KAPA HyperPlus Kits

For Research Use Only. Not for use in diagnostic procedures.

KAPA Hyperplus product
Features and Benefits of KAPA HyperPlus Kits
  • Fully automatable DNA library construction in 1.5–2.5 hours with low-bias fragmentation
  • Industry-leading conversion rates and library complexity, particularly for FFPE DNA
  • PCR-free workflows from lower inputs and improved sequence coverage in workflows with PCR
  • Compatibility with a wide range of sample types and inputs
Hyperplus Workflow

Product highlights

Industry-leading library construction efficiency with integrated workflow
  • Conversion rates >80% from ≥ 100 ng input
  • Superior performance across a range of DNA input amounts
  • Validated with the HyperCap Workflow v3.0

View data for higher conversion of input DNA by the KAPA HyperPlus Kit compared to Illumina TrueSeq Nano DNA Library Prep Kit or Covaris-sheared DNA with KAPA HyperPrep Kit.

Tunable and low-bias enzymatic fragmentation
  • Library insert sizes adjustable from 150–800 bp by varying fragmentation time or temperature
  • Robust and reproducible fragmentation across a range of GC content and DNA input amounts and sample types

See performance data showing tunability and consistency in DNA library insert sizes with samples from different species, and comparative data showing minimal fragmentation bias by KAPA HyperPlus kits compared to Nextera Rapid Capture Exome Kit as well as KAPA HyperPrep Kit with Covaris shearing.

Superior coverage uniformity and improved coverage of challenging regions
  • Higher coverage uniformity and generation of libraries with similar or better quality compared to libraries prepared with Covaris-fragmented DNA

View comparative data showing better sequence coverage uniformity and better coverage of GC-rich regions using KAPA HyperPlus Kit compared to HyperPrep Kits with Covaris shearing and Nextera Rapid Capture Exome Kit with Illumina.

Improved coverage depth for FFPE samples compared to Covaris-sheared DNA
  • Significant increase in coverage depth compared to Covaris-sheared DNA with HyperPrep Kits
  • Lower duplication rates, more on-target reads and more bases covered compared to HyperPrep Kits using Covaris-sheared DNA

View performance data showing significantly higher coverage for FFPE samples using KAPA HyperPlus kits compared to Covaris-sheared DNA.

Minimal GC bias and higher coverage uniformity facilitating de novo whole genome assembly in microbial whole genome sequencing
  • Lower GC bias when compared to tagmentation and other enzymatic fragmentation methods
  • Higher coverage uniformity
  • Fewer contigs, longer N50 lengths and longer contigs

View coverage uniformity plots and GC-bias plots showing superior performance using KAPA Hyper Plus Kits compared to HyperPrep Kits, NEBNext Ultra DNA Library Prep Kit for Illumina and Nextera XT DNA Library Prep Kit.

GC bias plots

Specifications

  • Compatible Platform

    llumina HiSeq, MiSeq, NextSeq

  • Library Type

    DNA

  • Starting Material

    genomic DNA, FFPE DNA, PCR amplicons

  • Input Amount

    1 ng – 1 µg

Kits can be stored for up to 6 months at -20ºC.

Kits contain KAPA Frag Enzyme, KAPA Frag Buffer (10X), KAPA Frag Conditioning Solution, KAPA End Repair & A-Tailing Buffer, KAPA End Repair & A-Tailing Enzyme, KAPA Ligation Buffer, KAPA DNA Ligase, KAPA HiFi HotStartReadyMix and KAPA Library Amplification Primer Mix.

Components

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Components

  ROCHE CAT. NO KAPA Frag
Enzyme
KAPA Frag
Buffer
KAPA Frag
Conditioning
Solution
End Repair 
& A-Tailing
Buffer
End Repair
& A-Tailing
Enzyme
Ligation
Buffer
DNA
Ligase
KAPA HiFi
HotStart
Ready Mix
KAPA
Library
Amplification
Primer Mix
With Library Amplification 07962380001
X X X X X X X X X
07962401001                  
07962428001                  
Without amplification 07962398001 X X X X X X X X X
07962410001                  
07962436001                  
KAPA Code
Roche Cat. No
Description
Kit Size
How to buy
KK8510
07962380001
With Library Amplification 8 reactions
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KK8511
07962398001
PCR-Free 8 reactions
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KK8512
07962401001
With Library Amplification 24 reactions
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KK8513
07962410001
PCR-Free 24 reactions
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KK8514
07962428001
With Library Amplification 96 reactions
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KK8515
07962436001
PCR-Free 96 reactions
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Accessory Products

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Accessory Products

KAPA Single-Indexed Adapter Set A contains indices 2, 4, 5, 6, 7, 12, 13, 14, 15, 16, 18 and 19, whereas Set B contains indices 1, 3, 8, 9, 10, 11, 20, 21, 22, 23, 25, 27. All KAPA Single- and Dual-Indexed Adapter Kits contain KAPA Adapter Dilution Buffer. KAPA Dual-Indexed Adapter Kits also contain three additional sealing films to support multiple use.

KAPA Single-Indexed Adapter Set A contains indices 2, 4, 5, 6, 7, 12, 13, 14, 15, 16, 18 and 19, whereas Set B contains indices 1, 3, 8, 9, 10, 11, 20, 21, 22, 23, 25, 27. All KAPA Single- and Dual-Indexed Adapter Kits contain KAPA Adapter Dilution Buffer. KAPA Dual-Indexed Adapter Kits also contain three additional sealing films to support multiple use.

Kit Code Roche Cat. No Description Kit Size How to Buy
KK8000
07983271001
KAPA Pure Beads (5 mL) 5 mL
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KK8001
07983280001
KAPA Pure Beads (30 mL) 30 mL
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KK8002
07983298001
KAPA Pure Beads (60 mL) 60 mL
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KK8721
08278539001
KAPA Adapter Dilution Buffer (25 mL) 25 mL
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KK8727
08861919702

KAPA Unique Dual-Indexed Adapter Kit, (15 µM)

96 adapters x 20 µl each Login for pricing