low | 2X KAPA SYBR FAST qPCR Master Mix (with equipment-specific dye) | 2X KAPA SYBR FAST ROX Low Master Mix | |
---|---|---|---|---|---|---|
Universal | KK4600 | X | X | X | ||
KK4601 | ||||||
KK4602 | ||||||
ABI Prism | KK4603 | X | ||||
KK4604 | ||||||
KK4605 | ||||||
Bio-Rad iCycler | KK4606 | X | ||||
KK4607 | ||||||
KK4608 | ||||||
Optimized for Roche LightCycler® 480 | KK4609 | X | ||||
KK4610 | ||||||
KK4611 | ||||||
ROX Low | KK4619 | X | ||||
KK4620 | ||||||
KK4621 | ||||||
KK4622 |
For purchasing or general questions
For online ordering
Kits include KAPA SYBR FAST qPCR Master Mix (2X), which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl2 at a final concentration of 2.5 mM. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes ROX High and ROX Low (both 50X) separately.
KAPA Code |
Roche Cat. No |
Description |
Kit Size |
How to buy |
---|---|---|---|---|
KK4600 |
07959362001 |
100 x 20 µL reactions (Universal) | 1 mL |
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KK4601 |
07959389001 |
500 x 20 µL reactions (Universal) | 5 mL |
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KK4602 |
07959397001 |
1000 x 20 µL reactions (Universal) | 10 mL |
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KK4603 |
07959419001 |
100 x 20 µL reactions (ABI Prism®) | 1 mL |
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KK4604 |
07959427001 |
500 x 20 µL reactions (ABI Prism) | 5 mL |
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KK4605 |
07959435001 |
1000 x 20 µL reactions (ABI Prism) | 10 mL |
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KK4606 |
07959443001 |
100 x 20 µL reactions, with fluorescein (Bio-Rad® iCycler™) | 1 mL |
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KK4607 |
07959451001 |
500 x 20 µL reactions, with fluorescein (Bio-Rad® iCycler™) | 5 mL |
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KK4608 |
07959460001 |
1000 x 20 µL reactions, with fluorescein (Bio-Rad® iCycler™) | 10 mL |
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KK4609 |
07959478001 |
100 x 20 µL reactions (optimized for use with Roche LightCycler® 480) | 1 mL |
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KK4610 |
07959486001 |
500 x 20 µL reactions (optimized for use with Roche LightCycler® 480) | 5 mL |
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KK4611 |
07959494001 |
1000 x 20 µL reactions (optimized for use with Roche LightCycler® 480) | 10 mL |
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KK4617 |
07959559001 |
5000 x 20 µL reactions (ABI Prism) | 50 mL |
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KK4618 |
07959567001 |
5000 x 20 µL reactions (Universal) | 50 mL |
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KK4619 |
07959575001 |
100 x 20 µL reactions (ROX Low) | 1 mL |
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KK4620 |
07959583001 |
500 x 20 µL reactions (ROX Low) | 5 mL |
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KK4621 |
07959591001 |
1000 x 20 µL reactions (ROX Low) | 10 mL |
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KK4622 |
07959605001 |
5000 x 20 µL reactions (ROX Low) | 50 mL |
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For purchasing or general questions
For online ordering