KAPA EvoPlus Kits

For Research Use Only. Not for use in diagnostic procedures.

KAPA EvoPlus Kits


The KAPA EvoPlus Kits offer improved fragmentation performance, insensitivity to fragmentation inhibitors and reduced sequencing artefacts through our streamlined and fully automatable workflow. Bringing a new and upgraded enzymatic fragmentation and library prep solution to the market will enable researchers to achieve higher confidence with increased sequencing efficiency.

The KAPA EvoPlus Kits offer a complete library preparation solution when combined with KAPA Adapters and KAPA HyperPure Beads (sold separately). The kits are compatible with the Illumina sequencing platform and have been qualified with automation methods.


Features and Benefits of KAPA EvoPlus Kits

  • Drastically reduced sequencing artefacts with insensitivity to inhibitors, fully tunable fragmentation and improved library prep performance
  • Simplified, streamlined and automatable workflow, with combined Fragmentation and A-tailing step in ReadyMix formulations
  • Tubes and plated format increases efficiency and convenience
  • Compatible with a wide range of sample types and inputs, and flexibility with respect to fragment size, adapter design and library amplification

Product highlights

Enable superior performance and sequencing results
  • KAPA EvoPlus Kits provide the benefits of enzymatic fragmentation without drawbacks
  • Improved sequencing metrics, allowing higher confidence in data due to reduction in sequencing artefacts

View comparative data showing negligible sequencing artefacts for both KAPA EvoPlus Kits and KAPA HyperPrep Kits (mechanical fragmentation) compared to other suppliers.


Simplified and streamlined workflow
  • Streamlined library prep with combined Fragmentation and A-tailing step
  • ReadyMix formulations, therefore fewer reagents and hands-on-time
  • Tubes and plated format, increased efficiency and convenience
  • Manual and automation friendly
  • Reduces complexity of workflow and provides greater peace of mind
  • Validated with the KAPA HyperCap Workflow
Tunable and trustable fragmentation
  • Library insert sizes adjustable by varying fragmentation time
  • Reproducible insert sizes across a range of GC content and DNA input amounts
  • No impact to fragmentation - insensitive to EDTA (up to 2 mM), as well as numerous other inhibitors

See performance data showing tunability and consistency in DNA library insert sizes with samples from different input amounts and DNA dilution buffer.

Minimal sequence coverage bias
  • Lower sequence bias when compared to other enzymatic fragmentation methods
  • Less bias leads to more uniform sequencing coverage and reduced sequencing costs


The GC distribution for KAPA EvoPlus libraries are equivalent to KAPA HyperPrep libraries which use mechanically sheared DNA (considered industry gold-standard) as the input.

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*Data on file.
All graphic data is on file, unless otherwise noted.


  • Compatible Platform

    All Illumina sequencing instruments

  • Library Type


  • Starting Material

    genomic DNA

  • Input Amount

    10 ng – 500 ng

  • Kit storage

    -15°C to -25°C

Cap or plate colour Component Name

Included in KAPA EvoPlus Kits

(09420037001, 09420053001, 09420339001, 09420428001)

Included in KAPA EvoPlus Kit (PCR-free)

(09420045001, 09420304001, 09420371001, 09420436001)

Red KAPA FragTail ReadyMix Yes Yes
Yellow KAPA Ligation ReadyMix Yes Yes
Green KAPA HiFi HS ReadyMix (2X) Yes No
KAPA Library Amplification Primer Mix (10X) for Illumina sold separately
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View Full Table


Kit Code Roche Cat. No Description Kit Size
N/A 09420037001 KAPA EvoPlus Kit 24 rxn
N/A 09420053001 KAPA EvoPlus Kit 96 rxn
N/A 09420339001 KAPA EvoPlus Kit 384 rxn
N/A 09420428001 KAPA EvoPlus Kit, 96 well plate 96 rxn
N/A 09420045001 KAPA EvoPlus Kit (PCR-free) 24 rxn
N/A 09420304001 KAPA EvoPlus Kit (PCR-free) 96 rxn
N/A 09420371001 KAPA EvoPlus Kit (PCR-free) 384 rxn
N/A 09420436001 KAPA EvoPlus Kit (PCR-free), 96 well plate 96 rxn

Accessory Products

All KAPA Adapter Kits contain KAPA Adapter Dilution Buffer and three additional sealing films to support multiple use. KAPA Adapter Dilution Buffer (08278539001) is also available separately, if required.

