Unleash the true power of digital PCR
It's time for a leap forward in digital PCR technology.
The Roche Digital LightCycler® System is the digital PCR instrument of tomorrow. With a unique combination of 3 nanowell plate configurations, 6 advanced optical channels, and 5x concentrated DNA and RNA master mixes, it has the potential to help your lab to make the leap from publishing research to producing clinically viable assays.
The unique hexagonal-shaped nanowell partitions and the filling dye that validates partitioning gives the Digital LightCycler® System the ability to handle a wide variety of extracted samples such as FFPET, cfDNA, Whole Blood, and more.
Through these partitions, target sequences are efficiently concentrated within isolated microreactors. This concentration effect reduces template competition, thereby enabling the detection of rare mutations and allowing for a higher tolerance to inhibitors present in a sample.
15μL, 100,000 partitions
Copy Number Variation
Human Genetic Disease
30μL, 28,000 partitions
Gene Expression
Transplant Rejection
45μL, 20,000 partitions
Cell-free DNA
Oncology
Rare Mutation Detection
6 advanced optical channels enable a high degree of multiplexing for DNA or RNA targets.
Get the most out of your samples with the Digital LightCycler® System's 5x concentrated master mixes.
* Roche data on file: DH_02365.01_031B_Digital_LightCycler_Reagent_Feasibility_Report_v3,
Document Number: 0000000000001200000501942
Roche data on file: DH-02365.01-500E_Digital LightCycler System Performance report
Roche data on file: DHF Digital_LightCycler_Reagent_Feasibility_Report
Roche data on file: DH-02365.01-008B_Digital_LightCycler_System_Feasibility_Report
Sample utilization vs. competitive system1-4
References
Experience sensitivity, precision, flexibility, and integration in one powerful
clinical research tool. The Digital LightCycler® System from Roche is a
digital PCR system that can help laboratories push forward the boundaries
of clinical research and has the potential to advance global medical knowledge.
Digital PCR (dPCR) is a relatively new PCR-based method for quantification of DNA and RNA targets. It builds on traditional PCR amplification methods to provide precise, absolute quantification of nucleic acids with single molecular resolution.
Key advantages of digital PCR:
This presentation from AACR 2024 highlights two different oncology research applications for Roche’s Digital LightCycler® dPCR System. First, Yu Zhao, Roche Sr. Applications Specialist, describes the DNA methylation analysis of the RASSF1A promoter in cancer cell lines; this assay enables precise detection and absolute quantification without requiring bisulfite conversion or reference standards. Then, GT Molecular discusses a new AML research panel developed for the Digital LightCycler®; this assay enables sensitive detection of AML-associated variants using DNA extracted from liquid biopsy samples. The assay highlights and leverages the clinical utility of the Digital LightCycler® dPCR System.
Presenter: Simran Saini, PhD
Principal Scientist II, Sequencing Workflows, Applications and Technology, Roche
In this presentation, we discuss the Digital LightCycler® System, a digital system for DNA and RNA with 6-color capability for flexible, multiplexed biomarker detection. With advances in sensitivity, precision, flexibility, and integration, the Digital LightCycler® System is a powerful digital PCR system that aims to help laboratories push the boundaries of clinical research forward. Three configurations of consumables are available to meet varied application needs, including high sample volume for cfDNA screening or greater number of partitions for high-resolution CNV analysis. We present data that demonstrates the sensitive performance of the Digital LightCycler® System as the powerful new addition to the Roche PCR ecosystem.
| Roche Cat. No |
Description |
|---|---|
| 09274804001 | Digital LightCycler® Analyzer |
| 09224777001 | Digital LightCycler® Partioning Engine |
| 09393544001 | Digital LightCycler® DNA Master Kit |
| 08897115001 | Digital LightCycler® RNA Master Kit |
| 09033696001 | Digital LightCycler® Universal Plate |
| 09033700001 | Digital LightCycler® HiRes Plate |
| 09033718001 | Digital LightCycler® HiSens Plate |
| 08897115001 | Digital LightCycler® Partioning Fluid |
