Product Name | KAPA Stranded RNA-seq Kits | KAPA Stranded mRNA-seq Kits | KAPA Stranded RNA-seq Kits with RiboErase (HMR) |
Library Preparation Duration | 6-8 hours | 8-10 hours | 10-12 hours |
Sample Input Amount | 10-400 ng into library prep | 100 ng - 4 µg into mRNA capture | 100 ng - 1 µg into rRNA depletion |
Sample Types | High-quality total RNA Degraded or FFPE total RNA Poly(A)-selected and/or depleted RNA |
High-quality total RNA | High-quality total RNA Degraded or FFPE total RNA |
Species | Eukaryotic (Animal, Plant, etc.) Prokaryotic (Bacteria, etc.) |
Eukaryotic (Animal, Plant, etc.) | Human, Mouse, and Rat Other species can be supported via KAPA RiboDesigner custom rRNA depletion |
Stranded RNA-sequencing | Yes | Yes | Yes |
Amplification | KAPA HiFi DNA Polymerase Compatible with low bias amplification |
KAPA HiFi DNA Polymerase Compatible with low bias amplification |
KAPA HiFi DNA Polymerase Compatible with low bias amplification |
Reaction Purification | KAPA Pure Beads are not included | KAPA Pure Beads are not included | KAPA Pure Beads are not included |
Multiplexing | Yes. KAPA Adapters are not supplied, but available separately | Yes. KAPA Adapters are not supplied, but available separately | Yes. KAPA Adapters are not supplied, but available separately |
Applications | Whole transcriptome sequencing Gene expression with target enrichment Splice junction and gene fusions with target enrichment |
Gene expression Annotation Splice junction and gene fusions |
Whole transcriptome Long non-coding RNA Annotation Gene expression Splice junction and gene fusions |
Automation Friendly | Yes. Demonstrated and verified on many liquid handling systems. Learn more |
Yes. Demonstrated and verified on many liquid handling systems. Learn more |
Yes. Demonstrated and verified on many liquid handling systems. Learn more |
Genialis™ Data Analysis and Visualization Solution | No | No | No |
KAPA RiboDesigner Custom rRNA Depletion Support | N/A | N/A | Yes Learn more |
Compatible Sequencing Platforms | Illumina Sequencers | Illumina Sequencers | Illumina Sequencers |
Storage Information | Kits can be stored for up to 8 months at -20°C |
Kits can be stored for up to 8 months at -20°C KAPA mRNA Capture Beads can be stored for up to 8 months at 4˚C |
Kits can be stored for up to 8 months at -20°C |