Enriching target regions of interest for next-generation sequencing
Next-generation sequencing (NGS), with its ability to process hundreds of millions of DNA templates in parallel, has increased sequencing throughput and diminished sequencing costs tremendously1. However, whole genome sequencing is still expensive and does not provide the precision and depth of information required to decipher the roles of individual genes in complex diseases or afford the ability to investigate rare and low-frequency genetic variants. A more cost-effective way is to focus on specific regions of interest using target enrichment strategies. Target enrichment allows focusing of sequencing resources, which leads to reduced cost and simplified analysis2.
Several strategies are employed for enriching for specific genomic regions of interest before sequencing. The methods used predominantly are amplicon-based enrichment using molecular inversion probes (MIP) and hybrid capture enrichment, using probes that hybridize specifically with targeted regions of interest in solution or on a solid surface.
Roche Sequencing offers target enrichment workflows and high-quality kits for either of these methods. These workflows enable focusing sequencing resources for targeted resequencing applications in human genetic disease and cancer research.
Hybridization-based Target Enrichment
Explore the KAPA Target Enrichment portfolio, which use a solution-based capture method for target enrichment. Learn about the HyperCap Workflow v3.0 that combines all reagents and accessories required for library preparation and target enrichment in a single workflow. Utilize KAPA HyperChoice for custom human designs, HyperExplore for custom nonhuman designs, or KAPA HyperExome probes for analyzing the whole exome or for specific disease-associated regions.
Design Custom NGS Target Enrichment Panels with Ease
HyperDesign is a new online tool that uses Roche’s renowned algorithm for probe design and selection, enabling you to customize your target enrichment panels to meet the unique needs of your research. In addition, Roche’s Support Scientists are ready to help you with design planning and optimization.
Combine the performance of hybrid-capture target enrichment with the speed and simplicity of amplicon workflows with KAPA HyperPETE (Primer Extension Target Enrichment) . It is specifically designed and optimized for small design sizes and validated to detect all major somatic variants in cfDNA, FFPE, and RNA samples, including SNVs, short indels, CNVs, MSI, and fusion transcripts (novel and known).
Our SeqCap Target Enrichment portfolio has been discontinued. If you are an existing SeqCap user and need support, please contact our Scientific Support team for all your inquiries.