KAPA Long Range PCR Kits

Overview

The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an engineered archaeal (B-family) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long-range and/or sensitive PCR, and possesses 3–4X higher fidelity than Taq polymerase.

Features and Benefits of KAPA Long Range PCR Kits

Hot-start formulation – combined with a proprietary antibody that inactivates the enzyme until the first denaturation step
Elimination of spurious amplification products and increase in reaction efficiency and sensitivity
Supplied in a 2X ReadyMix format with a loading dye, allowing direct loading of PCR products onto agarose gels with no extra steps for adding loading/tracking dye
Contain all the components required for PCR except primers and template

Product highlights

Amplification of long targets with high sensitivity

High sensitivity and specificity up to 15 kb
High yields with low input DNA

View data showing highly specific amplification of targets with different amplicon lengths using KAPA Long Range PCR kits.

Figure 1. Amplification of template dilution of human genomic DNA starting with 50 ng, 10 ng, 2 ng, 400 pg per 25 µL. Amplicons were 737 bp, 1.3 kb, 3.3 kb, and 4.5 kb in lengths. 35 cycles, 72˚C extension temperature.

Figure 2. Amplification of template dilution of human genomic DNA starting with 50 ng, 10 ng, 2 ng, 400 pg per 25 µL. Amplicons were 7.5 kb, 10.5 kb, 13 kb, and 15 kb in lengths. 35 cycles, 68˚C extension temperature.

Superior PCR performance with complex targets compared to competitor kits

Outperforms competitor kits in terms of high yield, sensitivity and specificity*

View data showing greater sensitivity and specificity using KAPA Long Range PCR kits compared to competitor kits.

Figure 3. Amplification of 4.5 kb fragment from 50 ng, 10 ng, 2 ng, and 400 pg of human genomic DNA per 25 µL reaction. Lanes: (M) Marker, (1-4) KAPA Long Range HotStart, (5-8) Competitor T, (13-16) Competitor R.

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*Data on file.

Specifications

Starting Material

Genomic DNA, cDNA, plasmid, lambda
Input Amount

< 100 ng for genomic DNA, cDNA < 10 ng for less complex DNA

Kits may be stored for up to 18 months at -20˚C.

Kits include KAPA Long Range or KAPA Long Range HotStart DNA Polymerase (5 or 2.5 U/μL), MgCl2-free KAPA Long Range Buffer (5X), high-quality dNTP Mix (10 mM each) and MgCl2 (25 mM). KAPA Long Range HotStart is also available in KAPA Long Range HotStart ReadyMix with dye (2X), which contains DNA Polymerase, reaction buffer with loading dye, dNTPs and MgCl2 (at a final concentration of 2 mM).

Specifications

Starting Material

Genomic DNA, cDNA, plasmid, lambda
Input Amount

< 100 ng for genomic DNA, cDNA < 10 ng for less complex DNA

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Components
KAPA Long Range DNA Polymerase (5 U/µL) or KAPA Long Range HotStart DNA Polymerase (2.5 U/µL)
5X KAPA Long Range Buffer (without Mg²⁺)
MgCl₂(25 mM)
dNTP Mix (10 mM each dNTP)

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KAPA Code	Roche Cat. No	Description	Kit Size	How to buy
KK3502	07959150001	HotStart PCR Kit ,with dNTPs	250 U	Login for pricing
KK3503	07959168001	HotStart PCR Kit, with dNTPs	500 U	Login for pricing

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KAPA Code	Roche Cat. No	Description	Kit Size	How to buy
KK3601	07959206001	HotStart ReadyMix with dye, 100 x 25 µL reactions	1.25 mL	Login for pricing
KK3602	07959214001	HotStart ReadyMix with dye, 500 x 25 µL reactions	6.25 mL	Login for pricing

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KAPA Long Range PCR Kits

Overview

Features and Benefits of KAPA Long Range PCR Kits

Resources

Product highlights

Specifications

Specifications

Contents