Figure 2: KAPA EvoPrep chemistry enables high library conversion across a range of input DNA
0.1 ng - 500 ng of Covaris-sheared human genomic DNA was used to prepare libraries with KAPA Universal Adapters (with KAPA UDI Primer Mixes) at the recommended adapter: insert molar ratio following the KAPA EvoPrep and KAPA HyperPrep Kit Instructions for Use. Libraries were amplified for various cycles dependent on DNA input to enable visualization. Electropherograms were generated with LabChip GX Touch NGS 3K Assay.
*Non-validated input (outside of the input range) for KAPA HyperPrep Kit - optimized cycle number for KAPA EvoPrep Kit inputs used