DNA Library Preparation

Preparation of a DNA library for next generation sequencing (NGS) platforms entails several steps that have a direct influence on the quality of the sequencing library, and ultimately on the reliability of sequencing results.  Roche Sequencing Solutions offers DNA library preparation kits containing high-quality enzymes and reaction buffers developed through our Directed Evolution Technology. The KAPA Library Preparation Kits include modules required for end repair, A-tailing, ligation, and amplification (for Illumina platforms) and offer complete library preparation solutions with KAPA Adapters and KAPA Pure Beads.

• Compatibility with a wide variety of DNA inputs and sample types and high yields of adapter-ligated library molecules 

• Reduced PCR amplification bias resulting in improved sequencing coverage compared to other options*

• Kits without an amplification module for PCR-free workflows

• Ultra-pure, high-quality library construction reagents supplied in convenient master-mix formats

• Competitive per library cost

Roche Sequencing Solutions offers three generations of KAPA Library Preparation Kits: HTP/LTP, HyperPrep and HyperPlus, for library construction on the Illumina platform.