KAPA RNA HyperPrep Kits

Rapid, robust, and reliable

Overview

RNA library preparation is the critical first step of RNA sequencing (RNA-seq) and KAPA RNA HyperPrep kits offers flexible workflow options with streamlined single-tube single-day workflows that include enrichment of desired transcripts by selective mRNA capture or rRNA depletion. These kits have been optimized for high performance even with degraded and low-input samples, and offer the option of custom RNA depletion. KAPA RNA HyperPrep Kits contain high-quality enzymes, including KAPA HiFi DNA polymerase, developed through our Directed Evolution Technology for constructing RNA libraries with minimal GC bias and even sequence coverage.

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Benefits of KAPA RNA HyperPrep Kits

Depletion of rRNA, user-defined RNA, and globin RNA

Whole-transcriptome sequencing provides a complete view of the transcriptome, including immature and noncoding RNAs. In these workflows, it is important to remove rRNA (up to 95% of the total RNA sample) prior to library prep in order to minimize the number of wasted sequencing reads. This method is effective with degraded RNA inputs not suitable for mRNA capture. Enzymatic depletion can also be used to remove other transcripts, such as globin mRNA and/or other highly abundant RNAs.

mRNA Capture

mRNA capture sequencing is used for the analysis of coding transcripts in eukaryotic samples, allowing researchers to detect and quantify relative expression levels. This workflow enriches for mRNA over non-polyadenylated species such as ribosomal, precursor, and non-coding RNAs.

KAPA RNA Hyperprep provides streamlined, strand-specific library construction.

The novel chemistry employed in KAPA RNA HyperPrep Kits allows for fewer and shorter enzymatic steps, reducing hands-on time and overall library prep time. rRNA depletion with KAPA RiboErase (HMR) or KAPA RiboErase (HMR) Globin Kits adds approximately 2.5 hours to the overall workflow time, whereas mRNA capture adds approximately 1.5 hours. The entire workflow, from input RNA to sequencing-ready library, can easily be completed in a standard workday. All KAPA RNA HyperPrep library construction workflows are automation friendly.

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KAPA RNA HyperPrep Kits Specifications and Comparison

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The first-generation KAPA Stranded RNA-seq Kits are still available for purchase. For more information about these older RNA library kits, please click here.

For Research Use Only. Not for use in diagnostic procedures.
KAPA is a trademark of Roche. Other product names and trademarks are the property of their respective owners.

Product Name	KAPA RNA HyperPrep Kits	KAPA mRNA HyperPrep Kits	KAPA RNA HyperPrep Kits with RiboErase (HMR)	KAPA RNA HyperPrep Kits with RiboErase (HMR) Globin
Library Preparation Duration	4 hours	5.5 hours	6.5 hours	6.5 hours
Sample Input Amount	1-100 ng into library prep	50 ng - 1 µg into mRNA capture	25 ng - 1 µg into rRNA depletion	25 ng - 1 µg into rRNA depletion
Sample Types	High-quality total RNA Degraded or FFPE total RNA Poly(A)-selected and/or depleted RNA	High-quality total RNA	High-quality total RNA Degraded or FFPE total RNA	High-quality total RNA Degraded or FFPE total RNA Whole blood total RNA
Species	Eukaryotic (Animal, Plant, etc.) Prokaryotic (Bacteria, etc.) Viral	Eukaryotic (Animal, Plant, etc.)	Human, Mouse, and Rat Other species can be supported via KAPA RiboDesigner custom rRNA depletion	Human, Mouse, and Rat Other species can be supported via KAPA RiboDesigner custom rRNA depletion
Stranded RNA-sequencing	Yes	Yes	Yes	Yes
Amplification	KAPA HiFi DNA Polymerase Compatible with low bias amplification	KAPA HiFi DNA Polymerase Compatible with low bias amplification	KAPA HiFi DNA Polymerase Compatible with low bias amplification	KAPA HiFi DNA Polymerase Compatible with low bias amplification
Reaction Purification	KAPA Pure Beads included	KAPA Pure Beads included	KAPA Pure Beads included	KAPA Pure Beads included
Multiplexing	Yes. KAPA Adapters are not supplied, but available separately	Yes. KAPA Adapters are not supplied, but available separately	Yes. KAPA Adapters are not supplied, but available separately	Yes. KAPA Adapters are not supplied, but available separately
Applications	Whole-transcriptome sequencing Gene expression with target enrichment Splice junction and gene fusions with target enrichment	Gene expression Annotation Splice junction and gene fusions	Whole-transcriptome Long non-coding RNA Annotation Gene expression Splice junction and gene fusions	Whole-transcriptome Long non-coding RNA Annotation Gene expression Splice junction and gene fusions
Automation Friendly	Yes. Demonstrated and verified on many liquid handling systems. Learn more	Yes. Demonstrated and verified on many liquid handling systems. Learn more	Yes. Demonstrated and verified on many liquid handling systems. Learn more	Yes. Demonstrated and verified on many liquid handling systems. Learn more
Genialis™ Data Analysis and Visualization Solution	Yes Learn more	Yes Learn more	Yes Learn more	Yes Learn more
KAPA RiboDesigner Custom rRNA Depletion Support	N/A	N/A	Yes Learn more	Yes Learn more
Compatible Sequencing Platforms	Illumina Sequencers	Illumina Sequencers	Illumina Sequencers	Illumina Sequencers
Storage Information	Enzymes and buffers can be stored for up to 10 months at -20°C KAPA Pure Beads can be stored for up to 10 months at 4°C	Enzymes and buffers can be stored for up to 10 months at -20°C KAPA Pure Beads can be stored for up to 10 months at 4°C	Enzymes and buffers can be stored for up to 10 months at -20°C KAPA Pure Beads can be stored for up to 10 months at 4°C	Enzymes and buffers can be stored for up to 10 months at -20°C KAPA Pure Beads can be stored for up to 10 months at 4°C