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For Research Use Only. Not for use in diagnostic procedures.
Overview
KAPA PROBE FORCE qPCR Kit contains a highly inhibitor-resistant qPCR master mix that removes the need for DNA purification, enabling streamlined sample-to-Cq workflows. A third generation DNA polymerase engineered through our Directed Evolution technology imparts resistance to blood, tissue, and plant PCR inhibitors. This allows crude samples to be analyzed with comparable accuracy, reproducibility, and sensitivity as purified DNA.
Benefits of KAPA PROBE FORCE qPCR Kit
Direct qPCR from crude blood, tissue, and plant extracts
Sample-to-Cq workflows in <1 hour
High efficiency for accurate, reproducible, and sensitive result
Superior tolerance to carry-over inhibitors
Multiplex compatibility with crude extracts
Resources
Figure 1. Streamlined sample-to-Cq workflows in <1 hour using the KAPA PROBE FORCE kit.
Product highlights
Generate accurate and reproducible results
High reaction efficiency in the presence of PCR inhibitors for reliable data generation
View data showing high reaction efficiency with a variety of difficult samples using KAPA PROBE FORCE qPCR Kits.
Figure 2. High efficiency target amplification. Reaction efficiencies achieved for inhibitor spiked samples were examined and compared to that of purified DNA. Across various inhibitor types, efficiencies remained within 90-110%.
Break through high levels of qPCR inhibitors
Achieve greater levels of sensitivity for inhibited blood, tissue, and plant samples compared to other available kits*
Convert purified DNA assays to crude workflows without observable Cq delays
View data showing broad range of inhibitor resistance and consistent amplification without Cq delays using KAPA PROBE FORCE qPCR Kits.
Figure 3. Broad range of high inhibitor resistance. KAPA PROBE FORCE exhibits consistent and robust amplification across all inhibitors tested, without observable Cq delays. Baseline performance of KAPA PROBE FORCE and competing master mixes was measured by creating standard curves with purified DNA according to each manufacturer’s recommended cycling conditions. Serial dilutions were run in the following ranges: Human: 100ng-10pg, Mouse: 100ng-10pg, Plant: 25ng-8pg, Bacteria: 2ng-2fg. Inhibitors were individually spiked into purified DNA samples at high concentrations to determine their effect on Cq values. Green, <1 deltaCq, yellow, 1-2 deltaCq, orange, 2-3 deltaCq, red, >3 delta Cq. NA, No amplification.
Figure 4. Broad range of high inhibitor resistance. KAPA PROBE FORCE exhibits consistent and robust amplification across all inhibitors tested, without observable Cq delays. Baseline performance of KAPA PROBE FORCE and competing master mixes was measured by creating standard curves with purified DNA according to each manufacturer’s recommended cycling conditions. Serial dilutions were run in the following ranges: Human: 100ng-10pg, Mouse: 100ng-10pg, Plant: 25ng-8pg, Bacteria: 2ng-2fg. Inhibitors were individually spiked into purified DNA samples at high concentrations to determine their effect on Cq values. Green, <1 deltaCq, yellow, 1-2 deltaCq, orange, 2-3 deltaCq, red, >3 delta Cq. NA, No amplification.
Figure 5. Broad range of high inhibitor resistance. KAPA PROBE FORCE exhibits consistent and robust amplification across all inhibitors tested, without observable Cq delays. Baseline performance of KAPA PROBE FORCE and competing master mixes was measured by creating standard curves with purified DNA according to each manufacturer’s recommended cycling conditions. Serial dilutions were run in the following ranges: Human: 100ng-10pg, Mouse: 100ng-10pg, Plant: 25ng-8pg, Bacteria: 2ng-2fg. Inhibitors were individually spiked into purified DNA samples at high concentrations to determine their effect on Cq values. Green, <1 deltaCq, yellow, 1-2 deltaCq, orange, 2-3 deltaCq, red, >3 delta Cq. NA, No amplification.
Figure 6. Crude sample duplex SNP detection. KAPA PROBE FORCE provides accurate genotyping and tight clustering in the presence of crude extracts (A) cotton seed, 0.5M NaOH extraction, (B) grapevine leaf, 75 mM Tris-HCL & 5 mM TCEP extraction, (C) mouse blood, FTA 0.5 mm disc, and (D) mouse tail extracts, NaOH extraction, for rapid SNP analysis.
Figure 7. Crude sample duplex SNP detection. KAPA PROBE FORCE provides accurate genotyping and tight clustering in the presence of crude extracts (A) cotton seed, 0.5M NaOH extraction, (B) grapevine leaf, 75 mM Tris-HCL & 5 mM TCEP extraction, (C) mouse blood, FTA 0.5 mm disc, and (D) mouse tail extracts, NaOH extraction, for rapid SNP analysis.
Figure 8. Crude sample duplex SNP detection. KAPA PROBE FORCE provides accurate genotyping and tight clustering in the presence of crude extracts (A) cotton seed, 0.5M NaOH extraction, (B) grapevine leaf, 75 mM Tris-HCL & 5 mM TCEP extraction, (C) mouse blood, FTA 0.5 mm disc, and (D) mouse tail extracts, NaOH extraction, for rapid SNP analysis.
Figure 9. Crude sample duplex SNP detection. KAPA PROBE FORCE provides accurate genotyping and tight clustering in the presence of crude extracts (A) cotton seed, 0.5M NaOH extraction, (B) grapevine leaf, 75 mM Tris-HCL & 5 mM TCEP extraction, (C) mouse blood, FTA 0.5 mm disc, and (D) mouse tail extracts, NaOH extraction, for rapid SNP analysis.
All real-time qPCR systems regardless of ROX passive reference dye requirement
Starting Material
Crude blood, tissue, and plant DNA extractions, purified gDNA, cDNA
Input Amount
Up to 200 ng
Available Kit Sizes
1 mL, 5 mL, 10 mL, 50 mL
Kits can be stored for up to 12 months at -20ºC. Master mix includes the KAPA3G HotStart DNA polymerase, dNTPs (including dUTP), MgCl2, ROX reference dye, and stabilizers.
