KAPA HyperCap Oncology Panel

Figure 1. Key sequencing metrics with the KAPA HyperCap Oncology Panel. Two real-world low-quality FFPET DNA samples were compared with the Horizon™ HD789 control FFPE DNA sample. Sample libraries (in duplicates) were prepared following the KAPA HyperCap FFPET DNA Workflow from 10 ng FFPE DNA with the KAPA HyperPlus Kit. Following single library hybridization and capture, the final libraries were sequenced on a NextSeq™ 550 system and downsampled to 10 M HQ filtered clusters (2 x 150 bp) prior to analysis. 

Figure 2: Key sequencing metrics for real-world and control cfDNA samples using the KAPA HyperCap Oncology Panel. Libraries were prepared from five cfDNA samples from healthy donors in parallel with SeraSeq® ctDNA Complete™ Reference Material AF0.5%; libraries were prepared in duplicate from 10 ng of DNA. The KAPA HyperCap cfDNA workflow was followed using the KAPA HyperPrep Kit and single-plex probe hybridization and capture. Final libraries were sequenced on a NovaSeq™ 6000 system (2 x 150 bp). Reads were downsampled to 50 M high-quality filtered clusters per sample. Light blue bars represent the average of all cfDNA samples. Error bars represent standard deviation.

Project: KAPA HyperCap Fixed Panels, Pleasanton, CA 2021