Kit Code Roche Cat. No Description Kit Size
KK8007 08963835001 KAPA HyperPure Beads (5 mL) 5 mL
KK8008 08963843001 KAPA HyperPure Beads (30 mL) 30 mL
KK8009 08963851001 KAPA HyperPure Beads (60 mL) 60 mL
KK8011 08963878001 KAPA HyperPure Beads (4 x 60 mL) 4 x 60 mL
KK8010 08963860001 KAPA HyperPure Beads (450 mL) 450 mL
KK8727 08861919702 KAPA Unique Dual-Indexed Adapters Kit (15μM) 96 adapters x 20 μL each
KK8721 08278539001 KAPA Adapter Dilution Buffer (25 mL) 25mL
N/A 09063781001 KAPA Universal Adapter, 15μM 960 μL 96 samples
N/A 09063790001 KAPA Universal Adapter, 15μM 4x960 μL 384 samples
N/A 9329862001 KAPA Universal UMI Adapter, 960 μL 96 samples
N/A 09329889001 KAPA Universal UMI Adapter, 4x960 μL 384 samples
N/A 09134336001 KAPA UDI Primer Mixes, 1-96, 96 rxn 96 samples
N/A 09329838001 KAPA UDI Primer Mixes, 97-192, 96 rxn 96 samples
N/A 09329846001 KAPA UDI Primer Mixes, 193-288, 96 rxn 96 samples
N/A 09329854001 KAPA UDI Primer Mixes, 289-384, 96 rxn 96 samples
Additional pack sizes are available for KAPA HyperPure Beads. Please speak to your local sales representative.
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  • Whole-genome shotgun sequencing
  • Whole-exome or targeted sequencing, using KAPA HyperCap workflow v3.x

The library construction process, from enzymatic fragmentation to final library, can be performed in ~ 2 hours depending on experience, the number of samples being processed, and whether or not library amplification is performed. If necessary, the protocol may be paused safely after completion of the post-ligation cleanup or after post-amplification cleanup.

Purified, adapter-ligated library DNA may be stored at 2°C to 8°C for 1 - 2 weeks, or at -15°C to -25°C for 1 month before amplification, target capture and/or sequencing.

Conditioning solution not required for the KAPA EvoPlus Workflow. KAPA FragTail Readymix is insensitive to inhibitors.

KAPA Adapters are recommended for use with the KAPA EvoPlus Kits. For assistance with third- party adapter compatibility and ordering, please contact Technical Support for guidelines on the formulation of user-supplied library amplification primers.

Please refer to the KAPA UDI Adapters and KAPA HyperPlex Adapters Technical Data Sheets for information about barcode sequences, pooling, kit configurations, formulation, and dilution for different KAPA DNA and RNA library preparation kits and inputs.

KAPA Adapters undergo extensive qPCR- and sequencing-based functional and QC testing to confirm:

  • optimal library construction efficiency
  • minimal levels of adapter-dimer formation
  • nominal levels of barcode cross-contamination

Library construction efficiency and adapter-dimer formation are assessed in a low-input library construction workflow. The conversion rate achieved in the assay indicates library construction efficiency. This is calculated by measuring the yield of the adapter-ligated library (before any amplification) by qPCR (using the KAPA Library Quantification Kit), and expressing this as a % of input DNA. To assess adapter-dimer formation, a modified library construction protocol designed to measure adapter dimer with high sensitivity is used.

The novel, one-tube KAPA EvoPlus chemistry leads to less adapter-dimer formation and carry-over. A single bead-based cleanup after adapter ligation is sufficient to remove unused adapter and adapter dimer, even at the high adapter-insert molar ratios recommended for low-input applications. If necessary, a second post-ligation (or size selection step) cleanup may be included to remove all traces of unused adapter and adapter-dimer, especially for PCR-free workflows.

Depending on the amount of library material required for your application, it may be possible to omit library amplification. In such cases, it is important to ensure that your adapters are designed to support sample indexing (where required), cluster amplification and sequencing. Omitting library amplification further streamlines the workflow and reduces overall library preparation time.

No, the KAPA Library Amp Primers will be available separately. This is different from our on-market KAPA HyperPrep and HyperPlus Kits. The rationale here is that because the KAPA EvoPlus Kits are optimized for WGS and WES workflows, when combined with our KAPA HyperCap workflow v3.x, the customer will use the truncated KAPA HyperPlex adapters with indexing by PCR (i.e., KAPA Universal Adapter + KAPA UDI Primer Mixes). Also, we know that many customers use their own 3rd party adapters with indexing by PCR.

If cycled to completion (not recommended) a single 50 μL KAPA HiFi library amplification reaction can produce 8 - 10 μg of amplified library. To minimize over-amplification and associated undesired artefacts, the number of amplification cycles should be tailored to produce the optimal amount of amplified library required for downstream processes. This is typically in the range of 250 ng - 1.5 μg of final, amplified library.

Quantification of adapter-ligated libraries prior to library amplification can greatly facilitate the optimization of library amplification parameters, particularly when a library construction workflow is first established or optimized.

Library size distribution, and the absence of primer dimers and/or over-amplification products, should be confirmed by means of an electrophoretic method. KAPA Library Quantification Kits are recommended for qPCR-based quantification of libraries prior to pooling for target capture or sequencing. qPCR-based quantification of adapter-ligated libraries (prior to library amplification) can provide useful data for protocol optimization and troubleshooting.

Upon receipt, immediately store enzymes and reaction buffers at -15℃ to -25℃ in a constant-temperature freezer. When stored under these conditions and handled correctly, the kit components will retain full activity until the expiry date indicated on the kit label.

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