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KAPA Adapters
KAPA Unique Dual-Indexed (UDI) Adapters and KAPA Dual-Indexed (DI) Adapters
Full-length dual-indexed (DI) and unique dual-indexed (UDI) KAPA Adapters support a wide variety of applications, but are particularly suited for PCR-free workflows as sequencing barcodes are added during the ligation step of library construction.
- KAPA Unique Dual-Indexed (UDI) Adapters contain non-redundant sequencing barcode combinations, designed to mitigate the impact of index misassignment on Illumina instruments with patterned flow cells
- KAPA Dual-Indexed (DI) Adapters employ a combinatorial dual indexing strategy
KAPA Universal Adapters and KAPA UDI Primer Mixes
Coming late 2019.
| KAPA Unique Dual-Indexed Adapters | KAPA Dual-Indexed Adapters | |
|---|---|---|
| Recommended for instruments with non-patterned flow cells (e.g. MiSeq 3000, NextSeq 4000, HiSeq 2000, HiSeq 2500) |
Yes | Yes |
| Recommended for instruments with patterned flow cells (e.g. HiSeq 3000, HiSeq 4000, HiSeq X, Novaseq) |
Yes | No |
| Designed to mitigate index assignment |
Yes | No |
| Number of full-length, dual-indexed adapters |
96 | 96 |
| Nature of dual sequencing indexes |
Unique | Combinational |
| Number of 8-nt non-reduntant p5 barcodes | 96 | 8 |
| Number of 8-nt non-reduntant p7 barcodes | 96 | 12 |
| Barcodes identical to those used in adapters supplied by illumnia | No (a) | Yes |
| Compatible with KAPA HyperPrep, KAPA HyperPlus, KAPA RNA HyperPrep and HyperCap workflows |
Yes | Yes |
| Compatible with Roche Universal Blocking Ollgos |
Yes | Yes |
| Compatible with methyl-seq applications (b) |
No | No |
| Adapter formulation | Ready-to-use, 15 mM solutions; 20mL per adapter (c) | |
| Kit configuration | Hard-shell plate with automation-friendly labelling and overfills and replacements seats (d) |
|
| KAPA Adapter Dilution Buffer included | Yes (25 mL per kit) | |
(a) The set of 192 barcode sequences used in KAPA UDI Adapters is exclusive to Roche.
(b) SeqCap adapters are methylated and may be used in combination of KAPA library construction kits in bisulfite sequencing workflows that are that require the ligation of methylated adapters during library construction.
(c) This is sufficient for four library preps with the KAPA HyperPrep or KAPA HyperPlus Kit if no adapter dilution is required. Generous overfill supports use on automated liquid handling system.
(d) KAPA Adapter plates are shipped with peelable seals. Replacement seals for KAPA UDI Adapter Kits are peelable and pierceable whereas replacement seals for KAPA DI Adapters Kits are peelable only.
