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KAPA EvoPrep Kits

For Research Use Only. Not for use in diagnostic procedures.
KAPA EvoPrep Kits
Simplify high-performance library prep from mechanically fragmented DNA and cfDNA

Achieve high-performing NGS library preparation from mechanically fragmented DNA or cell-free DNA (cfDNA) in a streamlined, automation-friendly workflow with KAPA EvoPrep Kit. The KAPA EvoPrep workflow reduces pipetting and reagent preparation steps and is validated with challenging sample inputs such as cfDNA and FFPET DNA.

KAPA EvoPrep Kit is the next evolution of Roche DNA Library Preparation portfolio, delivering unparalleled convenience for whole-genome sequencing (WGS), whole-exome sequencing (WES) and targeted sequencing applications–including cfDNA applications.

  • Increase efficiency & convenience with automation-friendly ReadyMix reagents and new KAPA EvoT4 ligase, in plated format or in tubes
  • Achieve higher yields & greater library conversion efficiency across a range of sample types and DNA inputs as low as 100 pg
  • Reduce sequencing artifacts & coverage bias
  • Improve performance with challenging sample types such as cfDNA
  • Cover regions of interest with depth & accuracy in target enrichment workflows

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Related products

Technical documents

Increase efficiency & convenience
  • Analyze a greater variety of samples with a wide range of DNA inputs (0.1 ng-500 ng)
  • Reduce hands-on-time & the number of reagent tubes with ReadyMix formulations, available in tubes or automation-friendly plates.
  • Shorten workflow time without compromising results with a 5-minute ligation time
An overview of the streamlined KAPA EvoPrep Workflow with ReadyMixes

Figure 1: KAPA EvoPrep ReadyMix reagents are ready-to-use; no master mix preparation is required. NOTES: KAPA full-length UDI adapters are only available in 96- well plates. KAPA Universal Adapters, which are used in combination with KAPA UDI Primer Mixes and require amplification, are available in plates or tubes. KAPA HyperPure Beads, UDI Adapter Kits & KAPA Library Amplification Primer Mix (10X) are sold separately.

Achieve higher yields & greater library conversion efficiency
  • Reduce duplication rates while increasing sequencing coverage, with fewer amplification cycles needed for downstream processing
  • Achieve successful library construction with clinically relevant samples & PCR-free workflows from as little as 50 ng
Chart showing that KAPA EvoPrep chemistry enables high library conversion across a range of input DNA

Figure 2: KAPA EvoPrep chemistry enables high library conversion across a range of input DNA. 0.1 ng-500 ng of Covaris-sheared human genomic DNA was used to prepare libraries with KAPA Universal Adapters (with KAPA UDI Primer Mixes) at the recommended adapter: insert molar ratio following the KAPA EvoPrep and KAPA HyperPrep Kit Instructions for Use. Data bars represent the mean, and error bars represent standard deviation (n=4). *Non-validated input (outside of the input range) for KAPA HyperPrep Kit—optimized cycle number for KAPA EvoPrep Kit inputs used.

Reduce sequencing artifacts & coverage bias
  • Improve your sequencing metrics with reduced artifacts, allowing higher confidence in your data
  • Minimize coverage bias, increasing uniformity of coverage & potentially decreasing sequencing costs
Chart showing that KAPA EvoPrep produces minimal chimeric reads
Chart showing that KAPA EvoPrep produces minimal strand split artifacts
Chart showing that KAPA EvoPrep Kit yields uniform coverage and low bias in AT-rich and GC-rich regions
Chart showing low duplicate reads (and thus high % of unique molecules) in KAPA EvoPrep libraries

Figure 3: KAPA EvoPrep Kit yields uniform coverage with reduction in sequencing artifacts. PCR-free whole genome libraries were prepared using 100 ng of Covaris-sheared human genomic DNA (NA12878) with the KAPA EvoPrep Kit, Supplier Q & Supplier W, following each supplier’s instructions for use. (A) The KAPA EvoPrep Kit had the lowest percentage of Chimeras present. (B) The percentage of SSARs present in data generated with the KAPA EvoPrep Kit was equivalent to Supplier Q, with Supplier W having the highest percentage of SSARs present. SSARs represent chimeric reads that appear to be derived from non-contiguous portions of the genome.³ (C) The KAPA EvoPrep Kit had uniform coverage and low AT-or GC-bias while Supplier Q had lumpy coverage and coverage hotspots i.e., over-representation of AT-rich region. This could require more sequencing to be performed to achieve the requisite coverage for these regions, which increases cost and turnaround times. (D) KAPA EvoPrep Kit had very low duplication count compared to Supplier Q & Supplier W, thereby having more unique library molecules present1. All libraries were sequenced on an Illumina NovaSeq 6000 (2x150bp) & subsampled to 150 M reads.

