For Research Use Only. Not for use in diagnostic procedures.
KAPA Pure Beads offer a tunable and highly consistent solution for reaction purification and size selection in KAPA RNA HyperPrep and KAPA HTP/LTP next-generation sequencing (NGS) library construction workflows.
View data on comparable performance of KAPA Pure Beads on DNA and RNA post-ligation yield compared to industry-leading supplier.
Figure 3. KAPA Pure Beads provides comparable performance to Supplier ‘A’ in both DNA and RNA workflows. Libraries were prepared with the KAPA HyperPlus Kit, from 100 ng high-quality E. coli genomic DNA, fragmented at 37˚C for 30 min to target a final library size of 300 bp.
Figure 4. KAPA Pure Beads provides comparable performance to Supplier ‘A’ in both DNA and RNA workflows. Libraries were prepared with the KAPA Stranded RNA-Seq Kit with RiboErase, from 100 ng of Universal Human Reference (UHR) RNA according to standard protocol. Yields were measured with the KAPA Library Quantification Kit post ligation. Electropherograms for all libraries were generated with a Bioanalyzer 2100 High Sensitivity DNA Kit.
View data showing tunable final library size distribution using KAPA Pure Beads compared to industry-leading supplier, and data on adjustable size selection at various points in library preparation using KAPA Pure Beads.
Figure 5. Highly reproducible final library size distribution is achieved with KAPA Pure Beads. All libraries were prepared with the KAPA HyperPrep Kit, from 1 μg high-quality E. coli genomic DNA, fragmented with a Covaris E220 Focused Ultrasonicator using conditions optimized for mode fragment lengths of 350 – 450 bp. Size selection (0.6X – 0.8X) using either KAPA Pure Beads (green) or Agencourt AMPure XP (blue) was performed after library amplification (2 cycles). Electropherograms were generated with a PerkinElmer LabChip GX DNA High Sensitivity Assay.
Figure 6. Highly reproducible final library size distribution is achieved with KAPA Pure Beads. All libraries were prepared with the KAPA HyperPrep Kit, from 1 μg high-quality E. coli genomic DNA, fragmented with a Covaris E220 Focused Ultrasonicator using conditions optimized for mode fragment lengths of 350 – 450 bp. Size selection (0.6X – 0.8X) using either KAPA Pure Beads (green) or Agencourt AMPure XP (blue) was performed after library amplification (2 cycles). Electropherograms were generated with a PerkinElmer LabChip GX DNA High Sensitivity Assay.
Figure 7: Adjustable size selection at various points in library preparation. Equivalent final library size is achieved by performing size selection either immediately after genomic DNA fragmentation or after adapter-ligation (first graph). Various final library sizes are achieved by tuning size selection parameters after adapter-ligation (second graph). All libraries were prepared with the KAPA HyperPrep Kit, from 100 ng high-quality human genomic DNA, fragmented with a Covaris E220 Focused Ultrasonicator using conditions optimized for mode fragment lengths of 350 – 450 bp. Electropherograms were generated with an Agilent 2100 Bioanalyzer High Sensitivity DNA Kit.
Figure 8. Adjustable size selection at various points in library preparation. Equivalent final library size is achieved by performing size selection either immediately after genomic DNA fragmentation or after adapter-ligation (first graph). Various final library sizes are achieved by tuning size selection parameters after adapter-ligation (second graph). All libraries were prepared with the KAPA HyperPrep Kit, from 100 ng high-quality human genomic DNA, fragmented with a Covaris E220 Focused Ultrasonicator using conditions optimized for mode fragment lengths of 350 – 450 bp. Electropherograms were generated with an Agilent 2100 Bioanalyzer High Sensitivity DNA Kit.
*Data on file.
All graphic data is on file, unless otherwise noted.
KAPA HyperPure Beads may be shipped on dry ice which does not affect product performance. Upon receipt they must be stored at 2⁰C to 8⁰C.
| Kit Code |
Roche Cat. No | Description | Kit Size |
|---|---|---|---|
| KK8000 |
07983271001 |
KAPA Pure Beads (5 mL) |
5 mL |
| KK8001 |
07983280001 |
KAPA Pure Beads (30 mL) |
30 mL |
| KK8002 |
07983298001 |
KAPA Pure Beads (60 mL) |
60 mL |
