Target Enrichment

Enriching target regions of interest for next-generation sequencing

Next-generation sequencing (NGS), with its ability to process hundreds of millions of DNA templates in parallel, has increased sequencing throughput and diminished sequencing costs tremendously1. However, whole genome sequencing is still expensive and does not provide the precision and depth of information required to decipher the roles of individual genes in complex diseases or afford the ability to investigate rare and low-frequency genetic variants. A more cost-effective way is to focus on specific regions of interest using target enrichment strategies. Target enrichment allows focusing of sequencing resources, which leads to reduced cost and simplified analysis2.

Several strategies are employed for enriching for specific genomic regions of interest before sequencing. The methods used predominantly are amplicon-based enrichment using molecular inversion probes (MIP) and hybrid capture enrichment, using probes that hybridize specifically with targeted regions of interest in solution or on a solid surface.

Roche Sequencing offers target enrichment workflows and high-quality kits for either of these methods. These workflows enable focusing sequencing resources for targeted resequencing applications in human genetic disease and cancer research.

Hybridization-based Target Enrichment

Hybridization-based Target Enrichment

Explore the KAPA Target Enrichment portfolio, which use a solution-based capture method for target enrichment. Learn about the HyperCap Workflow v3.0 that combines all reagents and accessories required for library preparation and target enrichment in a single workflow. Utilize KAPA HyperChoice for custom human designs, HyperExplore for custom nonhuman designs, or KAPA HyperExome probes for analyzing the whole exome or for specific disease-associated regions.

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Design Custom NGS Target Enrichment Panels with Ease

Design Custom NGS Target Enrichment Panels with Ease

HyperDesign is a new online tool that uses Roche’s renowned algorithm for probe design and selection, enabling you to customize your target enrichment panels to meet the unique needs of your research. In addition, Roche’s Support Scientists are ready to help you with design planning and optimization.

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NEW KAPA SARS-CoV-2 Target Enrichment Panel

NEW KAPA SARS-CoV-2 Target Enrichment Panel

Monitor the sequence of the SARS-CoV-2 virus and the emergence of new variants with KAPA SARS-CoV-2 Target Enrichment Panel. This panel targets 100% of the reference SARS-CoV-2 genome (NC_045512) and >99.7% of another 183 publicly available SARS-CoV-2 genomes (GenBank).

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Support for SeqCap Target Enrichment

Our SeqCap Target Enrichment portfolio has been discontinued.  If you are an existing SeqCap user and need support, please contact our Scientific Support team for all your inquiries.

US Customers only:

Email: Support.SeqLS@roche.com Phone: 800.262.4911

(Monday - Friday, 9 am - 5 pm EST)

References

1. Can Dijk et al. Trends in Genetics, 2014. Ten years of next generation sequencing technology.

2. Kozarewa et al. Curr Protoc Mol Biol., 2015. Overview of target enrichment strategies.