Improve performance with challenging sample types such as cfDNA
  • Generate more diverse libraries from challenging sample types such as cfDNA
  • Increase confidence in data due to reduction in sequencing artifacts
Chart showing that with cfDNA, KAPA EvoPrep yields better library diversity compared to other supplier kits
Chart showing that, with cfDNA, KAPA EvoPrep Kit produces fewer chimeric reads compared to other suppliers

Figure 4: KAPA EvoPrep Kit yields a high number of unique library molecules, with fewer chimeric reads. Whole genome sequencing libraries were prepared using 1 ng of cfDNA with the KAPA EvoPrep Kit, Supplier N, Supplier Q, Supplier T & Supplier W, following each supplier’s instructions for use. KAPA EvoPrep Kit generated a higher number of unique library molecules compared to Supplier N, Supplier Q, Supplier T & Supplier W, resulting in higher library diversity & data confidence1. The KAPA EvoPrep Kit generated a low percentage of Chimeras present, resulting in higher data confidence2, with Supplier N, Supplier Q & Supplier W generating a higher percentage of Chimeras present. All libraries were sequenced on an Illumina NovaSeq 6000 (2x150bp) & subsampled to 400 M reads.

Cover regions of interest with depth & accuracy in target enrichment workflows
  • Achieve high specificity with the highest percent of target coverage at ≥1000x
  • Combine high uniformity & high recovery of unique molecules
Starting with 10 ng cfDNA, KAPA EvoPrep Kit yielded the highest percent target coverage at ≥1000X vs other suppliers
Starting with 10 ng cfDNA, KAPA EvoPrep Kit yielded the greatest coverage uniformity compared to other suppliers
Starting with 10 ng cfDNA, KAPA EvoPrep Kit yielded greater genome equivalent recovery vs other suppliers

Figure 5: KAPA EvoPrep Kits yield improved sequencing performance in target capture workflows utilizing cfDNA. 10 ng of cfDNA was used to prepare triplicate libraries with the KAPA EvoPrep Kit, Supplier N, Supplier Q, Supplier T & Supplier W, following each supplier’s instructions for use. Libraries were enriched with the KAPA HyperCap Oncology Panel (214 Kb), following the KAPA HyperCap cfDNA Evolved workflow instructions. The KAPA EvoPrep Kit had the highest percentage of target coverage at ≥1000X compared to other suppliers; the highest percentage of coverage uniformity compared to other suppliers, thereby showcasing the optimal utilization of sequencing throughput by higher uniformity; as well as higher unique molecule recovery compared to Supplier N, Supplier Q, Supplier T & Supplier W, resulting in higher data confidence1. All libraries were sequenced on an Illumina NovaSeq 6000 (2x150bp) and subsampled to 150 M reads.

  1. McNulty et al. Impact of reducing DNA input on next-generation sequencing library complexity and variant detection. The journal of Molecular Diagnostics, Volume 22, Issue 5, May 2020, Pages 720-727.
  2. Chen et al. Characterization and mitigation of artifacts derived from NGS library preparation due to structure-specific sequences in the human genome. BMC Genomics (2024) 25:227.
  3. Haile, et al. (2019). Sources of erroneous sequences and artifact chimeric reads in next generation sequencing of genomic DNA from formalin-fixed paraffin-embedded samples. Nucleic Acids Research, 2019, 7,2. doi: 10.1093/nar/gky1142.

For Research Use Only. Not for use in diagnostic procedures.

HYPERCAP, KAPA HYPERPETE, KAPA, KAPA EVOT4, KAPA EVOPREP & KAPA HYPERPREP are trademarks of Roche.

All other product names and trademarks are the property of their respective owners.

