1. Roche Sequencing
  2. Library Preparation
  3. DNA Library Preparation
  4. KAPA HTP LTP

KAPA HTP/LTP Library Preparation Kits

KAPA HTP/LTP Library Preparation Kits contain optimally formulated enzymes selected through our directed evolution technology and implement a highly optimized “with bead” strategy that enable higher library construction efficiency and facilitate automation.  Kits offer higher success rates than conventional library construction kits — particularly from lower inputs and challenging samples such as FFPE and ChIP DNA —  in both high- and low-throughput sample preparation pipelines. KAPA HTP/LTP Library Preparation Kits provide a complete library preparation solution with KAPA Adapters and KAPA Pure Beads.

Features and Benefits of KAPA HTP/LTP Library Preparation Kits

• Automation-friendly, highly optimized “with-bead” protocol facilitating minimal sample loss with no physical transfer of library material between enzymatic reactions

• Improved coverage uniformity as a result of low-bias library amplification with KAPA HiFi enzyme 

• Compatibility with a wide range of DNA sample types, inputs and sequencing applications, and flexibility with respect to fragment size, adapter design, and library amplification 

• Qualified automation methods

 

Product Highlights

Greater molecular complexity

  • Higher adapter-ligated library yields, translating to higher library complexity
  • Fewer cycles of amplification needed, resulting in lower PCR duplication rates, which are critical for library construction from lower input and challenging samples

View comparative data showing higher number of unique library molecules and reduced duplication rates using the KAPA HTP/LTP Library Preparation Kit compared to a competitor kit.

Reduced amplification bias and improved coverage

  • Reduced PCR bias* using KAPA HiFi enzyme
  • Improved coverage uniformity of across the entire range of genomic GC content













Automation with qualified scripts

  • Qualified methods available for major liquid handling platforms used in NGS, such as PerkinElmer Sciclone NGS and NGSx, Beckman Coulter Biomek FXp and FX dual-pod, Agilent Technologies NGS Workstation Option B, and Eppendorf® epMotion 5075 TMX

View data on more uniform coverage distribution across varying G-C content using KAPA HTP/LTP Library Preparation Kit compared to another vendor.

*Data on file.

Research Use Only. Not for use in diagnostic procedures.
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