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What is the KAPA HyperPETE Workflow?
What is the KAPA HyperPETE Workflow?
KAPA HyperPETE Workflow is the latest innovation in the Roche N
KAPA HyperPETE Workflow is the latest innovation in the Roche NGS target enrichment portfolio - specifically designed to target panels up to 250KB. The single-day workflow allows for a fast and efficient approach to accelerating discovery—in somatic oncology research and beyond. With the support of a robust and innovative secondary analysis solution, this best-in-class workflow is uniquely equipped to help you deep-dive into new discoveries.
A streamlined and automatable workflow that does not compromi
A streamlined and automatable workflow that does not compromise performance
Redefine efficiency and precision with:
Redefine efficiency and precision with:
Specialized technology can help you meet the growing needs of y
Specialized technology can help you meet the growing needs of your lab
Flexibility by design
Flexibility by design
Tomorrow’s lab, today
Tomorrow’s lab, today
Improved sensitivity & specificity for unparalleled confidence
Improved sensitivity & specificity for unparalleled confidence in results
ACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACG
ACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACG
Easier detection and wider coverage across multiple variants a
Easier detection and wider coverage across multiple variants and sample quality
KAPA HyperCap Workflow
KAPA HyperCap Workflow
250KB and beyond
250KB and beyond
With our advanced and flexible sequencing solutions, more labs
With our advanced and flexible sequencing solutions, more labs can better understand mutations in the human genome and expand the limits of discovery. Through groundbreaking research, we hope to uncover new methods for diagnosis helping to make personalized healthcare a reality.
For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.
MC--08295
MC--08295
KAPA HyperPETE Workflow
KAPA HyperPETE Workflow
Up to 250KB
Up to 250KB
2x ~30-minute hybridization
2x ~30-minute hybridization
Overnight hybridization
Overnight hybridization
The discovery is in the details
The discovery is in the details
Biotinylated Capture Probe
Biotinylated Capture Probe
Biotinylated Capture Primer, non-biotinylated Release Primer
Biotinylated Capture Primer, non-biotinylated Release Primer
Biotinylated target-specific capture primers hybridize to targe
Biotinylated target-specific capture primers hybridize to target DNA and are extended by a DNA polymerase.
1
1
PETE Workflow (4 hour)
PETE Workflow (4 hour)
Capture primer Panel
Capture primer Panel
B
B
Biotinylated Capture Extension
Biotinylated Capture Extension
2
2
B
B
S
S
A
A
Utilizing paramagnetic streptavidin beads, targeted DNA is capt
Utilizing paramagnetic streptavidin beads, targeted DNA is captured, and off-target fragments are washed away.
Streptavidin bead capture
Streptavidin bead capture
Target-specific release primers hybridize to target DNA upstrea
Target-specific release primers hybridize to target DNA upstream of capture primers.
Release primer hybridization
Release primer hybridization
3
3
B
B
S
S
A
A
Release primer panel
Release primer panel
4
4
Release primers are extended and support release of target DNA.
Release primers are extended and support release of target DNA.
Release extension
Release extension
Released target DNA is amplified and prepared for sequencing.
Released target DNA is amplified and prepared for sequencing.
PCR of enriched library
PCR of enriched library
B
B
S
S
A
A
B
B
S
S
A
A
B
B
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S
A
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