Library preparation for next-generation sequencing (NGS) often involves an amplification step for obtaining fragments enriched for adapter ligated ends and for increasing the library size, both of which are essential for effective detection of sequences during a sequencing run. However, PCR can often introduce bias for sample composition, leading to potential underrepresentation of these fragments in a sequencing read.
Roche Sequencing Solutions offers KAPA Library Amplification Kits, which contain KAPA HiFi DNA polymerase, a novel enzyme engineered through our directed evolution technology, for ultra-high fidelity and robustness. KAPA HiFi improves the overall sequencing quality with low amplification bias, more uniform coverage of difficult regions, lower duplication rates compared to other options, and amplifies NGS libraries with industry-leading fidelity.*