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For Research Use Only. Not for use in diagnostic procedures.
KAPA SYBR FAST One-Step qRT-PCR Kits are designed for optimal performance in stringent qRT-PCR reaction conditions, exhibiting improvements in sensitivity, specificity, reaction efficiency, and fluorescence. The kits contain an M-MuLV reverse transcriptase, an RNase inhibitor, and a novel DNA polymerase engineered through our directed evolution technology.
View data showing increased signal and reaction efficiency using KAPA SYBR FAST One-Step qRT-PCR Kit compared to a competitor kit.
View data showing high qPCR performance using KAPA SYBR FAST One-Step qRT-PCR Kit across varying lengths of RNA targets.
View performance data for KAPA SYBR FAST One-Step qRT-PCR Kit showing high and uniform efficiency across varying dilutions of target.
For purchasing or general questions
For online ordering
*Data on file.
Kits can be stored for up to 6 months at -20˚C.
Kits include KAPA RT Mix, dUTP, and KAPA SYBR FAST qPCR Master Mix, which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl2 at a final concentration of 2.5 mM. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes 50X ROX High and 50X ROX Low separately.
Please reference the Instrument Compatibility Chart for guidance on compatible platforms.
Kit code | 50X KAPA SYBR FAST ROX High | 50X KAPA SYBR FAST ROX Low | 2X KAPA SYBR FAST qPCR Master Mix (Universal) | 2X KAPA SYBR FAST qPCR Master Mix (with equipment-specific dye) | 50X KAPA RT Enzyme Mix | 10mM dUTP | |
---|---|---|---|---|---|---|---|
Universal | KK4650 | X | X | X | X | X | |
KK4651 | |||||||
KK4652 | |||||||
Bio-Rad iCycler | KK4670 | X | X | X | |||
KK4671 | |||||||
KK4672 | |||||||
Optimized for Roche LightCycler® 480 | KK4680 | X | X | X | |||
KK4681 | |||||||
KK4682 |
For purchasing or general questions
For online ordering
Kits include KAPA RT Mix, dUTP, and KAPA SYBR FAST qPCR Master Mix, which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl2 at a final concentration of 2.5 mM. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes 50X ROX High and 50X ROX Low separately.
KAPA Code |
Roche Cat. No |
Description |
Kit Size |
How to buy |
---|---|---|---|---|
KK4650 |
07959613001 |
100 x 20 µL reactions (Universal) | 1 mL |
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KK4651 |
07959621001 |
500 x 20 µL reactions (Universal) | 5 mL |
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KK4652 |
07959630001 |
1000 x 20 µL reactions (Universal) | 10 mL |
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KK4670 |
07959702001 |
100 x 20 µL reactions, with fluorescein (Bio-Rad® iCycler™) | 1 mL |
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KK4671 |
07959729001 |
500 x 20 µL reactions, with fluorescein (Bio-Rad iCycler) | 5 mL |
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KK4672 |
07959737001 |
1000 x 20 µL reactions, with fluorescein (Bio-Rad iCycler) | 10 mL |
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KK4681 |
07959761001 |
500 x 20 µL reactions (optimized for use with Roche LightCycler 480) | 5 mL |
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For purchasing or general questions
For online ordering