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For Research Use Only. Not for use in diagnostic procedures.
Overview
KAPA HyperPure Beads offer a tunable and highly consistent solution for reaction purification and size selection in DNA next-generation sequencing (NGS) library construction workflows.
Features and Benefits
High recovery of single- and double-stranded DNA (1 ng – 5 μg) in a single cleanup
Fast and efficient cleanups to remove unwanted reaction components
Easy substitution into bead-based workflows
Adjustable size selection
Automation friendly
Larger pack sizes for higher throughput
Resources
Technical documents
Product highlights
Seamless Integration into NGS Workflows
Compatible with all KAPA DNA library preparation protocols
Comparable yields and size-distribution in comparison to industry-leading supplier
Readily incorporated into existing automation applications
View data on comparable performance of KAPA HyperPure Beads on DNA post-ligation yield compared to industry-leading supplier.
Figure 1. KAPA HyperPure Beads provide superior recovery performance to Supplier A in DNA workflows. (A) Recovery of unfragmented human gDNA. (B) Recovery of highly degraded and moderately degraded formalin-fixed paraffin-embedded (FFPE) human genomic DNA. Highly degraded DNA obtained from FFPE clinical research samples (n=12); moderately degraded DNA obtained from Horizon Reference FFPE DNA (n=9). For both (A) and (B), KAPA HyperPure Beads and Supplier A beads were used at a 3X ratio to clean up DNA inputs of various amounts. Recovery was measured using the Qubit Fluorometer 3 dsDNA HS Assay Kit before and after cleanup.
Figure 2. KAPA HyperPure Beads provide superior recovery performance to Supplier A in DNA workflows. (A) Recovery of unfragmented human gDNA. (B) Recovery of highly degraded and moderately degraded formalin-fixed paraffin-embedded (FFPE) human genomic DNA. Highly degraded DNA obtained from FFPE clinical research samples (n=12); moderately degraded DNA obtained from Horizon Reference FFPE DNA (n=9). For both (A) and (B), KAPA HyperPure Beads and Supplier A beads were used at a 3X ratio to clean up DNA inputs of various amounts. Recovery was measured using the Qubit Fluorometer 3 dsDNA HS Assay Kit before and after cleanup.
Figure 3. KAPA HyperPure Beads provide tunable size selection of double-stranded DNA. At each step of the KAPA HyperPrep library preparation workflow, DNA was subjected to double-sided size selection using different bead-to-sample ratios (see legend) and then analyzed for size distribution with a Bioanalyzer® 2100 High Sensitivity Kit. Each panel shows the resulting fragment size ranges compared to same-stage DNA that was not size-selected. DNA concentrations were normalized prior to loading onto the Bioanalyzer. (A) Post-fragmentation size selection of input DNA. High-quality hgDNA was mechanically fragmented with a Covaris® E220 Focused Ultrasonicator using conditions optimized to yield a mode fragment length of 250 – 400 bp, then subjected to size selection. (B) Post-amplification size selection. Libraries were amplified and final libraries were then subjected to size selection.
Figure 4. KAPA HyperPure Beads provide tunable size selection of double-stranded DNA. At each step of the KAPA HyperPrep library preparation workflow, DNA was subjected to double-sided size selection using different bead-to-sample ratios (see legend) and then analyzed for size distribution with a Bioanalyzer® 2100 High Sensitivity Kit. Each panel shows the resulting fragment size ranges compared to same-stage DNA that was not size-selected. DNA concentrations were normalized prior to loading onto the Bioanalyzer. (A) Post-fragmentation size selection of input DNA. High-quality hgDNA was mechanically fragmented with a Covaris® E220 Focused Ultrasonicator using conditions optimized to yield a mode fragment length of 250 – 400 bp, then subjected to size selection. (B) Post-amplification size selection. Libraries were amplified and final libraries were then subjected to size selection.
