Overview

The KAPA RNA HyperPrep Kits utilize a novel chemistry that enables the combination of enzymatic steps and fewer reaction purifications, resulting in a truly streamlined solution for the preparation of high-quality RNA-seq libraries. The strand-specific workflow is flexible, supporting library construction from lower-input amounts and degraded samples and is compatible with mRNA capture and ribosomal RNA and/or globin transcript depletion.

Every KAPA RNA HyperPrep Kit includes the same core library construction chemistry with an optional upfront depletion module, making up four workflow solutions: either mRNA capture, rRNA depletion, rRNA and globin depletion or none. Roche Sequencing Solutions provides a complete library preparation solution with KAPA Pure Beads (included) and KAPA Dual-Indexed Adapters (available separately). These kits are designed to be automation-friendly on a majority of commercially available liquid handlers.

Features and Benefits of KAPA RNA HyperPrep Kits

• Reduced hands-on and overall time through fewer enzymatic and reaction cleanup steps*
• Strand-specific, sequencing-ready libraries from input RNA in ~4 hours*
• Single-day library construction, including mRNA capture and rRNA depletion with the KAPA RNA HyperPrep workflow*
• Robust performance across different sample types and input amounts*
• High throughput and consistency with an automation-friendly workflow
• Validated KAPA RNA HyperPrep Kit data analysis solutions provided through the Genialis platform