Simplify your NGS library prep with the AVENIO Edge System, a fully-automated IVD liquid handler for an end-to-end library preparation, target enrichment, quantification, normalization and pooling.
For more information, contact us.
NGS library prep workflows are labor-intensive and time-consuming—especially if they include target enrichment (with oncology panels, for example). As a result, many genomics laboratories now rely on automated library preparation to streamline processes, reduce user-to-user variability, and improve reproducibility.
However, many of the automated liquid handlers on the market are cumbersome to program, set up, and run. Now, with the AVENIO Edge Liquid Handling System, labs that have no prior experience with NGS or automation can easily run complex library prep workflows—freeing up hours of time for other projects and reducing errors that can occur with manual methods.
Queen's University Belfast shares how AVENIO Edge System got their ACTIONED Project on track.
"Using the Avenio Edge System we can free up team members to work in different parts of the project, or even take [on] new projects that bring additional value. The AVENIO Edge System has played a vital role in making sure that we can keep our TAT in check." Prof. David Gonzalez de Castro, PhD FRCPath
"The amount of the ctDNA that we have for each sample is very small. We therefore have only one opportunity to run the ctDNA on a library prep. With the AVENIO Edge we have the confidence and reliability that we can run ctDNA samples and [know] it will work." Peter Stewart, PhD
Watch this ~2 min video to learn about the capabilities and specifications of the AVENIO Edge Liquid Handler for NGS library prep and target enrichment. The AVENIO Edge provides an end-to-end library prep workflow, including cleanup steps, quantification, normalization, and pooling.
Watch this video to learn how the AVENIO Edge IVD Liquid Handling System helps make it easier for molecular pathology labs to prepare next-generation sequencing (NGS) libraries for applications such as cancer research, exome sequencing, and beyond.
Watch Dr. Christien Betz describe how his lab uses KAPA HyperExome V2 and the AVENIO Edge Liquid Handling System to solve their whole-exome sequencing bottlenecks. Dr. Betz gave this presentation at the 2024 European Society of Human Genetics (ESHG) conference.
Here is a growing menu of workflows currently available on the system:
Here is a growing menu of workflows currently available on the system:
| Workflow | Maximum samples (Per Run) | Estimated setup time (minutes) |
|---|---|---|
| AVENIO Edge HyperPrep or AVENIO HyperPlus DNA library preparation* | 48 | 20 |
| AVENIO Edge Target Enrichment with KAPA HyperExome or custom panels** | 48 | 20 |
| AVENIO Edge HyperCap DNA library preparation and target enrichment* | 24 | 20 |
| Input DNA quantification, normalization and buffer exchange | 96 | 15 |
| Post-capture pooling and quantification | 48 | 15 |
| *These workflows use UDI primers and universal UMI or non-UMI adapters. **The input is indexed DNA or cDNA libraries |
||
9. Cooling unit
10. Tube racks
11. Piercing tool
12. Reagent mini racks
13. Reagent drawer
14. P1000, P200, P50 Filtered tips
15. Quantification plate station
16. Consumable drawer
17. Gripper fingers exchange station
18. Quantification module
19. 2D Barcode reader
20. Processing plates
21. Processing plate lids
Roche’s NGS Automation Support Team, in collaboration with non-Roche liquid handling vendors, creates menus of automated methods for KAPA library prep reagents. Let’s talk about the next steps to get you up and running with automating your NGS library prep protocols. Visit support for non-Roche liquid handlers to learn more.
AVENIO Edge reagents and workflows are for Research Use Only. They are not intended for diagnostic applications.
