KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high-fidelity polymerases and polymerase blends. KAPA HiFi has been engineered to have an increased affinity for DNA without the need for accessory protein domains. The intrinsic high processivity of KAPA HiFi results in signifcant improvements to yield, sensitivity, speed, target length, and the ability to amplify diffcult templates (e.g., AT- and GC-rich). The error rate of KAPA HiFi PCR Kits is 100X lower than wild-type Taq DNA polymerase.
KAPA HiFi Kits
For Research Use Only. Not for use in diagnostic procedures.
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Overview
Product highlights
Achieve the highest fidelity
- Increased processivity, strong proofreading activity, and optimized buffer system result in superior accuracy
- The error rate of KAPA HiFi is 100X lower than Taq, 40X lower than polymerase blends, 3X lower than Pfu Ultra and 2X lower than Phusion
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Improve performance on GC- and AT-rich templates
- Achieve higher success rates across targets up to 84% GC content
- Higher yields when amplifying AT-rich templates
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Amplify longer targets with greater sensitivity
- High-fidelity PCR of long and complex genomic templates
- Improved sensitivity
- High speed allows significantly shorter reaction times for long range PCR
Store kits for 18 months at -20°C.
Kits include KAPA HiFi or KAPA HiFi HotStart DNA Polymerase (1 U/µL), Fidelity Buffer (5X), GC Buffer (5X), high-quality dNTPs (10 mM each) and MgCl2 (25 mM). Both buffers contain MgCl2 at a final concentration of 2 mM.
KAPA HiFi HotStart is also available in KAPA HiFi HotStart ReadyMix (2X), which contains DNA Polymerase, reaction buffer, dNTPs and MgCl2 (at a final concentration of 2.5 mM).
KAPA HiFi PCR Kit | KAPA HiFi HotStart PCR Kit | KAPA HiFi HotStart Uracil+ Kit | KAPA HiFI HotStart ReadyMix PCR Kit | KAPA HiFi HotStart Library Amplification Kit | KAPA HiFi HotStart Library Amplification Kit with Primer Mix | KAPA HiFi HotStart Real-Time Library Amplification Kit | |
---|---|---|---|---|---|---|---|
HotStart Enzyme | No | Yes | Yes | Yes | Yes | Yes | Yes |
Format | MasterMix | MasterMix | ReadyMix | ReadyMix | ReadyMix | ReadyMix with Primer Mix | MasterMix |
Kit Contents | KAPA HiFi enzyme KAPA HiFi buffer KAPA Hifi GC buffer Magnesium Chloride dNTPs |
KAPA HiFi HotStart enzyme KAPA HiFi buffer KAPA HiFi GC buffer Magnesium Chloride dNTPs |
KAPA HiFi HotStart ReadyMix, MgCl2 | KAPA HiFi HotStart ReadyMix | KAPA HiFi HotStart ReadyMix | KAPA HiFi HotStart ReadyMix Amplification Primer Mix |
KAPA HiFi HotStart ReadyMix Fluorescent Standards |
Recommended Applications | Includes buffers for high-fidelity PCR and difficult targets, including GC-rich templates | Antibody-mediated hot start with buffers for high-fidelity PCR and difficult targets, including GC-rich templates | Antibody-mediated hot start PCR for bisulfite sequencing | Antibody-mediated hot start in a convenient ReadyMix formulation for routine high-fidelity PCR | Antibody-mediated hot start for routine high-fidelity library amplification with user-supplied sequencing primers | Antibody-mediated hot start for routine high-fidelity library amplification with Illumina adapters and indexing primers | Replace post-amplification QC by gel electrophoresis with real-time monitoring of amplification against a target range |
For purchasing or general questions
For online ordering
KAPA HiFi PCR Kits
View Full TableKAPA HiFi PCR Kits
KAPA Code |
Roche Cat. No |
Description |
Kit Size |
How to buy |
---|---|---|---|---|
KK2101 |
07958838001 |
With dNTPs | 100 U |
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KK2102 |
07958846001 |
With dNTPs | 250 U |
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KK2501 |
07958889001 |
HotStart PCR Kit, with dNTPs | 100 U |
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KK2502 |
07958897001 |
HotStart PCR Kit, with dNTPs | 250 U |
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KK2601 |
07958927001 |
HotStart ReadyMix, 100 x 25 µL rxn | 1.25 mL |
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KK2602 |
07958935001 |
HotStart ReadyMix, 500 x 25 µL rxn | 6.25 mL |
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09420398001 |
09420398001 |
HiFi HotStart ReadyMix, 9.6ml |
9.6 mL |
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N/A | 09420444001 | HiFi HotStart ReadyMix (96 rxn, Pre-plated format) | 96 rxn | Login for pricing |
KAPA HiFi Kits for bisulfite sequencing
View Full TableKAPA HiFi Kits for bisulfite sequencing
KAPA CODE | ROCHE CAT NO | DESCRIPTION | KIT SIZE |
---|---|---|---|
KK2801 | 7959052001 | KAPA HiFi HotStart Uracil+ Kit | 50 rxn |
KK2802 | 7959079001 | KAPA HiFi HotStart Uracil+ Kit | 250 rxn |
KAPA HiFi Library Amplification Kits
View Full TableKAPA HiFi Library Amplification Kits
KAPA Code |
Roche Cat. No |
Description |
Kit Size |
How to buy |
---|---|---|---|---|
KK2611 |
07958951001 |
HotStart Ready Mix without primer mix | 50 x 50 µL rxn |
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KK2612 |
07958960001 |
HotStart Ready Mix without primer mix | 250 x 50 µL rxn |
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KK2620 |
07958978001 |
HotStart ReadyMix with primer mix | 50 x 50 µL rxn |
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KK2621 |
07958986001 |
HotStart ReadyMix with primer mix | 250 x 50 µL rxn |
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KK2702 |
07959028001 |
HotStart ReadyMix real-time PCR library amplification, with fluorescent standards | 250 x 50 µL rxn |
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For purchasing or general questions
For online ordering