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As a part of the dynamic Roche Integrated NGS workflow portfolio, KAPA HyperPure Beads offer a highly consistent solution for size selection and reaction purification in DNA library construction workflows for next-generation sequencing.
Achieve high recovery of DNA across a wide range of input amounts
Reduce sequencing costs and maximize library diversity with improved bead wash efficiency
Remove undesirable reaction components with fast bead cleanup steps
Choose from five pack sizes for workflow flexibility
Figure 1A. Recovery of unfragmented human gDNA. (n=4)
KAPA HyperPure Beads provide superior recovery performance to Supplier A in DNA workflows.
Highly degraded DNA obtained from FFPE clinical research samples (n=12); moderately degraded DNA obtained from Horizon Reference FFPE DNA (n=9). For both (A) and (B), KAPA HyperPure Beads and Supplier A beads were used at a 3X ratio to clean up DNA inputs of various amounts. Recovery was measured using the Qubit Fluorometer 3 dsDNA HS Assay Kit before and after cleanup. Error bars represent standard deviation.
Figure 1B. Recovery of highly degraded and moderately degraded formalin-fixed paraffin-embedded (FFPE) human genomic DNA.
KAPA HyperPure Beads provide superior recovery performance to Supplier A in DNA workflows.
Highly degraded DNA obtained from FFPE clinical research samples (n=12); moderately degraded DNA obtained from Horizon Reference FFPE DNA (n=9). For both (A) and (B), KAPA HyperPure Beads and Supplier A beads were used at a 3X ratio to clean up DNA inputs of various amounts. Recovery was measured using the Qubit Fluorometer 3 dsDNA HS Assay Kit before and after cleanup. Error bars represent standard deviation.
Figure 2A. Postfragmentation size selection of input DNA. High-quality hgDNA was mechanically fragmented with a Covaris E220 Focused Ultrasonicator using conditions optimized to yield a fragment length of 250 – 400 bp.
Figure 2B. Post-ligation size selection. Libraries were prepared from 100 ng of fragmented input DNA using the KAPA HyperPrep Kit and KAPA Unique Dual-Index Adapters.
Figure 2C. Post-amplification size selection. Libraries were PCRamplified and final amplified libraries were subjected to size selection.
Streamline preparation of high-quality NGS libraries with KAPA DNA Library Prep Kits-- flexible, automation-friendly and compatible with many sample types.
KAPA Library Amplification kits include KAPA HiFi DNA Polymerase, a novel enzyme engineered using our directed evolution technology for ultra-high fidelity and robustness.
High-quality adapters for multiplexed sequencing on 2- and 4-channel Illumina® instruments, with patterned or non-patterned flow cells. Compatible with all KAPA Hyper library preparation kits, the HyperCap Workflow with Roche Universal Blocking Oligos, and PCR-free workflows. MC-US-10699
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KAPA HyperPure Beads may be shipped on dry ice which does not affect product performance. Upon receipt they must be stored at 2°C to 8°C.
