The efficiency of next-generation sequencing (NGS) can be increased greatly by using indexed adapters to barcode samples, making it possible to run many pooled samples on a single sequencing run (multiplexing); the reads are then sorted bioinformatically.
However, some high-throughput sequencers are prone to index mis-assignment (index hopping), which can reduce data quality; unique dual-indexing (UDI) strategies help to mitigate the impact of these events.
Data accuracy—especially for quantitative data—can be further increased by the use of molecular barcoding with unique molecular identifiers (UMIs). UMIs assign each individual input molecule with a short tag that enables sequencing reads in the final data to be traced back to individual input molecules. This enables the identification of PCR duplicates and sequencing artifacts, and the removal of these reads from the final data.
- Deliver high library conversion efficiency for targeted sequencing, PCR-free whole genome sequencing (WGS), with-PCR library preparation workflows, and somatic oncology research with cell-free DNA (cfDNA)
- Mitigate index mis-assignment with KAPA Unique Dual Indexing (UDI) Adapters, which provide dual, non-redundant sample barcode combinations. KAPA UDI Adapters undergo sequencing-based QC testing to reduce the potential for index misassignment resulting from barcode cross-contamination
- Save valuable time and resources with automation-friendly, single-use plate formats for UDI primer mixes