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For Research Use Only. Not for use in diagnostic procedures.
Overview
As a part of the dynamic Roche Integrated NGS workflow portfolio, KAPA HyperPure Beads offer a highly consistent solution for size selection and reaction purification in DNA library construction workflows for next-generation sequencing.
Achieve high recovery of DNA across a wide range of input amounts
Reduce sequencing costs and maximize library diversity with improved bead wash efficiency
Remove undesirable reaction components with fast bead cleanup steps
Choose from five pack sizes for workflow flexibility
Resources
Product highlights
High DNA recovery
Compatible with all KAPA DNA library preparation kits (KAPA HyperPlus, KAPA HyperPrep, KAPA EvoPlus)
Obtain high yields of unfragmented DNA prior to library construction compared to the market leader (Figure 1A)
Recover degraded DNA with high-efficiency (Figure 1B)
Figure 1A. Recovery of unfragmented human gDNA. (n=4)
KAPA HyperPure Beads provide superior recovery performance to Supplier A in DNA workflows.
Highly degraded DNA obtained from FFPE clinical research samples (n=12); moderately degraded DNA obtained from Horizon Reference FFPE DNA (n=9). For both (A) and (B), KAPA HyperPure Beads and Supplier A beads were used at a 3X ratio to clean up DNA inputs of various amounts. Recovery was measured using the Qubit Fluorometer 3 dsDNA HS Assay Kit before and after cleanup. Error bars represent standard deviation.
Figure 1B. Recovery of highly degraded and moderately degraded formalin-fixed paraffin-embedded (FFPE) human genomic DNA.
KAPA HyperPure Beads provide superior recovery performance to Supplier A in DNA workflows.
Highly degraded DNA obtained from FFPE clinical research samples (n=12); moderately degraded DNA obtained from Horizon Reference FFPE DNA (n=9). For both (A) and (B), KAPA HyperPure Beads and Supplier A beads were used at a 3X ratio to clean up DNA inputs of various amounts. Recovery was measured using the Qubit Fluorometer 3 dsDNA HS Assay Kit before and after cleanup. Error bars represent standard deviation.
Figure 2A. Postfragmentation size selection of input DNA. High-quality hgDNA was mechanically fragmented with a Covaris E220 Focused Ultrasonicator using conditions optimized to yield a fragment length of 250 – 400 bp.
Figure 2B. Post-ligation size selection. Libraries were prepared from 100 ng of fragmented input DNA using the KAPA HyperPrep Kit and KAPA Unique Dual-Index Adapters.
Figure 2C. Post-amplification size selection. Libraries were PCRamplified and final amplified libraries were subjected to size selection.
