KAPA HyperCap Workflow v3 delivers complex libraries by combining the high conversion rate of KAPA HyperPrep or KAPA HyperPlus Kits with KAPA Target Enrichment, creating a streamlined, single-vendor supported workflow.
KAPA HyperCap Heredity Panel
For Research Use Only. Not for use in diagnostic procedures.
Enrich for 3332 genes strongly associated with hereditary genetic diseases and oncology
- Enrich for more than 85% of ClinVar pathogenic and likely pathogenic content at >30X by targeting 3332 carefully selected genes with a sequencing-efficient 10 Mb capture panel
- Achieve a high percentage of bases covered at ≥30X for disease-associated genes from key genomic databases
Figure 1. Percent of bases in key genomic databases covered at ≥30X using the KAPA HyperCap Heredity Panel.
Target-enriched libraries were created following the standard KAPA HyperCap Workflow v3 with the KAPA HyperPrep Kit, using 100 ng of gDNA as input (96 library replicates; NA12878 DNA) and 12-plex pre-capture multiplexing for hybrid capture. Final libraries were sequenced on a NovaSeq™ 6000 system (2 x 100 bp) and downsampled to 10 M high-quality filtered clusters prior to analysis.
Project: KAPA HyperCap Fixed Panels, Pleasanton, CA 2021
Maximize sequencing efficiency with >98% target coverage at 30X with 10 M high-quality clusters
- Enrich for more than 85% of ClinVar pathogenic and likely pathogenic content at >30X by targeting 3332 carefully selected genes with a sequencing-efficient 10 Mb capture panel
- Achieve a high percentage of bases covered at ≥30X for disease-associated genes from key genomic databases
Figure 2. Key sequencing metrics generated with the KAPA HyperCap Heredity Panel.
Target-enriched libraries were created following the standard KAPA HyperCap Workflow v3 with the KAPA HyperPlus Kit, using 100 ng of gDNA as input (duplicate samples using gDNA from 16 Coriell cell lines) and 8-plex pre-capture multiplexing. Final libraries were sequenced on a NovaSeq™ 6000 system (2 x 100 bp) and downsampled to 10 M high-quality filtered clusters prior to analysis.
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