Roche introduces its target enrichment custom panels, KAPA HyperPETE Choice Panels and KAPA HyperPETE Explore Panels, for DNA or RNA. KAPA HyperPETE Choice Panels and KAPA HyperPETE Explore Panels enable custom designs up to 250Kb for DNA inputs and 50Kb for RNA inputs.
Quickly and efficiently cover your custom regions with the high-performing KAPA HyperPETE Custom Panels
Rely on Roche’s proven design and primer selection expertise to improve your desired target coverage and maximize the data return from your research. Use the HyperDesign Tool to prepare your custom design in four (4) simple steps, include an optimized MSI (Microsatellite Instability) target set into your design or consult with Roche’s expert designers team for your special design needs.
Take fewer steps to optimal performance and sequencing efficiency
Experience high performance from your very first panel design iteration as a result of Roche’s design expertise and extensive KAPA HyperPETE Workflow optimization.
Table 1 (a, b, c, d). Highly confident detection of SNVs, Indels, CNV and MSI using Horizon Discovery FFPET control and CrownBio FFPET xenograft samples. Performance was demonstrated using a KAPA HyperPETE Choice Panel (capture target of 88 Kb plus 25 Kb of the Roche preset MSI targets). True positive detection rates were 100% for SNVs, or Indels (Table 1a), CNVs (Table 1b) and MSI (Table 1c), without any false negatives. False positive rate was 0% for microsatellite stable samples (Table 1d). Final libraries were sequenced on an Illumina NextSeq™ 550 system. Total read pairs (2x150 bp) per sample ranged from 12.5M to 20.4M and data was analyzed using NAVIFY® Mutation Caller to assess the variant detection.
KAPA HyperPETE Choice RNA Panel enables confident fusion detection from RNA samples
Table 2. Fusion detection performance using tissue RNA (FFPET). All fusions (100%) were detected in the reference cell line sample at 10 ng RNA input amount. The data of the reference cell-line was used to assess fusion detection performance. Data analysis was performed using the NAVIFY® Mutation Caller.