For research use only. Not for use in diagnostic procedure.
KAPA HyperExome V2 Probes, the latest Roche WES design, delivers superior coverage of recent versions of ACMGv3.1, RefSeq, CCDS, ClinVar, Ensembl, and COSMIC genomic databases. This design also reduces sequencing requirements, with a compact capture target of 43.2 Mb covering 37.5 Mb of key content.
Figure 1. Superior probe database coverage by the KAPA HyperExome V2 design compared to other suppliers’ designs across important genomic databases (better coverage by as much as 17%). Database data retrieved in Jan 2023, unpadded capture target used to compare across suppliers. The KAPA HyperExome V2 panel was designed to cover coding exon sequence from the following annotation sources: RefSeq (June 29, 2022), ClinVar (June 29, 2022) and Ensembl release v106.3
Figure 2. Percent bases covered by at least 30X across some key genomic databases. KAPA HyperExome V2 Probes deliver better database coverage compared to Suppliers I, A, and T, which leave more “blind” spots and may thus require repeated experiments and/or the addition of supplemental probes. Supplier sample prep protocols were followed with singleplex 4h hybridizations for Supplier I (3 replicates, NA12878), 8-plex O/N hybridizations for Supplier A (48 data points from 6 replicate captures of 16 coriell DNAs), 8-plex O/N hybridizations for Supplier T (72 data points from 16 coriell and 24 blood-extracted DNAs) and 16-plex O/N hybridizations for the KAPA HyperExome V2 Probes (48 data points from 16 blood-extracted DNAs in 3 captures) with the KAPA EvoPlus Kit, KAPA HyperPlus Kit, and KAPA HyperPrep Kit (48 data points from 16 coriell DNAs in 3 captures). KAPA HyperExome V2 Probes-enriched libraries were sequenced on a NovaSeq™ 6000 System at 2 x 100 bp and 60 M high-quality reads were analyzed per library. Supplier-enriched libraries were sequenced proportionally to their capture target size.3
Figure 3. Exceptional uniformity of normalized capture coverage across the extremes of the GC% spectrum. The blue dashed line represents the optimal uniformity in the ideal state in which all regions—regardless of their GC content—would be equally covered. Exome libraries were prepared using KAPA HyperExome V2 Probes, Supplier T exome probes, or Supplier A exome probes as described in Figure 2. Sequencing and analysis were carried out as described for Figure 2.
Increase result confidence by following sample identities throughout the workflow with sample-tracking endogenous SNPs covered by the KAPA HyperExome V2 Probes. Or, explore new potential capabilities with a unique set of 96 probes that are included in the panel and designed to capture non-naturally occurring sequences that can be spiked into samples.
KAPA HyperCap Workflow delivers complex libraries by combining the high conversion rate of KAPA HyperPrep, KAPA HyperPlus, and KAPA EvoPlus Kits with KAPA HyperCap probes (such as KAPA HyperExome V2 Probes), creating a streamlined, single-vendor supported workflow.
The design and annotation files provide information about genomic regions covered by the capture probes and the genes included in these regions.
The KAPA HyperExome V2 panel was designed to cover coding exon sequence from the following annotation sources: RefSeq (June 29, 2022), ClinVar (June 29, 2022) and Ensembl release v106. The panel covers 529 sample tracking SNPs to facilitate sample identity tracking in the workflow. A set of 96 probes, composed of non-naturally occurring sequences, are included in the design. These probes may be used to capture exogenous synthetic DNA fragments, potentially used as process controls.
The total design size is 43.2 Mb of capture target space; the capture targets BED file shows the capture probe footprint. The UCSC Browser BED files are provided for upload as a custom track. All design files are provided in the original hg38 genome assembly and also converted to hg19 genome assembly. Conversion was performed using the NCBI Genome Remapping Service.
Download hg38 Design Files for the KAPA HyperExome V2 Probes
Download hg19 Design Files for the KAPA HyperExome V2 Probes
*Databases reviewed June 2022
1. A SNP profiling panel for sample tracking in whole-exome sequencing studies. Pengelly RJ, et al. Genome Med. 2013 Sep 27;5(9):89. doi: 10.1186/gm492. eCollection 2013
2. A SNP panel for identification of DNA and RNA specimens. Yousefi S, et al. BMC Genomics. 2018 Jan 25;19(1):90. doi: 10.1186/s12864-018-4482-7.
3. Project: Roche KAPA HyperExome V2 Launch Data, Pleasanton, CA, Mar. 2023
For research use only. Not for use in diagnostic procedure.
Demo data files are available. Please contact a local Roche representative.
Demo data files are available. Please contact a local Roche representative.
| Product Name |
Capture reactions |
Catalog No. |
|---|---|---|
| KAPA HyperExome V2 Prep Kit Complete KAPA HyperCap Workflow NGS sample prep kit for 192 8-plex reaction samples Includes: KAPA HyperPrep Kit, Universal Adapters, HyperBeads, Probes Resuspension Buffer, HyperExome V2 Probes, HyperCapture Reagent Kit, and Hypercapture Bead Kit |
192 8-plex rxn | 9983759001 |
| KAPA HyperExome V2 Plus Kit Complete KAPA HyperCap Workflow NGS sample prep kit for 192 8-plex reaction samples Includes: KAPA HyperPlus Kit, Universal Adapters, HyperBeads, Probes Resuspension Buffer, HyperExome V2 Probes, HyperCapture Reagent Kit, and Hypercapture Bead Kit |
192 8-plex rxn | 9983775001 |
| KAPA HyperExome V2 Evo Kit Complete KAPA HyperCap Workflow NGS sample prep kit for 192 8-plex reaction samples Includes: KAPA EvoPlus Kit, Universal Adapters, HyperBeads, Probes Resuspension Buffer, HyperExome V2 Probes, HyperCapture Reagent Kit, and Hypercapture Bead Kit |
192 8-plex rxn | 9983783001 |
| KAPA HyperExome V2 Probes | 12 | 9718630001 |
| 24 | 9718648001 | |
| 48 | 9718656001 | |
| 96 | 9718664001 | |
| 192 | 9718672001 | |
| 384 | 9718699001 | |
| 768 | 9718702001 | |
| 1152 | 9718729001 | |
| 1536 | 9718737001 |
For research use only. Not for use in diagnostic procedure.
