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For Research Use Only. Not for use in diagnostic procedures.
KAPA2G Robust PCR Kits contain a second-generation DNA polymerase engineered to solve inconsistent amplification across a broad range of amplicon types (GC- and AT-rich). The high performance of the DNA polymerase is ideally suited for challenging PCR applications and difficult samples, eliminating the need for optimization using multiple enzymes and protocols.
View data showing 100% success* in PCR amplifications of amplicons with high GC content (up to 84%) using KAPA2G Robust PCR Kits and 66% success using wild-type Taq polymerase.
View data showing more efficient amplification of a plasmid DNA sample in the presence of PCR inhibitors such as NaCl, urea, ethanol and SDS using KAPA2G Robust PCR Kits compared to wild type Taq polymerase.
View data showing superior PCR amplification of E. coli and yeast colonies using KAPA2G Robust PCR Kits compared to wild-type Taq polymerase.
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*Data on file.
Kits can be stored for up to 12 months at -20˚C.
Standard kits include KAPA2G Robust (5 U/μL), KAPA2G Buffer A, KAPA2G Buffer B and KAPA2G GC Buffer (all at 5X, with 1.5 mM MgCl2 at 1X), KAPA Enhancer 1 (5X), and supplemental MgCl2 (25 mM). Kit options include HotStart and dNTP Mix (10 mM each).
ReadyMix formulations (2X) of KAPA2G Robust HotStart DNA Polymerase are also available. These contain DNA Polymerase, reaction buffer (with or without dye), dNTPs and MgCl2 (at a final concentration of 2 mM).
Components |
---|
KAPA2G Robust Standard or HotStart DNA Polymerase (5 U/µL) |
5X KAPA2G Buffer A with MgCl2 |
5X KAPA2G Buffer B with MgCl2 |
5X KAPA2G GC Buffer with MgCl2 |
5X KAPA Enhancer 1 |
MgCl2 (25 mM) |
dNTP Mix (10 mM each dNTP; optional) |
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KAPA Code |
Roche Cat. No |
Description |
Kit Size |
How to buy |
---|---|---|---|---|
KK5004 |
07960743001 |
With dNTPs | 100 U |
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KK5005 |
07960751001 |
With dNTPs | 250 U |
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KK5023 |
07960859001 |
Standard kit | 100 U |
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KK5024 |
07960867001 |
Standard kit | 250 U |
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KK5515 |
07961057001 |
HotStart PCR Kit | 250 U |
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KK5516 |
07961065001 |
HotStart PCR Kit, with dNTPs | 250 U |
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KK5517 |
07961073001 |
HotStart PCR Kit | 500 U |
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KK5518 |
07961081001 |
HotStart PCR Kit, with dNTPs | 500 U |
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KK5522 |
07961103001 |
HotStart PCR Kit | 100 U |
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KK5525 |
07961120001 |
HotStart PCR Kit | 2500 U |
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KK5532 |
07961197001 |
HotStart PCR Kit, with dNTPs | 100 U |
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KAPA Code |
Roche Cat. No |
Description |
Kit Size |
How to buy |
---|---|---|---|---|
KK5701 |
07961332001 |
HotStart ReadyMix, 100 x 25 µL reactions | 1.25 mL |
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KK5702 |
07961359001 |
HotStart ReadyMix, 500 x 25 µL reactions | 6.25 mL |
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KK5704 |
07961375001 |
HotStart ReadyMix with dye, 100 x 25 µL reactions | 1.25 mL |
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KK5705 |
07961383001 |
HotStart ReadyMix with dye, 500 x 25 µL reactions | 6.25 mL |
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For purchasing or general questions
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