Figure 1. KAPA SYBR FAST exhibits high-efficiency amplification and library quantification. Amplification efficiencies achieved with KAPA SYBR FAST for ten diverse amplicons, plotted against GC content. All ten amplicons were amplified with similar efficiency (within the optimal range of 95% –105%). This confirms that KAPA SYBR FAST is the ideal enzyme for the amplification of heterogenous populations of DNA fragments, such as NGS libraries.