KAPA HyperCap Oncology Panel

For Research Use Only. Not for use in diagnostic procedures.

KAPA HyperCap Oncology panel

Leveraging more than a decade of probe design experience with an improved probe manufacturing process, the KAPA HyperCap Oncology Panel combines targeted interrogation of genes associated with somatic oncology with greater sequencing efficiency. Order this 314 Kb hybrid capture panel today and start investigating genes associated with oncology tomorrow.

  • Capture 13 genes strongly associated with somatic oncology, along with additional hotspot variants commonly included in commercial FFPET and cfDNA controls
  • Access valuable, often elusive information from low-quality FFPET samples
  • Achieve >97.5% of target coverage at 1000X from as little as 10 ng cfDNA with (10 M high-quality clusters)
  • Streamline targeted sequencing with our HyperCap Workflow v3, driven by KAPA HyperPrep or KAPA HyperPlus Library Prep Kits
Gene List, Full CDS Coverage
ATM KRAS
BRCA1 MET
BRCA2 MYCN
BRIP1 RAD51C
CHEK2 RAD51D
EGFR TP53
ERBB2  

Access valuable, often elusive information from low-quality FFPET samples

  • Unlock the potential of challenging samples with the optimized KAPA HyperCap FFPET DNA workflow and the molecular specificity provided by KAPA Universal UMI Adapters
  • Improve sequencing accuracy with low-quantity and low-quality FFPET DNA samples by achieving a high percentage of error-free positions across your target (>94%) 
  • Achieve uniform and deep sequencing coverage with >92% of bases covered within 0.5X–2X of the median and >88% of exon bases covered at >300X
key sequencing and enrichment metrics

Figure 1. Key sequencing metrics with the KAPA HyperCap Oncology Panel. Two real-world low-quality FFPET DNA samples were compared with the Horizon™ HD789 control FFPE DNA sample. Sample libraries (in duplicates) were prepared following the KAPA HyperCap FFPET DNA Workflow from 10 ng FFPE DNA with the KAPA HyperPlus Kit. Following single library hybridization and capture, the final libraries were sequenced on a NextSeq™ 550 system and downsampled to 10 M HQ filtered clusters (2 x 150 bp) prior to analysis. 

Achieve >97.5% of target coverage at 1000X from as little as 10 ng cfDNA

  • Attain high genome equivalent recovery rates and cover >97.5% of your target bases with at least 1000X from just 10 ng of cfDNA with the KAPA HyperCap cfDNA Workflow
  • Preserve sample complexity by retrieving the sequence of unique input molecules with KAPA Universal UMI Adapters
  • Recover a high percentage of duplex molecule sequences for deep, efficient cell-free DNA sequencing with KAPA Universal UMI Adapters
key sequencing and enrichment metrics

Figure 2: Key sequencing metrics for real-world and control cfDNA samples using the KAPA HyperCap Oncology Panel. Libraries were prepared from five cfDNA samples from healthy donors in parallel with SeraSeq® ctDNA Complete™ Reference Material AF0.5%; libraries were prepared in duplicate from 10 ng of DNA. The KAPA HyperCap cfDNA workflow was followed using the KAPA HyperPrep Kit and single-plex probe hybridization and capture. Final libraries were sequenced on a NovaSeq™ 6000 system (2 x 150 bp). Reads were downsampled to 50 M high-quality filtered clusters per sample. Light blue bars represent the average of all cfDNA samples. Error bars represent standard deviation.

Project: KAPA HyperCap Fixed Panels, Pleasanton, CA 2021

hypercap workflow hypercap oncology

Streamline target enrichment with the KAPA HyperCap Workflow v3

KAPA HyperCap Workflow v3 delivers complex libraries by combining the high conversion rate of KAPA HyperPrep or KAPA HyperPlus Kits with KAPA Target Enrichment, creating a streamlined, single-vendor supported workflow.

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kapa te probes hypercap oncology

Explore KAPA Target Enrichment Probes: Better by Design

KAPA Target Enrichment Probes are manufactured with KAPA HiFi DNA Polymerase and demonstrate extremely high sequence fidelity, high on-target rates, and high accuracy and reproducibility.

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Ordering information for KAPA HyperCap Oncology Panel

Roche Catalog# Roche Cat No. Description Kit Size Kit size (Reaction)
09462384001 09462384001 KAPA Oncology panel 12 rxn 12
09462457001 09462384001 KAPA Oncology panel 24 rxn 24
09462465001 09462465001 KAPA Oncology panel 48 rxn 48

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For online ordering