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KAPA HyperPETE: The Performance of hybrid-capture TE with the speed & simplicity of amplicon workflows
Thursday, October 14th, 2021, 12:00 p.m. ET, 9 a.m. PT
Presenter: Dustin Masser, PhD, Roche Sequencing & Life Science
KAPA HyperPETE is a novel target enrichment technology that is designed to combine the performance of hybrid-capture with the ease-of-use and turnaround time of amplicon-based workflows. In addition, it is optimized to detect all major somatic variant types (SNV, CNV, InDel, MSI status, and novel fusion transcript partners), and can be performed on tissue DNA, plasma DNA, and tissue RNA samples.
In this webinar, we will review:
- The science behind Primer Extension Target Enrichment (PETE) technology
- How KAPA HyperPETE reduces bias and increases the accuracy of sequencing data
- Performance metrics for KAPA HyperPETE panels for somatic tissue DNA, somatic tissue RNA, and cfDNA inputs
Target Enriched RNA-seq for SARS-CoV-2 Surveillance and Genome Analysis
Wednesday, March 24th 2021, 1:00 p.m. ET, 10 a.m. PT
Presenter: Sarah Trusiak, PhD, Sr. Application Scientist, Roche Sequencing & Life Science
New SARS-CoV-2 strains are continuing to emerge, potentially impacting the effectiveness of existing vaccines. Thus, the ability to determine the genomic sequences in samples is critical for effective surveillance and tracking of the virus. Because viral RNA represents only a small fraction of the nucleic acids in a given sample, the development of robust, sensitive sequencing methods is essential.
We have developed a method for target-enriched RNA-seq with hybridization-based target enrichment (TE) of the SARS-CoV-2 genome, enabling high-throughput analysis to discover new viral mutations and track strain transmission. The workflow utilizes the KAPA SARS-CoV-2 TE panel and the HyperCap v3 workflow.
In this webinar, we:
- Review the HyperCap v3 workflow using the KAPA SARS-CoV-2 TE panel
- Describe an accelerated workflow with a shortened 1 hour hybridization
- Discuss optional workflow modifications to increase SARS-CoV-2 reads
- Present performance data for samples containing varying amounts of SARS-CoV-2 RNA in a background of human RNA
- Show that the KAPA SARS-CoV-2 TE panel can detect mutations from multiple strains within a single reaction
Single-day Hybridization-based Capture with KAPA Target Enrichment Probes for NGS
Wednesday, January 27th 2021, 1:00 p.m. ET, 10 a.m. PT
Presenter: Mariana Fitarelli Kiehl, PhD, Sr. Applications Scientist, Roche Sequencing & Life Science
Providing targeted next-generation sequencing (NGS) results faster is a common challenge facing labs today. By reducing the time required for hybridization from 16 hours to less than an hour—without compromising performance—you can dramatically increase your lab’s productivity.
In this webinar, we:
- Review the basic steps of the KAPA HyperCap Workflow v3
- Present sequencing data for libraries generated with enzymatically and mechanically-fragmented DNA and a range of hybridization times during target enrichment
- Discuss the effectiveness of the KAPA HyperCap Workflow v3 with hybridization times as short as 15 minutes using the KAPA HyperExome panel
Automating RNA-seq Library Preparation
Wednesday, December 16th 2020, 1:00 p.m. EST, 10 a.m. PST
Presenter: Eleanor Cowley, M.S., Sr. NGS Field Automation Scientist, Roche Sequencing & Life Science
Automating RNA-sequencing (RNA-seq) library preparation offers advantages such as higher sample throughput, less hands-on time, fewer human errors, greater reproducibility, and better process control and workflow standardization. However, developing and implementing an automated RNA-seq library prep protocol on a liquid handling platform can be challenging and time-consuming.
In this webinar, we discussed the automation of RNA-seq library prep, including:
- Benefits of automating RNA-seq library prep
- Tips and tricks of NGS automation
- Overview of the Roche Sequencing automation program
- Examples of automated workflows for KAPA RNA HyperPrep
Overcome the Challenges of RNA-seq Using KAPA RNA HyperPrep
Tuesday, November 3th 2020, 1:00 p.m. ET, 10 a.m. PT
Presenter: Drew Cheney, Field Application Specialist, Roche Sequencing and Life Science
RNA-sequencing (RNA-seq) and the study of transcriptomes have become essential in both molecular and clinical research. Many researchers and sequencing service providers have found RNA-seq difficult to adopt due to various challenges.
In this webinar, we discussed:
- What RNA-seq is and why it is crucial in research
- Unique RNA-seq challenges in sample handling, library preparation, and data analysis
- KAPA RNA HyperPrep workflows and how they can help overcome these challenges
- Q&A with an experienced Roche field application specialist
Investing in the Right Sample Prep Solutions to Maximize your Sequencing Resources
Wednesday, October 14th 2020, 1:00 p.m. ET, 10 a.m. PT
Presenter: Jonathan Nowacki, Senior Technical Services Consultant - Bioinformatics, Roche Sequencing and Life Science
The right set of NGS tools can save your business— or your lab— valuable sequencing resources. To maximize efficiency and effectiveness, ask yourself: (1) Which approach is best for answering specific experimental questions? (2) Is this the most cost-effective method to get the job done?
In this webinar, we discussed:
- The economics of sequencing; Where are your resources going?
- GC bias; How does GC bias impact the value?
- Analysis of dropouts (zero coverage) and low-coverage genes; How do these impact value?
- A customizable metric for analyzing value, enabling you to gain insights to improve efficiency
New KAPA Target Enrichment: Better by Design
Wednesday, September 2nd 2020, 1:00 p.m. ET, 10 a.m. PT
Presenter: Drew Cheney, Field Application Specialist, Roche Sequencing and Life Science
Building on over 10 years of design expertise, Roche is pleased to introduce the KAPA Target Enrichment Portfolio. These new probes for hybridization-based target enrichment are available in the new KAPA HyperExome or in Custom Panels that you develop using HyperDesign , our new online development tool.
In this webinar, we:
- Shared the improved benefits offered by KAPA Target Enrichment probes, especially when paired with our KAPA library prep workflows
- Compared the performance and efficiency of KAPA Target Enrichment probes and the HyperCap Workflow v3 to other widely used targeted enrichment products
- Introduced HyperDesign, our new probe design interface that provides customized probe selection, and live expert assistance at your fingertips
RNA-Seq Applications for SARS-CoV-2 Research
Wednesday, August 26th 2020, 1:00 p.m. ET, 10 a.m. PT
Presenter: Dr. Sarah Trusiak, PhD, Senior Applications Scientist Roche Sequencing and Life Science
Since the emergence of the SARS-CoV-2 virus in late 2019, many research groups have pivoted their focus to studying the virus and its associated disease, COVID-19. RNA-seq offers a powerful, high-throughput tool for studying both the genome of RNA viruses, such as SARS-CoV-2, and the resulting transcriptional changes in a host during viral infection.
In this webinar, we:
- Presented several applications of RNA-seq in viral research, including host response, drug discovery, and viral genome mutation analysis, while highlighting examples from recent preprints and publications
- Discussed specific RNA-seq workflows for each viral application
Welcome to the Ask a Scientist video collection
We know there’s a lot to learn about NGS, from sample prep to workflow optimization to understanding sequencing metrics and putting it all together while making the most of your sequencing budget-— including time, reagents, and bioinformatic analysis—isn’t easy.
That’s why we created this collection of short videos — to help answer your NGS questions and offer guidance for getting the most out of every sample, including DNA, RNA, and target-enriched NGS libraries.
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