Figure 7. Industry-leading fidelity confirmed by pyrosequencing. Published error rates of Taq, Taq-based long-range PCR blends, and proofreading DNA polymerases (Q5, New England Biolabs and Phusion, Thermo Scientific) are compared to the error rate of KAPA HiFi HotStart ReadyMix. The error rate of KAPA HiFi HotStart ReadyMix is 50 – 100 times lower than that of Taq. The error rate of KAPA HiFi HotStart ReadyMix was confirmed by deep sequencing on the 454 platform, and is 1 error per 3.54 x 10-6 nucleotides incorporated.