The KAPA Stranded RNA-Seq Kits are the first-generation KAPA RNA library preparation kits that combine the use of a “with-bead” protocol with KAPA HiFi DNA Polymerase selected through our directed evolution technology for high efficiency, high fidelity and low-bias amplification to provide quality RNA libraries with flexible input amounts.
For single-day single-tube streamlined RNA library preparation, we recommend the KAPA RNA HyperPrep Kits.
Roche’s NGS Automation Support Team, in collaboration with non-Roche liquid handling vendors, creates menus of automated methods for KAPA library prep reagents.
Let’s talk about the next steps to get you up and running with automating your NGS library prep protocols. Visit support for non-Roche liquid handlers to learn more.
| Product Name | KAPA Stranded RNA-seq Kits | KAPA Stranded mRNA-seq Kits | KAPA Stranded RNA-seq Kits with RiboErase (HMR) |
|---|---|---|---|
| Library Preparation Duration | 6-8 hours | 8-10 hours | 10-12 hours |
| Sample Input Amount | 10-400 ng into library prep | 100 ng - 4 µg into mRNA capture | 100 ng - 1 µg into rRNA depletion |
| Sample Types | High-quality total RNA Degraded or FFPE total RNA Poly(A)-selected and/or depleted RNA |
High-quality total RNA | High-quality total RNA Degraded or FFPE total RNA |
| Species | Eukaryotic (Animal, Plant, etc.) Prokaryotic (Bacteria, etc.) |
Eukaryotic (Animal, Plant, etc.) | Human, Mouse, and Rat Other species can be supported via KAPA RiboDesigner custom rRNA depletion |
| Stranded RNA-sequencing | Yes | Yes | Yes |
| Amplification |
Compatible with low bias amplification |
Compatible with low bias amplification |
Compatible with low bias amplification |
| Reaction Purification | KAPA Pure Beads are not included | KAPA Pure Beads are not included | KAPA Pure Beads are not included |
| Multiplexing | Yes. KAPA Adapters are not supplied, but available separately | Yes. KAPA Adapters are not supplied, but available separately | Yes. KAPA Adapters are not supplied, but available separately |
| Applications | Whole transcriptome sequencing Gene expression with target enrichment Splice junction and gene fusions with target enrichment |
Gene expression Annotation Splice junction and gene fusions |
Whole transcriptome Long non-coding RNA Annotation Gene expression Splice junction and gene fusions |
| Automation Friendly | Yes. Demonstrated and verified on many liquid handling systems. Learn more |
Yes. Demonstrated and verified on many liquid handling systems. Learn more |
Yes. Demonstrated and verified on many liquid handling systems. Learn more |
| Genialis™ Data Analysis and Visualization Solution | No | No | No |
| KAPA RiboDesigner Custom rRNA Depletion Support | N/A | N/A | Yes |
| Compatible Sequencing Platforms | Illumina Sequencers | Illumina Sequencers | Illumina Sequencers |
| Storage Information | Kits can be stored for up to 8 months at -20°C |
Kits can be stored for up to 8 months at -20°C KAPA mRNA Capture Beads can be stored for up to 8 months at 4˚C |
Kits can be stored for up to 8 months at |
| KAPA Code |
Roche Cat. No |
Description |
Kit Size |
|---|---|---|---|
| KK8400 |
07962142001 |
Stranded RNA-Seq Kit | 24 libraries |
| KK8401 |
07962169001 |
Stranded RNA-Seq Kit | 96 libraries |
| KK8420 |
07962193001 |
KAPA Stranded mRNA-Seq Kit, with KAPA mRNA Capture Beads | 24 libraries |
| KK8421 |
07962207001 |
KAPA Stranded mRNA-Seq Kit, with KAPA mRNA Capture Beads | 96 libraries |
| KK8483 |
07962282001 |
KAPA Stranded RNA-Seq Kit with RiboErase (HMR) | 24 reactions |
| KK8484 |
07962304001 |
KAPA Stranded RNA-Seq Kit with RiboErase (HMR) | 96 reactions |
| KAPA Code |
Roche Cat. No |
Description |
Kit Size |
|---|---|---|---|
| KK8000 |
07983271001 |
KAPA Pure Beads (5 mL) | 5 mL |
| KK8001 |
07983280001 |
KAPA Pure Beads (30 mL) | 30 mL |
| KK8002 |
07983298001 |
KAPA Pure Beads (60 mL) | 60 mL |
| KK8721 |
08278539001 |
KAPA Adapter Dilution Buffer (25 mL) | 25 mL |
| KK8727* |
08861919702 |
KAPA Unique Dual-Indexed Adapter Kit, (15 µM) |
96 adapters x 20 µl each |
