KAPA HiFi Uracil+ Kit

Figure 1. KAPA HiFi Uracil+ is not inhibited by dUTP. SYBR Green real-time PCR was used to monitor amplification of a 452 bp amplicon over ten-fold dilutions (80 pM – 0.8 fM) with or without 0.2 mM dUTP. KAPA HiFi DNA Polymerase shows typical inhibition by uracil, while KAPA HiFi Uracil+ DNA Polymerase shows no amplification inhibition.

Figure 3. KAPA HiFi Uracil+ provides higher yields and minimal size bias. Human whole genome bisulfite-treated libraries were amplified using standard protocols with 12, 14, or 16 cycles and the amplified libraries were analyzed using a Bioanalyzer 2100 High Sensitivity DNA chip. When compared with Agilent Pfu Turbo Cx, KAPA HiFi Uracil+ DNA Polymerase produced much higher yields (left) with very little size bias (right)

Figure 5. KAPA HiFi Uracil+ DNA Polymerase provides improved representation of AT-rich sequences compared to Agilent Pfu Turbo Cx kit. 100 ng of bisulfite-treated library DNA was amplified (8 cycles) with KAPA HiFi Uracil+ DNA Polymerase or Agilent Pfu Turbo Cx.

Figure 6. More even coverage with WGBS libraries. Replicate WGBS libraries were prepared from parasite gDNA, bisulfite-treated, and amplified using KAPA HiFi Uracil+ DNA Polymerase or Agilent Pfu Turbo Cx.

Figure 7. Identification of differentially methylated regions using the SeqCap Epi Target Enrichment System. Libraries were prepared from Burkitt’s lymphoma cell line (NA04671) or normal control cell line (CEPH; NA12762) DNA with the KAPA HTP Library Preparation Kit, and subjected to bisulfite-treatment after pre-capture amplification. Capture was performed with a 3.2 Mb SeqCap Epi Choice design, and amplified with KAPA HiFi Uracil+. Differential methylation was observed by the large increase in percent methylation in the lymphoma vs. normal cell line DNA.