Streamline NGS sample prep with KAPA HyperCap Workflow v3

KAPA HyperCap Workflow v3 produces high-quality, sequencing-ready libraries in less than two days by combining the high conversion rate of KAPA HyperPrep or KAPA HyperPlus Kits, with efficient, uniform KAPA Target Enrichment probes in a streamlined, single-vendor supported workflow. KAPA Universal Adapters and KAPA UDI primer mixes increase library conversion efficiency while mitigating index hopping, and single-sided size selection reduces workflow time while increasing library complexity and yields.

Free-up valuable sequencing resources with HyperCap v3

  • Achieve greater success with low-input and poor-quality samples with KAPA HyperPrep and KAPA HyperPlus Library Preparation Kits
  • Explore options to further reduce turnaround time with shorter hybridization steps using our KAPA HyperExome panel (Figure 6)
  • Automate the entire KAPA HyperCap Workflow v3 without the need for a SpeedVac™—now with all hybridization and bead wash steps at 55ºC
  • Reduce workflow complexity and hands-on time with KAPA Universal Enhancing Oligos, eliminating the need for adapter-matched blocking oligos
  • Multiplex up to 16 samples in the same capture, and potentially post-capture multiplex more samples in the same sequencing lane, with KAPA Unique Dual-Indexed Adapters (UDI) Primer Mixes; 97-384 Primer Mixes will be available soon

KAPA HyperExome yields high-quality results with hybridization times as short as 1 hour

(A) Sequencing coverage (B) Capture efficiency, presented as % reads on-target (the percent of mapped, non-duplicate reads overlapping the target region by at least 1 base). (C) Coverage uniformity, presented as Fold-80 base penalty. (D) PCR duplicates, a measure of library complexity (fewer % PCR duplicates=greater library complexity).

METHOD: Target-enriched libraries were generated using the HyperCap Workflow v3.0, with the KAPA HyperPrep Kit and KAPA HyperExome Probes. Single-plex hybridization reactions were carried out at 55°C with 1 μg library and KAPA HyperExome probes, for the following durations: 15 minutes, 1 hour, 4 hours, and 16 hours (standard hybridization is 16 – 20 hours). Normalized, pooled libraries were sequenced on an Illumina NextSeq 500 instrument using the NextSeq High Output kit (2 x 75 bp). Data was down-sampled to 50X raw coverage. For all charts, bars represent the mean from triplicate libraries and error bars indicate the standard deviation. Note: this protocol is still in development and has not yet been fully validated.

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