For Research Use Only. Not for use in diagnostics procedures.
Roche Presenters: Adeline Pek, MSc, MPharm | Liu Xi, MSc | Jo-Anne Penkler, MSc
We would like to invite you to learn about a new suite of NGS technologies from Roche Sequencing that are designed to work together to target specific genomic regions, identify variants, and provide biological insights into the results.
In this webinar, we will highlight 3 of these tools:
*Product in development. Not for sale in the United States.
In the US, NAVIFY Mutation Profiler, and NAVIFY Mutation Caller are for Research Use Only. Not for use in diagnostics procedures.
Presenter: Dustin Masser, PhD, Roche Sequencing & Life Science
KAPA HyperPETE is a novel target enrichment technology that is designed to combine the performance of hybrid-capture with the ease-of-use and turnaround time of amplicon-based workflows. In addition, it is optimized to detect all major somatic variant types (SNV, CNV, InDel, MSI status, and novel fusion transcript partners), and can be performed on tissue DNA, plasma DNA, and tissue RNA samples.
In this webinar, we will review:
Presenter: Mariana Fitarelli Kiehl, PhD, Sr. Applications Scientist, Roche Sequencing & Life Science
High-throughput NGS library preparation and expanded sequencing capacity demand greater scalability in nucleic acid extraction workflows, with less hands-on time.
In this webinar, we will:
Presenter: Mariana Fitarelli Kiehl, PhD, Sr. Applications Scientist, Roche Sequencing & Life Science
In many scRNA-seq workflows, the initial steps employ the template switching method for cDNA synthesis followed by cDNA normalization prior to library preparation. Roche’s KAPA HyperPrep Kit is effective across a 30-fold range of cDNA input amounts, eliminating the need for cDNA normalization in scRNA-seq workflows while also generating complex, uniform libraries and high-quality sequencing data.
In this webinar, we:
Presenter: Jason Liu, PhD, Field Application Scientist, Roche Sequencing & Life Science
Accurate DNA amplification is essential for NGS-based workflows, including CRISPR gene editing, screening of CRISPR clones for on-target and off-target genome modifications, and single-cell RNA sequencing (scRNA). The robust, high-fidelity KAPA HiFi polymerase enables fast, efficient workflows for each of these methods.
In this webinar, we demonstrate how KAPA HiFi DNA polymerase and KAPA NGS Library Preparation Kits enable robust workflows for:
Presenter: Sarah Trusiak, PhD, Sr. Application Scientist, Roche Sequencing & Life Science
New SARS-CoV-2 strains are continuing to emerge, potentially impacting the effectiveness of existing vaccines. Thus, the ability to determine the genomic sequences in samples is critical for effective surveillance and tracking of the virus. Because viral RNA represents only a small fraction of the nucleic acids in a given sample, the development of robust, sensitive sequencing methods is essential.
We have developed a method for target-enriched RNA-seq with hybridization-based target enrichment (TE) of the SARS-CoV-2 genome, enabling high-throughput analysis to discover new viral mutations and track strain transmission. The workflow utilizes the KAPA SARS-CoV-2 TE panel and the HyperCap v3 workflow.
In this webinar, we:
Presenter: Jason Liu, PhD, Field Application Scientist, Roche Sequencing & Life Science
In this webinar, we provided an overview of how NGS technologies are advancing our understanding of the SARS-CoV-2 virus and the COVID-19 pandemic. We will review:
Presenter: Mariana Fitarelli Kiehl, PhD, Sr. Applications Scientist, Roche Sequencing & Life Science
Providing targeted next-generation sequencing (NGS) results faster is a common challenge facing labs today. By reducing the time required for hybridization from 16 hours to less than an hour—without compromising performance—you can dramatically increase your lab’s productivity.
In this webinar, we:
Presenter: Eleanor Cowley, M.S., Sr. NGS Field Automation Scientist, Roche Sequencing & Life Science
Automating RNA-sequencing (RNA-seq) library preparation offers advantages such as higher sample throughput, less hands-on time, fewer human errors, greater reproducibility, and better process control and workflow standardization. However, developing and implementing an automated RNA-seq library prep protocol on a liquid handling platform can be challenging and time-consuming.
In this webinar, we discussed the automation of RNA-seq library prep, including:
Presenter: Drew Cheney, Field Application Specialist, Roche Sequencing and Life Science
RNA-sequencing (RNA-seq) and the study of transcriptomes have become essential in both molecular and clinical research. Many researchers and sequencing service providers have found RNA-seq difficult to adopt due to various challenges.
In this webinar, we discussed:
Presenter: Jonathan Nowacki, Senior Technical Services Consultant - Bioinformatics, Roche Sequencing and Life Science
The right set of NGS tools can save your business— or your lab— valuable sequencing resources. To maximize efficiency and effectiveness, ask yourself: (1) Which approach is best for answering specific experimental questions? (2) Is this the most cost-effective method to get the job done?
In this webinar, we discussed:
Presenter:
Teri Rambo Mueller, Field Applications Consultant, Roche Sequencing and Life Science;
Daniel Kim, Instrument Sales Specialist, Roche Sequencing and Life Science
In the age of precision medicine, molecular testing by next-generation sequencing and PCR are transforming oncology decision making. The clinical value of that molecular testing is significantly enhanced by precise dissection of formalin-fixed paraffin-embedded tissue samples.
The AVENIO Millisect System is an automated, high-performance FFPE tissue-dissection system that enables the precise and consistent recovery of tissue areas of interest for molecular pathology. Easily integrated into a variety of clinical workflow configurations, the AVENIO Millisect System can help labs efficiently extract more clinically relevant information from every sample. Accurate dissection improves the results of diagnostic testing by reducing false negatives and allowing identification of additional potential therapeutic targets.
In this webinar, we:
Presenter: Drew Cheney, Field Application Specialist, Roche Sequencing and Life Science
Building on over 10 years of design expertise, Roche is pleased to introduce the KAPA Target Enrichment Portfolio. These new probes for hybridization-based target enrichment are available in the new KAPA HyperExome or in Custom Panels that you develop using HyperDesign , our new online development tool.
In this webinar, we:
Presenter: Dr. Sarah Trusiak, PhD, Senior Applications Scientist Roche Sequencing and Life Science
Since the emergence of the SARS-CoV-2 virus in late 2019, many research groups have pivoted their focus to studying the virus and its associated disease, COVID-19. RNA-seq offers a powerful, high-throughput tool for studying both the genome of RNA viruses, such as SARS-CoV-2, and the resulting transcriptional changes in a host during viral infection.
In this webinar, we:
We know there’s a lot to learn about NGS, from sample prep to workflow optimization to understanding sequencing metrics and putting it all together while making the most of your sequencing budget-— including time, reagents, and bioinformatic analysis—isn’t easy.
That’s why we created this collection of short videos — to help answer your NGS questions and offer guidance for getting the most out of every sample, including DNA, RNA, and target-enriched NGS libraries.
Get the next Ask a Scientist video in your inbox.
For Research Use Only. Not for use in diagnostics procedures.