What is RNA library preparation?
The first step of RNA-sequencing (RNA-seq) is RNA library preparation which consists of the following general steps:
The first step of RNA-sequencing (RNA-seq) is RNA library preparation which consists of the following general steps:
Each single-stranded RNA transcript in a cell is synthesized from one of the two strands of DNA. During RNA library preparation, the RNA transcript is copied back into complementary DNA (cDNA). Strand-specific RNA library preparation improves RNA-seq by accurately identifying antisense transcripts and non-coding RNA and distinguish the boundaries of closely situated or overlapping genes.
Roche’s RNA library preparation kits offer stranded and streamlined workflows for a wide a wide variety of RNA-seq applications, including whole-transcriptome sequencing, tumor profiling using degraded inputs, and sequencing of blood-derived RNA.
As our latest generation of RNA library preparation kits, KAPA RNA HyperPrep Kits utilize a novel chemistry and combines enzymatic steps to achieve fewer reaction purifications and yield high-quality RNA-seq libraries in a single day. They also offer flexible workflow options that include the enrichment of desired transcripts by selective mRNA capture or rRNA depletion.
KAPA Stranded RNA-seq Kits are our first generation RNA library preparation kits that combine a “with-bead” protocol with the robustness of KAPA HiFi DNA Polymerase to yield high-quality RNA-seq libraries.
Roche’s NGS Automation Support Team, in collaboration with non-Roche liquid handling vendors, creates menus of automated methods for KAPA library prep reagents.
Let’s talk about the next steps to get you up and running with automating your NGS library prep protocols. Visit support for non-Roche liquid handlers to learn more.
For Research Use Only. Not for use in diagnostic procedures.