KAPA HiFi Uracil+ Kit

For Research Use Only. Not for use in diagnostic procedures.

KAPA HiFi Uracil+ Kit

Overview
 

KAPA HiFi Uracil+ DNA Polymerase is a modified version of KAPA HiFi DNA Polymerase that is engineered to tolerate uracil residues in bisulfite-treated DNA. Bisulfite treatment of DNA results in the conversion of unmethylated cytosine residues into uracil, while the methylated residues are left unmodified. Enzymes used for library amplification in bisulfite-sequencing workflows must be able to read through uracil residues and tolerate low concentrations of AT-rich DNA. Traditional high-fidelity DNA polymerases are typically not suitable for this type of application as the enzymes stall when a uracil is encountered. KAPA HiFi Uracil+ DNA Polymerase has been developed to read through uracil residues while still retaining the performance benefits of HiFi DNA Polymerase, such as high yields, low-bias, and uniform sequencing coverage.

Benefits of KAPA HiFi Uracil+ Kits
  • Higher yields and reduced size bias for templates containing  uracil compared to other methods
  • More uniform bisulfite-sequencing coverage and improved  representation of AT-rich sequences compared to competitor kits

Product highlights

Amplification of uracil-containing template
  • Effective amplification through uracil and other modified residues

 

View data on KAPA HiFi Uracil showing no inhibition by dUTP.

High yields of low-bias bisulfite-converted library generation
  • Reduced cycles and lower PCR duplicates through high-efficiency amplification
  • Improved representation of all library fragments and sequence regions due to low amplification bias
     

View comparative data showing higher yield and less size bias using KAPA HiFi Uracil+ compared to Agilent Pfu Turbo Cx.

Greater coverage depth uniformity

See data on even coverage with whole genome bisulfite (WGBS) libraries using KAPA HiFi Uracil+.

Improved representation of AT-rich sequences
  • Greater tolerance to AT-rich regions
  • Improved bisulfite-sequencing read quality

 

See data showing better representation of AT-rich sequences using KAPA HiFi Uracil+ compared to Agilent Pfu Turbo Cx.

Efficient target enrichment of bisulfite-treated DNA

View data showing efficient amplification of bisulfite-treated DNA by KAPA HiFi Uracil after target enrichment.

 

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*Data on file.

 

Store kits for 12 months at -20°C.

KAPA HiFi HotStart Uracil+ ReadyMix (2X) contains DNA Polymerase, reaction buffer, dNTPs and MgCl2 (at a final concentration of 2.5 mM).

Spec
Description
Starting Material
DNA, cDNA, plasmid DNA, bisulfite-treated DNA
Input Amount
1 - 100 ng for genomic DNA; 10 pg - 1 ng for less-complex DNA

For purchasing or general questions

For online ordering

KAPA HiFi HotStart Uracil+ ReadyMix (2X) contains DNA Polymerase, reaction buffer, dNTPs and MgCl2 (at a final concentration of 2.5 mM)

KAPA Code
Roche Cat. No
Description
Kit Size
How to buy
KK2801
07959052001
50 x 50 µL reactions 1.25 mL
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KK2802
07959079001
250 x 50 µL reactions 6.25 mL
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For purchasing or general questions

For online ordering