Sample preparation for next-generation sequencing (NGS) entails a series of complex steps to allow for construction of DNA and RNA sequencing libraries. Successful sequencing requires high quality library construction of sufficient yield, where the workflow depends on the NGS application. This includes the following stages: input sample QC, DNA or RNA library construction and target enrichment (optional), library amplification and library quantification prior to sequencing.
Because of the number of steps involved and their inherent complexities, each step can potentially introduce errors or biases that could significantly impact the success and reliability of sequencing results. Therefore, the use of high quality reagents and optimized protocols are required to obtain superior sequencing libraries with the lowest bias, and the highest and most uniform coverage.
Roche offers a range of sample preparation reagents and high-quality enzymes engineered through our Directed Evolution Technology for every step of the NGS sample preparation workflow.