1. Roche Sequencing | United States
  2. Library Preparation
  3. DNA Library Preparation
  4. KAPA Hyperprep

KAPA HyperPrep Kits

Overview

KAPA HyperPrep Kits offer a streamlined library preparation protocol that combines several enzymatic steps and eliminates bead cleanups to significantly reduce library preparation time and improve consistency. The novel, single-tube chemistry offers further improvements to library construction efficiency, particularly for challenging samples such as FFPE and cell-free DNA. KAPA HyperPrep Kits offer complete library preparation solution with KAPA Adapters and KAPA Pure Beads.

Features and Benefits of KAPA HyperPrep Kits

  • Robust and reproducible library construction in 2-3 hours with high conversion rates and library complexity, particularly for FFPE
  • PCR-free workflows from lower sample inputs and improved sequence coverage for workflows with PCR
  • Compatibility with a wide range of sample types and inputs, and flexibility with respect to fragment size, adapter design and library amplification
  • Qualified automation methods
  • Validated with the HyperCap Workflow v2.0

Product Highlights

Improved library yields and quality

  • Higher library yields translating to greater molecular complexity
  • Higher conversion of input DNA to adapter-ligated library
  • Fewer cycles of amplification with KAPA HiFi DNA Polymerase, resulting in lower duplication rates and improved coverage

View comparison data for higher conversion of input DNA to adapter-ligated library by KAPA HyperPrep Kit compared to Illumina TruSeq Nano DNA Kits or NEBNext Ultra Kit.

Minimal amplification bias

  • Kits with or without an amplification module
  • Lower PCR bias resulting in more uniform coverage in PCR workflows

See performance data showing high coverage uniformity using KAPA HyperPrep Kits and minimal amplification bias with KAPA HiFi polymerase

High coverage of FFPE samples

  • Higher library yield, higher library complexity and fewer PCR cycles
  • Lower duplication rates and higher coverage

View data showing higher library diversity, lower duplication rates and improved coverage and using KAPA HyperPrep Kit in comparison with TruSeq Nano DNA Kit.

Improved performance with cell-free DNA

  • Ability to optimize adapter:insert ratios for challenging sample types, such as cell-free DNA
  • Higher library density compared to competitor kit*
  • Comparable duplication rates to vendor kits specific for low-input applications

See comparative data for improved library diversity using KAPA HyperPrep Kit compared to competitor kit. View data showing comparable duplication rates by KAPA HyperPrep Kit as those by a competitor kit designed specifically for low-input applications.

Available Products

Are you sequencing low-input, FFPE or high quality DNA?  RNA?  Check out these Kapa NGS products to improve your workflow and results:

*Data on file.

For Research Use Only. Not for use in diagnostic procedures.
KAPA and SEQCAP are trademarks of Roche. All other product names and trademarks are the property of their respective owners.