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The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an engineered archaeal (B-family) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long-range and/or sensitive PCR, and possesses 3–4X higher fidelity than Taq polymerase.
View data showing highly specific amplification of targets with different amplicon lengths using KAPA Long Range PCR kits.
Figure 1. Amplification of template dilution of human genomic DNA starting with 50 ng, 10 ng, 2 ng, 400 pg per 25 µL. Amplicons were 737 bp, 1.3 kb, 3.3 kb, and 4.5 kb in lengths. 35 cycles, 72˚C extension temperature.
Figure 2. Amplification of template dilution of human genomic DNA starting with 50 ng, 10 ng, 2 ng, 400 pg per 25 µL. Amplicons were 7.5 kb, 10.5 kb, 13 kb, and 15 kb in lengths. 35 cycles, 68˚C extension temperature.
View data showing greater sensitivity and specificity using KAPA Long Range PCR kits compared to competitor kits.
Figure 3. Amplification of 4.5 kb fragment from 50 ng, 10 ng, 2 ng, and 400 pg of human genomic DNA per 25 µL reaction. Lanes: (M) Marker, (1-4) KAPA Long Range HotStart, (5-8) Competitor T, (13-16) Competitor R.
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*Data on file.
Kits may be stored for up to 18 months at -20˚C.
Kits include KAPA Long Range or KAPA Long Range HotStart DNA Polymerase (5 or 2.5 U/μL), MgCl2-free KAPA Long Range Buffer (5X), high-quality dNTP Mix (10 mM each) and MgCl2 (25 mM). KAPA Long Range HotStart is also available in KAPA Long Range HotStart ReadyMix with dye (2X), which contains DNA Polymerase, reaction buffer with loading dye, dNTPs and MgCl2 (at a final concentration of 2 mM).
| Components |
|---|
| KAPA Long Range DNA Polymerase (5 U/µL) or KAPA Long Range HotStart DNA Polymerase (2.5 U/µL) |
| 5X KAPA Long Range Buffer (without Mg2+) |
| MgCl2 (25 mM) |
| dNTP Mix (10 mM each dNTP) |
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| KAPA Code |
Roche Cat. No |
Description |
Kit Size |
How to buy |
|---|---|---|---|---|
| KK3502 |
07959150001 |
HotStart PCR Kit ,with dNTPs | 250 U |
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| KK3503 |
07959168001 |
HotStart PCR Kit, with dNTPs | 500 U |
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| KAPA Code |
Roche Cat. No |
Description |
Kit Size |
How to buy |
|---|---|---|---|---|
| KK3601 |
07959206001 |
HotStart ReadyMix with dye, 100 x 25 µL reactions | 1.25 mL |
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| KK3602 |
07959214001 |
HotStart ReadyMix with dye, 500 x 25 µL reactions | 6.25 mL |
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