KAPA HiFi Kits

For Research Use Only. Not for use in diagnostic procedures.

Overview

KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high-fidelity polymerases and polymerase blends. KAPA HiFi has been engineered to have an increased affinity for DNA without the need for accessory protein domains. The intrinsic high processivity of KAPA HiFi results in signifcant improvements to yield, sensitivity, speed, target length, and the ability to amplify diffcult templates (e.g., AT- and GC-rich). The error rate of KAPA HiFi PCR Kits is 100X lower than wild-type Taq DNA polymerase.

Product highlights

Achieve the highest fidelity

Increased processivity, strong proofreading activity, and optimized buffer system result in superior accuracy
The error rate of KAPA HiFi is 100X lower than Taq, 40X lower than polymerase blends, 3X lower than Pfu Ultra and 2X lower than Phusion

Improve performance on GC- and AT-rich templates

Achieve higher success rates across targets up to 84% GC content
Higher yields when amplifying AT-rich templates

Amplify longer targets with greater sensitivity

High-fidelity PCR of long and complex genomic templates
Improved sensitivity
High speed allows significantly shorter reaction times for long range PCR

Figure 3. Amplification of hgDNA targets up to 11 kb. Each target was amplified from a descending range of template hgDNA concentrations (50 ng to 0.5 ng per reaction). Reactions (25 µL each) were performed using standard 3-step cycling profiles (35 cycles): 20 sec denaturation, 15 sec annealing, and 30 sec/kb extension time. Total reaction time for the 11 kb amplicon was 3h 50 min. Data on file.

Figure 4. 2 kb lambda phage target amplified from a 10-fold dilution series starting from 10 ng down to 10 fg using KAPA HiFi HotStart DNA Polymerase with Fidelity Buffer, fusion technology polymerase, and polymerase blend systems. All reactions (25 µL each) were performed using manufacturers’ protocols and buffers, with standard 3-step cycling profiles (35 cycles). Data on file.

Figure 5. KAPA HiFi DNA Polymerase exhibits robust performance on difficult templates. A panel of amplicons from human genomic DNA with a GC content ranging from 47% to 84% was used to compare the performance of KAPA HiFi HotStart and competitor kits. A polymerase with fusion technology in GC Buffer and a polymerase blend are only able to amplify targets up to 64% GC content. KAPA HiFi in GC Buffer achieves a 100% success rate with higher yields across all amplicons up to 84% GC content. Data on file.

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*Data on file.

Store kits for 18 months at -20°C.

Kits include KAPA HiFi or KAPA HiFi HotStart DNA Polymerase (1 U/µL), Fidelity Buffer (5X), GC Buffer (5X), high-quality dNTPs (10 mM each) and MgCl₂ (25 mM). Both buffers contain MgCl₂ at a final concentration of 2 mM.

KAPA HiFi HotStart is also available in KAPA HiFi HotStart ReadyMix (2X), which contains DNA Polymerase, reaction buffer, dNTPs and MgCl₂ (at a final concentration of 2.5 mM).

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	KAPA HiFi PCR Kit	KAPA HiFi HotStart PCR Kit	KAPA HiFi HotStart Uracil+ Kit	KAPA HiFI HotStart ReadyMix PCR Kit	KAPA HiFi HotStart Library Amplification Kit	KAPA HiFi HotStart Library Amplification Kit with Primer Mix	KAPA HiFi HotStart Real-Time Library Amplification Kit
HotStart Enzyme	No	Yes	Yes	Yes	Yes	Yes	Yes
Format	MasterMix	MasterMix	ReadyMix	ReadyMix	ReadyMix	ReadyMix with Primer Mix	MasterMix
Kit Contents	KAPA HiFi enzyme KAPA HiFi buffer KAPA Hifi GC buffer Magnesium Chloride dNTPs	KAPA HiFi HotStart enzyme KAPA HiFi buffer KAPA HiFi GC buffer Magnesium Chloride dNTPs	KAPA HiFi HotStart ReadyMix, MgCl₂	KAPA HiFi HotStart ReadyMix	KAPA HiFi HotStart ReadyMix	KAPA HiFi HotStart ReadyMix Amplification Primer Mix	KAPA HiFi HotStart ReadyMix Fluorescent Standards
Recommended Applications	Includes buffers for high-fidelity PCR and difficult targets, including GC-rich templates	Antibody-mediated hot start with buffers for high-fidelity PCR and difficult targets, including GC-rich templates	Antibody-mediated hot start PCR for bisulfite sequencing	Antibody-mediated hot start in a convenient ReadyMix formulation for routine high-fidelity PCR	Antibody-mediated hot start for routine high-fidelity library amplification with user-supplied sequencing primers	Antibody-mediated hot start for routine high-fidelity library amplification with Illumina adapters and indexing primers	Replace post-amplification QC by gel electrophoresis with real-time monitoring of amplification against a target range

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KAPA HiFi PCR Kits

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KAPA Code	Roche Cat. No	Description	Kit Size	How to buy
KK2101	07958838001	With dNTPs	100 U	Login for pricing
KK2102	07958846001	With dNTPs	250 U	Login for pricing
KK2501	07958889001	HotStart PCR Kit, with dNTPs	100 U	Login for pricing
KK2502	07958897001	HotStart PCR Kit, with dNTPs	250 U	Login for pricing
KK2601	07958927001	HotStart ReadyMix, 100 x 25 µL reactions	1.25 mL	Login for pricing
KK2602	07958935001	HotStart ReadyMix, 500 x 25 µL reactions	6.25 mL	Login for pricing
09420398001	09420398001	HiFi HotStart ReadyMix, 9.6ml	9.6 mL	Login for pricing

KAPA HiFi Kits for bisulfite sequencing

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KAPA CODE	ROCHE CAT NO	DESCRIPTION	KIT SIZE
KK2801	7959052001	KAPA HiFi HotStart Uracil+ Kit	50 rxns
KK2802	7959079001	KAPA HiFi HotStart Uracil+ Kit	250 rxns

KAPA HiFi Library Amplification Kits

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KAPA Code	Roche Cat. No	Description	Kit Size	How to buy
KK2611	07958951001	HotStart Ready Mix without primer mix	50 x 50 µL reactions	Login for pricing
KK2612	07958960001	HotStart Ready Mix without primer mix	250 x 50 µL reactions	Login for pricing
KK2620	07958978001	HotStart ReadyMix with primer mix	50 x 50 µL reactions	Login for pricing
KK2621	07958986001	HotStart ReadyMix with primer mix	250 x 50 µL reactions	Login for pricing
KK2702	07959028001	HotStart ReadyMix real-time PCR library amplification, with fluorescent standards	250 x 50 µL reactions	Login for pricing

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KAPA HiFi Kits