| Roche Cat. No. |
Description |
|
|---|---|---|
| 09283234001 | AVENIO Edge Instrument | |
| 09424962001 | AVENIO Designer Software - Annual Subscription |
| Roche Cat. No. |
Description |
Kit Size |
|---|---|---|
| 09200860001 | AVENIO Edge DNA HyperPlus Kit | 3 x 8 reactions |
| 09200878001 | AVENIO Edge DNA HyperPlus Kit | 3 x 24 reactions |
| 09200886001 | AVENIO Edge DNA HyperPrep Kit | 3 x 8 reactions |
| 09200894001 | AVENIO Edge DNA HyperPrep Kit | 3 x 24 reactions |
| 09200908001 | AVENIO Edge Target Enrichment, A | 3 x 8 reactions |
| 09200916001 | AVENIO Edge Target Enrichment, A | 3 x 24 reactions |
| 09200924001 | AVENIO Edge Target Enrichment, B | 3 sample runs |
| 09200959001 | AVENIO Edge UDI Primer Mixes Plate | 96 wells of primer mixes |
| 09200967001 | AVENIO Edge HyperPure Beads | 3 sample runs |
| 09200975001 | AVENIO Edge HyperPlex UMI Adapter | 3 x 8 reactions |
| 09200983001 | AVENIO Edge HyperPlex UMI Adapter | 3 x 24 reactions |
| 09200991001 | AVENIO Edge HyperPlex Adapters | 3 x 8 reactions |
| 09201009001 | AVENIO Edge HyperPlex Adapters | 3 x 24 reactions |
| 09200851001 | AVENIO Edge Quantification Kit | 3 sample runs |
| 09314512001 | AVENIO Edge Solution A Kit | 3 sample runs |
| 09496386001 | AVENIO Edge HyperPrep with UMI, 8 reactions Library Prep and Target Enrichement | 3 x 8 reactions, 3 complete KAPA HyperCap runs |
| 09496394001 | AVENIO Edge HyperPrep without UMI, 8 reaction Library Prep and Target Enrichement | 3 x 8 reactions, 3 complete KAPA HyperCap runs |
| 09496408001 | AVENIO Edge HyperPlus with UMI, 8 reaction Library Prep and Target Enrichement | 3 x 8 reactions, 3 complete KAPA HyperCap runs |
| 09496416001 | AVENIO Edge HyperPlus without UMI, 8 reaction Library Prep and Target Enrichement | 3 x 8 reactions, 3 complete KAPA HyperCap runs |
| 09496424001 | AVENIO Edge HyperPrep with UMI, 24 reactions Library Prep and Target Enrichement | 3 x 24 reactions, 3 complete KAPA HyperCap runs |
| 09496432001 | AVENIO Edge HyperPrep without UMI 24 reactions Library Prep and Target Enrichement | 3 x 24 reactions, 3 complete KAPA HyperCap runs |
| 09496459001 | AVENIO Edge HyperPlus with UMI, 24 reactions Library Prep and Target Enrichement | 3 x 24 reactions, 3 complete KAPA HyperCap runs |
| 09496467001 | AVENIO Edge HyperPlus without UMI 24 reactions Library Prep and Target Enrichement | 3 x 24 reactions, 3 complete KAPA HyperCap runs |
| Roche Cat. No. |
Description |
Package Size |
|---|---|---|
| 09056351001 | AVENIO Edge Tip Park | 16 tip parks |
| 08994609001 | AVENIO Edge Processing Plate | 50 plates |
| 09030956001 | AVENIO Edge Quantification Plate | 50 plates |
| 09031022001 | AVENIO Edge Processing Plate Lid | 50 lids |
| 09031049001 | AVENIO Edge Liquid Waste Plate | 60 plates |
| 09164081001 | AVENIO Edge Tube, Panel 1 | 100 tubes |
| 09164090001 | AVENIO Edge Tube, Panel 2 | 100 tubes |
| 09164103001 | AVENIO Edge Tube, Panel 3 | 100 tubes |
| 09164111001 | AVENIO Edge Tube, Panel 4 | 100 tubes |
| 09313630001 | AVENIO Edge Pooling Tube | 100 tubes |
| 09336320001 | AVENIO Edge Pipette Tip, 50 uL | 24 boxes of 96 tips (total of 2304 tips) |
| 09336346001 | AVENIO Edge Pipette Tip, 200 uL | 24 boxes of 96 tips (total of 2304 tips) |
| 09336354001 | AVENIO Edge Pipette Tip, 1000 uL | 24 boxes of 96 tips (total of 2304 tips) |
AVENIO Edge reagents and workflows are for Research Use Only. They are not intended for diagnostic applications.
AVENIO Edge reagents and workflows are for Research Use Only. They are not intended for diagnostic applications.
