KAPA HyperPrep Kits offer a streamlined library preparation protocol that combines several enzymatic steps and eliminates bead cleanups to significantly reduce library preparation time and improve consistency. The novel, single-tube chemistry offers further improvements to library construction efficiency, particularly for challenging samples such as FFPE and cell-free DNA. KAPA HyperPrep Kits offer complete library preparation solution with KAPA Adapters and KAPA HyperPure Beads.


Features and Benefits of KAPA HyperPrep Kits

  • Robust and reproducible library construction in 2-3 hours with high conversion rates and library complexity, particularly for FFPE
  • PCR-free workflows from lower sample inputs and improved sequence coverage for workflows with PCR
  • Compatibility with a wide range of sample types and inputs, and flexibility with respect to fragment size, adapter design and library amplification
  • Qualified automation methods
  • Validated with the HyperCap Workflow

Product highlights


Improved library yields and quality

  • Higher library yields translating to greater molecular complexity
  • Higher conversion of input DNA to adapter-ligated library
  • Fewer cycles of amplification with KAPA HiFi DNA Polymerase, resulting in lower duplication rates and improved coverage


Minimal amplification bias

  • Kits with or without an amplification module
  • Lower PCR bias resulting in more uniform coverage in PCR workflows


High coverage of FFPE samples

  • Higher library yield, higher library complexity and fewer PCR cycles
  • Lower duplication rates and higher coverage


Improved performance with cell-free DNA

  • Ability to optimize adapter:insert ratios for challenging sample types, such as cell-free DNA
  • Higher library density compared to competitor kit*
  • Comparable duplication rates to vendor kits specific for low-input applications

*Data on file.
All graphic data is on file, unless otherwise noted.