Specifications

  • Compatible Platform

    All Illumina sequencing instruments

  • Library Type

    DNA

  • Starting Material

    Genomic DNA, cfDNA 

  • Input amount

    0.1 ng- 500 ng
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Cap or Plate colour Component Name

Included in KAPA EvoPrep Kits

(10154039001) (10153806001)
(10096039001) (10153814001)

Included in KAPA EvoPrep Kits (VK*)

(10212233702) (10212250702)
(10212268702) (10212276702

Included in KAPA EvoPrep Kits (PCR-free)

(10153849001) (10153857001)
(10153865001) (10154284001)
Blue End Repair and A-Tailing ReadyMix Yes Yes Yes
Yellow Ligation ReadyMix Yes Yes Yes
Green KAPA HiFi HotStart ReadyMix (2X) Yes Yes No
Green KAPA Library Amplification Primer Mix (10X) No Yes No
Kits should be stored at -15 ̊C to -20 ̊C | *Virtual kits
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For Research Use Only. Not for use in diagnostic procedures.

HYPERCAP, KAPA HYPERPETE, KAPA, KAPA EVOT4, KAPA EVOPREP & KAPA HYPERPREP are trademarks of Roche.

All other product names and trademarks are the property of their respective owners.

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Roche Cat. No Description Kit Size (reactions)
10154039001 KAPA EvoPrep Kit, 24 rxn 24
10096039001 KAPA EvoPrep Kit, 96 rxn 96
10153849001 KAPA EvoPrep Kit, 384 rxn 384
10153865001 KAPA EvoPrep Kit, 96 rxn, plate format 96
10153806001* KAPA EvoPrep Kit, 24 rxn (PCR-free) 24
10153814001* KAPA EvoPrep Kit, 96 rxn (PCR-free) 96
10153857001* KAPA EvoPrep Kit, 384 rxn (PCR-free) 384
10154284001* KAPA EvoPrep Kit, 96 rxn, plate format (PCR-free) 96
10212233702** KAPA EvoPrep Kit + KAPA Library Amplification Primer Mix (10X), 24 rxn 24
10212250702** KAPA EvoPrep Kit + KAPA Library Amplification Primer Mix (10X), 96 rxn 96
10212268702** KAPA EvoPrep Kit + KAPA Library Amplification Primer Mix (10X), 384 rxn 384
10212276702** KAPA EvoPrep Kit + KAPA Library Amplification Primer Mix (10X), 96 rxn, plate format 96
There are no Kit Codes for the items above | * KAPA Library Amplification Primer Mix (10X) not included | ** Virtual kits
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Kit Code Roche Cat. No Description Kit Size (reactions)
KK8007 08963835001 KAPA HyperPure Beads (5 mL) 5 mL
KK8008 08963843001 KAPA HyperPure Beads (30 mL) 30 mL
KK8009 08963851001 KAPA HyperPure Beads (60 mL) 60 mL
KK8011 08963878001 KAPA HyperPure Beads (4 x 60 mL) 4 x 60 mL
KK8010 08963860001 KAPA HyperPure Beads (450 mL) 450 mL
KK8727 08861919702 KAPA Unique Dual-Indexed Adapters Kit (15μM) 96 adapters x 20μL each 
KK8721 08278539001 KAPA Adapter Dilution Buffer (25 mL) 25 mL
N/A 09063781001 KAPA Universal Adapter, 15μM 960 μL 96-192* samples
N/A 09063790001 KAPA Universal Adapter, 15μM 960 μL 384-768* samples
N/A 09329862001 KAPA Universal UMI Adapter,960 μL 96-192* samples
N/A 09329889001 KAPA Universal UMI Adapter,4x960 μL 384 samples
N/A 09134336001 KAPA UDI Primer Mixes,1-96, 96 rxn 96 samples
N/A 09329838001 KAPA UDI Primer Mixes, 97-192, 96 rxn 96 samples
N/A 09329846001 KAPA UDI Primer Mixes,193-288, 96 rxn 96 samples
N/A 09329854001 KAPA UDI Primer Mixes, 289-384, 96 rxn 96 samples
*Workflow dependent

Additional pack sizes are available. Please speak to your local sales representative about how we can make it possible for you.

Contact us
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For Research Use Only. Not for use in diagnostic procedures.

HYPERCAP, KAPA HYPERPETE, KAPA, KAPA EVOT4, KAPA EVOPREP & KAPA HYPERPREP are trademarks of Roche.

All other product names and trademarks are the property of their respective owners.

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*KAPA EvoPrep evaluation offer is valid for U.S. based customers only.

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