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RNA Library Preparation
| No RNA Enrichment |
mRNA Capture |
Ribosomal Depletion |
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|---|---|---|---|---|---|---|
| cDNA Library Prep Workflow |
Standard |
Streamlined |
Standard |
Streamlined |
Standard |
Streamlined |
| KAPA Product |
KAPA Stranded RNA-Seq Kit |
KAPA RNA HyperPrep Kit |
KAPA Stranded mRNA-Seq Kit |
KAPA mRNA HyperPrep Kit |
KAPA Stranded RNA-Seq Kit with RiboErase |
KAPA RNA HyperPrep Kit with RiboErase |
| Library Prep Workflow time |
6 – 8 hr |
4 hr |
8 – 10 hr |
5.5 hr |
10 – 12 hr |
6.5 hr |
| Input Amount |
10 – 400 ng into library prep |
1 – 100 ng into library prep |
100 ng – 4 μg into mRNA capture |
50 ng – 1 μg into mRNA capture |
100 ng – 1 μg into rRNA depletion |
25 ng – 1 μg into rRNA depletion |
| Sample Type |
High-quality Total RNA | High-quality Total RNA | High-quality Total RNA | |||
| Degraded or FFPE Total RNA | Degraded or FFPE Total RNA | |||||
| Enriched RNA |
||||||
| Species |
Eukaryotic (Animal, Plant, etc.) |
Eukaryotic (Animal, Plant, etc.) |
Human, Mouse, and Rat |
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| Prokaryotic (Bacterial, etc.) |
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Differentiating Applications |
Targeted RNA-Seq |
mRNA-Seq |
Non-coding RNA | |||
| Whole Transcriptome |
Whole Transcriptome |
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| Shared Applications |
Gene Expression Analysis; Detection of Gene Fusions, Isoforms, and other Structural Variants; Novel Transcript Identification; SNV Discovery |
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| Strand-specific |
Yes | |||||
| Automation friendly |
Yes | |||||
KAPA RNA HyperPrep Kits
As the latest generation of library preparation kits, KAPA RNA HyperPrep Kits utilize a novel chemistry and combines enzymatic steps to achieve fewer reaction purifications and yield high-quality RNA-Seq libraries in a single day.
Learn moreKAPA mRNA HyperPrep Kits
KAPA mRNA HyperPrep Kits enrich for polyadenylated species and provide a focused view of the coding regions. Kits provide high coverage uniformity and minimal PCR duplicates.
Learn moreKAPA RNA HyperPrep Kits with RiboErase
KAPA RNA HyperPrep Kits with RiboErase provide consistent and effective rRNA depletion and reduce the number of reads wasted due to alignment to rRNA loci. Kits provide high coverage uniformity and yield high-quality libraries with degraded inputs, including FFPE samples.
Learn moreKAPA Stranded RNA-Seq Kits
As the first generation RNA library preparation kits, the KAPA Stranded RNA-Seq Kits combine a “with-bead” protocol with the robustness of KAPA HiFi DNA Polymerase to yield high-quality RNA libraries.
Learn moreKAPA Stranded mRNA-Seq Kits
KAPA Stranded mRNA-Seq Kits make use of KAPA mRNA Capture Beads prior to library preparation and provide a focused sequencing of protein-coding transcripts.
Learn moreKAPA Stranded RNA-Seq Kits with RiboErase
KAPA Stranded RNA-Seq Kits with RiboErase provide consistent and effective rRNA depletion yielding high coverage of transcripts of interest, including noncoding and precursor transcripts. Compatible with degraded inputs, including FFPE samples.
Learn more