KAPA HyperPrep Kits

For Research Use Only. Not for use in diagnostic procedures.

KAPA HyperPrep product
Overview

KAPA HyperPrep Kits offer a streamlined library preparation protocol that combines several enzymatic steps and eliminates bead cleanups to significantly reduce library preparation time and improve consistency. The novel, single-tube chemistry offers further improvements to library construction efficiency, particularly for challenging samples such as FFPE and cell-free DNA. KAPA HyperPrep Kits offer complete library preparation solution with KAPA Adapters and KAPA Pure Beads.

Features and Benefits of KAPA HyperPrep Kits
  • Robust and reproducible library construction in 2-3 hours with high conversion rates and library complexity, particularly for FFPE
  • PCR-free workflows from lower sample inputs and improved sequence coverage for workflows with PCR
  • Compatibility with a wide range of sample types and inputs, and flexibility with respect to fragment size, adapter design and library amplification
  • Qualified automation methods
  • Validated with the HyperCap Workflow v3.0

Product highlights

Improved library yields and quality
  • Higher library yields translating to greater molecular complexity
  • Higher conversion of input DNA to adapter-ligated library
  • Fewer cycles of amplification with KAPA HiFi DNA Polymerase, resulting in lower duplication rates and improved coverage

View comparison data for higher conversion of input DNA to adapter-ligated library by KAPA HyperPrep Kit compared to Illumina TruSeq Nano DNA Kits or  NEBNext® Ultra™ II FS Kit.

Minimal amplification bias
  • Kits with or without an amplification module
  • Lower PCR bias resulting in more uniform coverage in PCR workflows

See performance data showing high coverage uniformity using KAPA HyperPrep Kits and minimal amplification bias with KAPA HiFi polymerase.

High coverage of FFPE samples
  • Higher library yield, higher library complexity and fewer PCR cycles
  • Lower duplication rates and higher coverage

View data showing higher library diversity, lower duplication rates and improved coverage and using KAPA HyperPrep Kit in comparison with TruSeq Nano DNA Kit.

 

Improved performance with cell-free DNA
  • Ability to optimize adapter:insert ratios for challenging sample types, such as cell-free DNA
  • Higher library density compared to competitor kit*
  • Comparable duplication rates to vendor kits specific for low-input applications

See comparative data for improved library diversity using KAPA HyperPrep Kit compared to competitor kit. View data showing comparable duplication rates by KAPA HyperPrep Kit as those by a competitor kit designed specifically for low-input applications.

Available Products

Are you sequencing low-input, FFPE or high quality DNA?  RNA?  Check out these Kapa NGS products to improve your workflow and results:

Specifications

  • Compatible Platform

    All Illumina sequencing platforms and the Element AVITI

  • Library Type

    DNA

  • Starting Material

    Fragmented DNA, cDNA, PCR amplicons, cfDNA, FFPE

  • Input Amount

    1 ng – 1 µg

Kits can be stored for up to 12 months at -20˚C.

Kits contain KAPA End Repair & A-Tailing Buffer, KAPA End Repair & A-Tailing Enzyme, KAPA Ligation Buffer, KAPA DNA Ligase, KAPA HiFi HotStart ReadyMix and KAPA Library Amplification Primer Mix

 

Components

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Components

  Kit Code End-Repair
&A-Tailing
buffer
End-Repair
& A-Tailing
Enzyme
Ligation
Buffer
Ligase KAPA HiFi
HotStart
ReadyMix
KAPA
Library
Amplification
Primer Mix
KAPA HyperPrep Kit 07962312001 (8 rxn) X X X X X X
07962347001 (24 rxn)            
07962363001 (96 rxn)            
KAPA HyperPrep Kit, PCR-free 07962339001 (8 rxn) X X X X    
07962355001 (24 rxn)            
07962371001 (96 rxn)            
KAPA Code
Roche Cat. No
Description
Kit Size
How to buy
KK8503
07962355001
Without amplification module 24 reactions
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KK8505
07962371001
Without amplification module 96 reactions
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KK8502
07962347001
With KAPA Library Amplification Primer Mix (10X) 24 reactions
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KK8504
07962363001
With KAPA Library Amplification Primer Mix (10X) 96 reactions
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Accessory Products

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Accessory Products

KAPA Single-Indexed Adapter Set A contains indices 2, 4, 5, 6, 7, 12, 13, 14, 15, 16, 18 and 19, whereas Set B contains indices 1, 3, 8, 9, 10, 11, 20, 21, 22, 23, 25, 27. All KAPA Single- and Dual-Indexed Adapter Kits contain KAPA Adapter Dilution Buffer. KAPA Dual-Indexed Adapter Kits also contain three additional sealing films to support multiple use.

KAPA Single-Indexed Adapter Set A contains indices 2, 4, 5, 6, 7, 12, 13, 14, 15, 16, 18 and 19, whereas Set B contains indices 1, 3, 8, 9, 10, 11, 20, 21, 22, 23, 25, 27. All KAPA Single- and Dual-Indexed Adapter Kits contain KAPA Adapter Dilution Buffer. KAPA Dual-Indexed Adapter Kits also contain three additional sealing films to support multiple use.

KAPA Code
Roche Cat. No
Description
Kit Size
How to buy
KK8000
07983271001
KAPA Pure Beads (5 mL) 5 mL
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KK8001
07983280001
KAPA Pure Beads (30 mL) 30 mL
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KK8002
07983298001
KAPA Pure Beads (60 mL) 60 mL
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KK8721
08278539001
KAPA Adapter Dilution Buffer (25 mL) 25 mL
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KK8727
08861919702

KAPA Unique Dual-Indexed Adapter Kit, (15 µM)

96 adapters x 20 µl each
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Additional pack sizes are available for KAPA HyperPure Beads. Please speak to your local sales representative.