KAPA RNA HyperPrep Kits

Rapid, robust, and reliable

KAPA RNA HyperPrep Kits

Overview

RNA library preparation is the critical first step of RNA sequencing (RNA-seq) and KAPA RNA HyperPrep kits offers flexible workflow options with streamlined single-tube single-day workflows that include enrichment of desired transcripts by selective mRNA capture or rRNA depletion. These kits have been optimized for high performance even with degraded and low-input samples, and offer the option of custom RNA depletion. KAPA RNA HyperPrep Kits contain high-quality enzymes, including KAPA HiFi DNA polymerase, developed through our Directed Evolution Technology for constructing RNA libraries with minimal GC bias and even sequence coverage.

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Benefits of KAPA RNA HyperPrep Kits

Depletion of rRNA, user-defined RNA, and globin RNA

Whole-transcriptome sequencing provides a complete view of the transcriptome, including immature and noncoding RNAs. In these workflows, it is important to remove rRNA (up to 95% of the total RNA sample) prior to library prep in order to minimize the number of wasted sequencing reads. This method is effective with degraded RNA inputs not suitable for mRNA capture. Enzymatic depletion can also be used to remove other transcripts, such as globin mRNA and/or other highly abundant RNAs.

mRNA Capture

mRNA capture sequencing is used for the analysis of coding transcripts in eukaryotic samples, allowing researchers to detect and quantify relative expression levels. This workflow enriches for mRNA over non-polyadenylated species such as ribosomal, precursor, and non-coding RNAs.

KAPA RNA Hyperprep provides streamlined, strand-specific library construction.

The novel chemistry employed in KAPA RNA HyperPrep Kits allows for fewer and shorter enzymatic steps, reducing hands-on time and overall library prep time. rRNA depletion with KAPA RiboErase (HMR) or KAPA RiboErase (HMR) Globin Kits adds approximately 2.5 hours to the overall workflow time, whereas mRNA capture adds approximately 1.5 hours. The entire workflow, from input RNA to sequencing-ready library, can easily be completed in a standard workday. All KAPA RNA HyperPrep library construction workflows are automation friendly.

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KAPA RNA HyperPrep Kits Specifications and Comparison

Product Name KAPA RNA HyperPrep Kits KAPA mRNA HyperPrep Kits KAPA RNA HyperPrep Kits with RiboErase (HMR) KAPA RNA HyperPrep Kits with RiboErase (HMR) Globin
Library Preparation Duration 4 hours 5.5 hours 6.5 hours 6.5 hours
Sample Input Amount 1-100 ng into library prep 50 ng - 1 µg into mRNA capture 25 ng - 1 µg into rRNA depletion 25 ng - 1 µg into rRNA depletion
Sample Types

High-quality total RNA

Degraded or FFPE total RNA

Poly(A)-selected and/or depleted RNA

High-quality total RNA

High-quality total RNA

Degraded or FFPE total RNA

High-quality total RNA

Degraded or FFPE total RNA

Whole blood total RNA

Species

Eukaryotic (Animal, Plant, etc.)

Prokaryotic (Bacteria, etc.)

Viral

Eukaryotic (Animal, Plant, etc.)

Human, Mouse, and Rat

Other species can be supported via KAPA RiboDesigner custom rRNA depletion

Human, Mouse, and Rat

Other species can be supported via KAPA RiboDesigner custom rRNA depletion

Stranded RNA-sequencing Yes Yes Yes Yes
Amplification

KAPA HiFi DNA Polymerase

Compatible with low bias amplification

KAPA HiFi DNA Polymerase

Compatible with low bias amplification

KAPA HiFi DNA Polymerase

Compatible with low bias amplification

KAPA HiFi DNA Polymerase

Compatible with low bias amplification

Reaction Purification KAPA Pure Beads included KAPA Pure Beads included KAPA Pure Beads included KAPA Pure Beads included
Multiplexing Yes.  KAPA Adapters are not supplied, but available separately Yes.  KAPA Adapters are not supplied, but available separately Yes.  KAPA Adapters are not supplied, but available separately Yes.  KAPA Adapters are not supplied, but available separately
Applications

Whole-transcriptome sequencing

Gene expression with target enrichment

Splice junction and gene fusions with target enrichment

Gene expression

Annotation

Splice junction and gene fusions

Whole-transcriptome

Long non-coding RNA Annotation

Gene expression

Splice junction and gene fusions

Whole-transcriptome

Long non-coding RNA Annotation

Gene expression

Splice junction and gene fusions

Automation Friendly Yes.  Demonstrated and verified on
many liquid handling systems.
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Yes.  Demonstrated and verified on
many liquid handling systems.
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Yes.  Demonstrated and verified on many
liquid handling systems.
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Yes.  Demonstrated and verified on many
liquid handling systems.
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Genialis™ Data Analysis and Visualization Solution Yes
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Yes
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Yes
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Yes
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KAPA RiboDesigner Custom rRNA Depletion Support N/A N/A Yes
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Yes
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Compatible Sequencing Platforms Illumina Sequencers Illumina Sequencers Illumina Sequencers Illumina Sequencers
Storage Information

Enzymes and buffers can be stored for up to 10 months at -20°C

 

KAPA Pure Beads can be stored for up to 10 months at 4°C

Enzymes and buffers can be stored for up to 10 months at -20°C

 

KAPA Pure Beads can be stored for up to 10 months at 4°C

Enzymes and buffers can be stored for up to 10 months at -20°C

 

KAPA Pure Beads can be stored for up to 10 months at 4°C

Enzymes and buffers can be stored for up to 10 months at -20°C

 

KAPA Pure Beads can be stored for up to 10 months at 4°C

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The first-generation KAPA Stranded RNA-seq Kits are still available for purchase.  For more information about these older RNA library kits, please click here.