KAPA Stranded RNA-Seq Kits

For Research Use Only. Not for use in diagnostic procedures.

KAPA Stranded RNA-Seq Kit
Overview

The KAPA Stranded RNA-Seq Kits are the first-generation KAPA RNA library preparation kits that combine the use of a “with-bead” protocol with KAPA HiFi DNA Polymerase selected through our directed evolution technology for high efficiency, high fidelity and low-bias amplification to provide quality RNA libraries with flexible input amounts.

For single-day single-tube streamlined RNA library preparation, we recommend the KAPA RNA HyperPrep Kits.

For purchasing or general questions

For online ordering

KAPA Stranded RNA-seq Kits Specifications and Comparison

Product Name KAPA Stranded RNA-seq Kits KAPA Stranded mRNA-seq Kits KAPA Stranded RNA-seq Kits with RiboErase (HMR)
Library Preparation Duration 6-8 hours 8-10 hours 10-12 hours
Sample Input Amount 10-400 ng into library prep 100 ng - 4 µg into mRNA capture 100 ng - 1 µg into rRNA depletion
Sample Types

High-quality total RNA


Degraded or FFPE total RNA


Poly(A)-selected and/or depleted RNA

High-quality total RNA

High-quality total RNA


Degraded or FFPE total RNA

Species

Eukaryotic (Animal, Plant, etc.)


Prokaryotic (Bacteria, etc.)

Eukaryotic (Animal, Plant, etc.)

Human, Mouse, and Rat


Other species can be supported via KAPA RiboDesigner custom rRNA depletion

Stranded RNA-sequencing Yes Yes Yes
Amplification

KAPA HiFi DNA Polymerase


Compatible with low bias amplification

KAPA HiFi DNA Polymerase


Compatible with low bias amplification

KAPA HiFi DNA Polymerase


Compatible with low bias amplification

Reaction Purification KAPA Pure Beads are not included KAPA Pure Beads are not included KAPA Pure Beads are not included
Multiplexing Yes.  KAPA Adapters are not supplied, but available separately Yes.  KAPA Adapters are not supplied, but available separately Yes.  KAPA Adapters are not supplied, but available separately
Applications

Whole transcriptome sequencing


Gene expression with target enrichment


Splice junction and gene fusions with target enrichment

Gene expression


Annotation


Splice junction and gene fusions

Whole transcriptome


Long non-coding RNA Annotation


Gene expression


Splice junction and gene fusions

Automation Friendly Yes.  Demonstrated and verified on many liquid handling systems.
Learn more
Yes.  Demonstrated and verified on many liquid handling systems.
Learn more
Yes.  Demonstrated and verified on many liquid handling systems.
Learn more
Genialis™ Data Analysis and Visualization Solution No No No
KAPA RiboDesigner Custom rRNA Depletion Support N/A N/A Yes   Learn more
Compatible Sequencing Platforms Illumina Sequencers Illumina Sequencers Illumina Sequencers
Storage Information

Kits can be stored for up to 8 months at -20°C

Kits can be stored for up to 8 months at -20°C


KAPA mRNA Capture Beads can be stored for up to 8 months at 4˚C

Kits can be stored for up to 8 months at 

For purchasing or general questions

For online ordering

KAPA Code
Roche Cat. No
Description
Kit Size
How to buy
KK8400
07962142001
Stranded RNA-Seq Kit 24 libraries
Login for pricing
KK8401
07962169001
Stranded RNA-Seq Kit 96 libraries
Login for pricing
KK8420
07962193001
KAPA Stranded mRNA-Seq Kit, with KAPA mRNA Capture Beads 24 libraries
Login for pricing
KK8421
07962207001
KAPA Stranded mRNA-Seq Kit, with KAPA mRNA Capture Beads 96 libraries
Login for pricing
KK8483
07962282001
KAPA Stranded RNA-Seq Kit with RiboErase (HMR) 24 reactions
Login for pricing
KK8484
07962304001
KAPA Stranded RNA-Seq Kit with RiboErase (HMR) 96 reactions
Login for pricing
KAPA Stranded RNA-seq Kits include reagents for RNA fragmentation, 1st strand cDNA synthesis, 2nd strand synthesis/marking, and cDNA library preparation, including A-tailing, ligation, and library amplification.
KAPA Code
Roche Cat. No
Description
Kit Size
How to buy
KK8000
07983271001
KAPA Pure Beads (5 mL) 5 mL
Login for pricing
KK8001
07983280001
KAPA Pure Beads (30 mL) 30 mL
Login for pricing
KK8002
07983298001
KAPA Pure Beads (60 mL) 60 mL
Login for pricing
KK8721
08278539001
KAPA Adapter Dilution Buffer (25 mL) 25 mL
Login for pricing
KK8727*
08861919702

KAPA Unique Dual-Indexed Adapter Kit, (15 µM)

96 adapters x 20 µl each
Login for pricing
*Contains KAPA Adapter Dilution Buffer, as well as three additional sealing films to support multiple use

For purchasing or general questions

For online ordering