KAPA HyperPlus Kits for NGS DNA Library Prep

Overview

KAPA HyperPlus Kits, our most advanced library preparation option, provide a streamlined workflow that includes fully automatable fragmentation and library preparation in a single tube. This kit is based on our DNA Hyper portfolio which offers industry-leading library construction efficiencies. This integrated solution combines enzymatic fragmentation, similar in quality to mechanical shearing, with the speed and convenience of tagmentation-based workflows.

KAPA HyperPlus Kits offer a complete library preparation solution with KAPA Adapters and KAPA HyperPure Beads (sold separately). The kits are compatible with the Illumina sequencing platform and provide qualified automation methods.

Features and Benefits of KAPA HyperPlus Kits

Fully automatable DNA library construction in 1.5-3 hours with low-bias enzymatic fragmentation
Industry-leading conversion rates and library complexity,* particularly for FFPE DNA
Now includes an additional End Repair and A-Tailing module for more sensitive applications with no change to the workflow
PCR-free workflows from lower inputs and improved sequence coverage in workflows with PCR
Compatibility with a wide range of sample types and inputs, and flexibility with respect to fragment size, adapter design and library amplification

Product highlights

Industry-leading library construction yields* with integrated workflow

Low-input samples no longer require specialist library construction workflows or reagents
PCR-free workflows possible from as little as 50 ng starting material
Both the existing and upgraded KAPA HyperPlus chemistry produce the highest post-ligation yields

Validated with the HyperCap Workflow

View data for higher post-ligation yields produced by the KAPA HyperPlus Kit compared NEBNext Ultra | | FS Kit or Covaris-sheared DNA with KAPA HyperPrep Kit.

Tunable and low-bias enzymatic fragmentation

Library insert sizes adjustable from 150–800 bp by varying fragmentation time or temperature
Robust and reproducible fragmentation across a range of GC content and DNA input amounts and sample types

See performance data showing tunability and consistency in DNA library insert sizes with samples from different species, and comparative data showing minimal fragmentation bias by KAPA HyperPlus kits compared to Nextera Rapid Capture Exome Kit as well as HyperPrep Kit with Covaris shearing.

Superior sequencing results with KAPA HyperPlus Kits

Higher post-ligation yields result in fewer amplification cycles and lower duplication rates
Detect low-frequency mutations with high confidence due to decreased sequencing artifacts and increased sensitivity with the upgraded version of the KAPA HyperPlus Kit

View comparative data showing similar duplication rates and sensitivity, with improved sequencing results using the upgraded KAPA HyperPlus Kit compared to KAPA HyperPrep Kits with Covaris shearing.

Improved coverage depth for FFPE samples compared to Covaris-sheared DNA

Significant increase in coverage depth compared to KAPA HyperPrep Kits using Covaris-sheared DNA
Lower duplication rates, more on-target reads and more bases covered compared to KAPA HyperPrep Kits using Covaris-sheared DNA
Detection of variants with high degree of correlation between libraries prepared from KAPA HyperPrep Kits using Covaris-sheared DNA versus HyperPlus Kits*

View performance data showing significantly higher coverage for FFPE samples using KAPA HyperPlus kits compared to Covaris-sheared DNA

Minimal sequence coverage bias

Lower sequence bias when compared to other enzymatic fragmentation methods
Less bias leads to more uniform sequencing coverage and reduced sequencing costs

View GC-bias plots showing superior performance using KAPA HyperPlus Kits compared to NEBNext® Ultra™ FS and QIAseq FX workflows.

Featured Webinar

NGS library preparation: Theory and practice
Part 3: A practical example—Library preparation with the KAPA HyperPlus Kit

Presenter: Dr. Maryke Appel, Roche

Watch Now