Overview

The KAPA Stranded RNA-Seq Kits are the first-generation KAPA RNA library preparation kits that combine the use of a “with-bead” protocol with KAPA HiFi DNA Polymerase selected through our directed evolution technology for high efficiency, high fidelity and low-bias amplification to provide quality RNA libraries with flexible input amounts.

For single-day single-tube streamlined RNA library preparation, we recommend the KAPA RNA HyperPrep Kits.

KAPA Stranded RNA-seq Kits Specifications and Comparison

Product Name KAPA Stranded RNA-seq Kits KAPA Stranded mRNA-seq Kits KAPA Stranded RNA-seq Kits with RiboErase (HMR)
Library Preparation Duration 6-8 hours 8-10 hours 10-12 hours
Sample Input Amount 10-400 ng into library prep 100 ng - 4 µg into mRNA capture 100 ng - 1 µg into rRNA depletion
Sample Types

High-quality total RNA

Degraded or FFPE total RNA

Poly(A)-selected and/or depleted RNA

High-quality total RNA

High-quality total RNA

Degraded or FFPE total RNA

Species

Eukaryotic (Animal, Plant, etc.)

Prokaryotic (Bacteria, etc.)

Eukaryotic (Animal, Plant, etc.)

Human, Mouse, and Rat

Other species can be supported via KAPA RiboDesigner custom rRNA depletion

Stranded RNA-sequencing Yes Yes Yes
Amplification

KAPA HiFi DNA Polymerase

Compatible with low bias amplification

KAPA HiFi DNA Polymerase

Compatible with low bias amplification

KAPA HiFi DNA Polymerase

Compatible with low bias amplification

Reaction Purification KAPA Pure Beads are not included KAPA Pure Beads are not included KAPA Pure Beads are not included
Multiplexing Yes.  KAPA Adapters are not supplied, but available separately Yes.  KAPA Adapters are not supplied, but available separately Yes.  KAPA Adapters are not supplied, but available separately
Applications

Whole transcriptome sequencing

Gene expression with target enrichment

Splice junction and gene fusions with target enrichment

Gene expression

Annotation

Splice junction and gene fusions

Whole transcriptome

Long non-coding RNA Annotation

Gene expression

Splice junction and gene fusions

Automation Friendly Yes.  Demonstrated and verified on many liquid handling systems.
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Yes.  Demonstrated and verified on many liquid handling systems.
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Yes.  Demonstrated and verified on many liquid handling systems.
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Genialis™ Data Analysis and Visualization Solution No No No
KAPA RiboDesigner Custom rRNA Depletion Support N/A N/A Yes   Learn more
Compatible Sequencing Platforms Illumina Sequencers Illumina Sequencers Illumina Sequencers
Storage Information

Kits can be stored for up to 8 months at -20°C

Kits can be stored for up to 8 months at -20°C

KAPA mRNA Capture Beads can be stored for up to 8 months at 4˚C

Kits can be stored for up to 8 months at -20°C

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