KAPA Stranded RNA-Seq Kits

Overview

The KAPA Stranded RNA-Seq Kits are the first-generation KAPA RNA library preparation kits that combine the use of a “with-bead” protocol with KAPA HiFi DNA Polymerase selected through our directed evolution technology for high efficiency, high fidelity and low-bias amplification to provide quality RNA libraries with flexible input amounts.

For single-day single-tube streamlined RNA library preparation, we recommend the KAPA RNA HyperPrep Kits.

Thinking of automating library prep on your existing liquid handler?

Roche’s NGS Automation Support Team, in collaboration with non-Roche liquid handling vendors, creates menus of automated methods for KAPA library prep reagents.

Let’s talk about the next steps to get you up and running with automating your NGS library prep protocols. Visit support for non-Roche liquid handlers to learn more.

KAPA Stranded RNA-seq Kits Specifications and Comparison

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Product Name	KAPA Stranded RNA-seq Kits	KAPA Stranded mRNA-seq Kits	KAPA Stranded RNA-seq Kits with RiboErase (HMR)
Library Preparation Duration	6-8 hours	8-10 hours	10-12 hours
Sample Input Amount	10-400 ng into library prep	100 ng - 4 µg into mRNA capture	100 ng - 1 µg into rRNA depletion
Sample Types	High-quality total RNA Degraded or FFPE total RNA Poly(A)-selected and/or depleted RNA	High-quality total RNA	High-quality total RNA Degraded or FFPE total RNA
Species	Eukaryotic (Animal, Plant, etc.) Prokaryotic (Bacteria, etc.)	Eukaryotic (Animal, Plant, etc.)	Human, Mouse, and Rat Other species can be supported via KAPA RiboDesigner custom rRNA depletion
Stranded RNA-sequencing	Yes	Yes	Yes
Amplification	KAPA HiFi DNA Polymerase Compatible with low bias amplification	KAPA HiFi DNA Polymerase Compatible with low bias amplification	KAPA HiFi DNA Polymerase Compatible with low bias amplification
Reaction Purification	KAPA Pure Beads are not included	KAPA Pure Beads are not included	KAPA Pure Beads are not included
Multiplexing	Yes. KAPA Adapters are not supplied, but available separately	Yes. KAPA Adapters are not supplied, but available separately	Yes. KAPA Adapters are not supplied, but available separately
Applications	Whole transcriptome sequencing Gene expression with target enrichment Splice junction and gene fusions with target enrichment	Gene expression Annotation Splice junction and gene fusions	Whole transcriptome Long non-coding RNA Annotation Gene expression Splice junction and gene fusions
Automation Friendly	Yes. Demonstrated and verified on many liquid handling systems. Learn more	Yes. Demonstrated and verified on many liquid handling systems. Learn more	Yes. Demonstrated and verified on many liquid handling systems. Learn more
Genialis™ Data Analysis and Visualization Solution	No	No	No
KAPA RiboDesigner Custom rRNA Depletion Support	N/A	N/A	Yes
Compatible Sequencing Platforms	Illumina Sequencers	Illumina Sequencers	Illumina Sequencers
Storage Information	Kits can be stored for up to 8 months at -20°C	Kits can be stored for up to 8 months at -20°C KAPA mRNA Capture Beads can be stored for up to 8 months at 4˚C	Kits can be stored for up to 8 months at

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KAPA Code	Roche Cat. No	Description	Kit Size
KK8400	07962142001	Stranded RNA-Seq Kit	24 libraries
KK8401	07962169001	Stranded RNA-Seq Kit	96 libraries
KK8420	07962193001	KAPA Stranded mRNA-Seq Kit, with KAPA mRNA Capture Beads	24 libraries
KK8421	07962207001	KAPA Stranded mRNA-Seq Kit, with KAPA mRNA Capture Beads	96 libraries
KK8483	07962282001	KAPA Stranded RNA-Seq Kit with RiboErase (HMR)	24 reactions
KK8484	07962304001	KAPA Stranded RNA-Seq Kit with RiboErase (HMR)	96 reactions

KAPA Stranded RNA-seq Kits include reagents for RNA fragmentation, 1st strand cDNA synthesis, 2nd strand synthesis/marking, and cDNA library preparation, including A-tailing, ligation, and library amplification.

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KAPA Code	Roche Cat. No	Description	Kit Size
KK8000	07983271001	KAPA Pure Beads (5 mL)	5 mL
KK8001	07983280001	KAPA Pure Beads (30 mL)	30 mL
KK8002	07983298001	KAPA Pure Beads (60 mL)	60 mL
KK8721	08278539001	KAPA Adapter Dilution Buffer (25 mL)	25 mL
KK8727*	08861919702	KAPA Unique Dual-Indexed Adapter Kit, (15 µM)	96 adapters x 20 µl each

*Contains KAPA Adapter Dilution Buffer, as well as three additional sealing films to support multiple use