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For Research Use Only. Not for use in diagnostic procedures.
Overview
KAPA SYBR FAST One-Step qRT-PCR Kits are designed for optimal performance in stringent qRT-PCR reaction conditions, exhibiting improvements in sensitivity, specificity, reaction efficiency, and fluorescence. The kits contain an M-MuLV reverse transcriptase, an RNase inhibitor, and a novel DNA polymerase engineered through our directed evolution technology.
Benefits of KAPA SYBR FAST One-Step qRT-PCR Kits
Rapid one-step, one-tube RNA quantification, reducing experimental variation and contamination
A convenient qRT-PCR protocol
Resources
Product highlights
Increase signal and reaction efficiency
Consistently higher amplification efficiencies *
Higher fluorescence, earlier Cq values and improved reaction efficiencies compared to other options*
View data showing increased signal and reaction efficiency using KAPA SYBR FAST One-Step qRT-PCR Kit compared to a competitor kit.
Figure 1. RRMI gene (94 bp, 45.7% GC) amplified from 10-fold dilution series of RNA (100 ng to 10 pg per 20 µL reaction) isolated from human placenta. KAPA SYBR FAST One-Step qRT-PCR Kit demonstrates earlier Cq values, higher fluorescence, and optimal reaction efficiencies.
Figure 2. RRMI gene (94 bp, 45.7% GC) amplified from 10-fold dilution series of RNA (100 ng to 10 pg per 20 µL reaction) isolated from human placenta. KAPA SYBR FAST One-Step qRT-PCR Kit demonstrates earlier Cq values, higher fluorescence, and optimal reaction efficiencies.
Figure 3. RRMI gene (94 bp, 45.7% GC) amplified from 10-fold dilution series of RNA (100 ng to 10 pg per 20 µL reaction) isolated from human placenta. KAPA SYBR FAST One-Step qRT-PCR Kit demonstrates earlier Cq values, higher fluorescence, and optimal reaction efficiencies.
Figure 4. MS2 bacteriophage RNA targets of increasing length were amplified at 0.8 pg per 20 µL reaction. KAPA SYBR FAST One-Step qRT-PCR Kit maintained high performance across all amplicon lengths.
Figure 5. MS2 bacteriophage RNA targets of increasing length were amplified at 0.8 pg per 20 µL reaction. KAPA SYBR FAST One-Step qRT-PCR Kit maintained high performance across all amplicon lengths.
Figure 6. MS2 bacteriophage RNA targets of increasing length were amplified at 0.8 pg per 20 µL reaction. KAPA SYBR FAST One-Step qRT-PCR Kit maintained high performance across all amplicon lengths.
Accurate interrogation across a wide range of RNA template concentrations
View performance data for KAPA SYBR FAST One-Step qRT-PCR Kit showing high and uniform efficiency across varying dilutions of target.
Figure 7. 279 bp fragment amplified in replicates of 6 from a 10-fold dilution series of commercial MS2 bacteriophage RNA (80 pg to 0.8 fg per 20 µL reaction). Reaction efficiency of 95% calculated for all 6 replicates per data.
1 pg–1 µg total RNA, 10 fg–100 ng poly(A)RNA, 10-1x108 copies viral RNA
Kits can be stored for up to 6 months at -20˚C.
Kits include KAPA RT Mix, dUTP, and KAPA SYBR FAST qPCR Master Mix, which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl2 at a final concentration of 2.5 mM. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes 50X ROX High and 50X ROX Low separately.
Kits include KAPA RT Mix, dUTP, and KAPA SYBR FAST qPCR Master Mix, which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl2 at a final concentration of 2.5 mM. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes 50X ROX High and 50X ROX Low separately.
