PCR

Overview

Amplification of DNA fragments by DNA polymerase to produce millions of copies

PCR (polymerase chain reaction) utilizes the ability of naturally occurring DNA polymerases to extend short single-stranded primers that hybridize to the target template DNA. Despite its wide utility, most commercially available PCR reagents are remarkably similar, with any performance difference among them being attributed to differences in buffer formulation or enzyme concentration. These reagents contain wild-type enzymes, isolated from nature without modification. Roche offers the KAPA PCR reagents containing DNA polymerase and other enzymes selected through our directed evolution technology to deliver high-quality results for specific PCR applications.

Roche offers the KAPA PCR reagents optimally suited for a variety of applications. KAPA HiFi PCR Kits , which use KAPA HiFi DNA Polymerase engineered for high fidelity and robustness, are used specifically for library amplification.

Available Products by Application
Application	Product	Features and Benefits
Cloning	KAPA Long Range PCR Kits	Amplification of inserts up to 15 kb with high sensitivity and an error rate 4X lower than Taq DNA polymerase. Compatible with TA cloning
Colony PCR	KAPA2G Robust PCR Kits	Improved tolerance to PCR inhibitors results in higher success rates and streamlined workflows with common E. coli and S. cerevisiae strains*
Crude Sample PCR	KAPA2G Robust PCR Kits KAPA3G Plant PCR Kits KAPA Mouse Genotyping Kits	Improved resistance to many inhibitors commonly found in crude samples—such as denatured protein, salts and metabolites—results in higher PCR success rates and more streamlined workflows* Improved resistance to inhibitors co-purified in plant DNA extracts, and amplification directly from plant tissues enable higher-throughput workflows and eliminate the need for expensive and time-consuming purification* Increased throughput and decreased turnaround time* Better performance compared to other available crude extraction methods *
Direct Plant PCR	KAPA3G Plant PCR Kits	Improved resistance to inhibitors co-purified in plant DNA extracts, and amplification directly from plant tissues enable higher-throughput workflows and eliminate the need for expensive and time-consuming purification*
Fast PCR	KAPA2G Fast PCR Kits	Higher specific activity and processivity significantly reduces PCR cycling times by 50% without a loss in performance*
GC-rich PCR	KAPA2G Robust PCR Kits	Robust performance, shorter cycling times, streamlined workflows and higher success rates across a wide variety of template types and amplicons*
Genotyping	KAPA2G Robust PCR Kits KAPA2G Fast PCR Kits KAPA Taq PCR Kits KAPA Mouse Genotyping Kits	Robust performance, shorter cycling times, streamlined workflows and higher success rates across a wide variety of template types and amplicons* Reduced PCR cycling times and improved success rates streamline workflows for routine genotyping* High quality and optimal formulation for routine genotyping applications Increased throughput and decreased turnaround time* Better performance compared to other available crude extraction methods*
High-fidelity PCR	KAPA Long Range PCR Kits	Recommended for the amplification of longer targets up 15 kb with improved sensitivity and an error rate 4X lower than Taq DNA polymerase
Long-range PCR	KAPA Long Range PCR Kits	Recommended for the amplification of longer targets up 15 kb with improved sensitivity and an error rate 4X lower than Taq DNA polymerase*
Multiplex PCR	KAPA2G Fast Multiplex Kits	Increased specific activity and processivity, formulated for highly sensitive and uniform multiplex amplification*
Single-protocol PCR	KAPA2G Robust PCR Kits KAPA2G Fast PCR Kits	Robust performance across a wide range of GC- and AT-rich templates streamlines workflows while reducing turnaround times. Recommended for end-point applications using gel electrophoresis Broad coverage of both AT- and GC-rich targets consolidates protocols and reduces cycling times. Recommended for higher sensitivity detection such as capillary electrophoresis and Sanger sequencing workflows

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KAPA Mouse Genotyping Kits

With a novel thermostable protease and buffer system, the KAPA Mouse Genotyping Kits allow for the extraction of PCR-ready DNA from mouse tissue in as little as 15 minutes.* Kits provide better performance compared to other available crude extraction methods.*

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KAPA2G Robust PCR Kits

Containing a second-generation DNA polymerase engineered to solve inconsistent amplification across a broad range of amplicon types, the KAPA2G Robust PCR Kits offer a robust performance across a wide range of GC-and AT-rich templates

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KAPA2G Fast PCR Kits

A second-generation enzyme engineered for higher processivity and speed, the KAPA2G Fast PCR Kits offer higher specific activity and processivity significantly reduces PCR cycling times by 50% without a loss in performance.*

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KAPA2G Fast Multiplex PCR Kits

Containing a second-generation enzyme derived through our directed evolution technology, the KAPA2G Fast Multiplex PCR Kits provide increased specific activity and processivity,* and are formulated for highly sensitive and uniform multiplex amplification.

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KAPA Express Extract Kits

Allowing for the extraction of PCR-ready DNA in as little as 15 minutes, the KAPA Express Extract Kit, a novel thermostable protease and buffer system, provides a single-tube reaction, minimizing risk of contamination.

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KAPA Long Range PCR Kits

Containing a two-enzyme system that supports long-range and/or sensitive PCR, the KAPA Long Range PCR Kits possess 3-4X higher fidelity than Taq polymerase. Supplied in a 2X ReadyMix format with a loading dye, the kits allow direct loading of PCR products onto agarose gels.

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KAPA3G Plant PCR Kits

Containing a novel third-generation enzyme engineered to improve toleranceto inhibitors co-purified in plant DNA extracts, the KAPA3G Plant PCR Kits allow fast PCR directly from plant tissues and enable higher throughput workflows and eliminate the need for expensive and time-consuming purification.

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KAPA Taq PCR Kits

With a hot-start formulation combined with a proprietary antibody, the KAPA Taq PCR Kits provide improved sensitivity, specificity and yield compared to other options.*

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KAPA dNTPs

Molecular-grade dNTP mix supplied at a concentration of 10 mM each.

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*Data on file.

Research Use Only. Not for use in diagnostic procedures.
KAPA is a trademark of Roche.