Simplify your NGS sample preparation workflows with the AVENIO Edge System, a fully-automated IVD liquid handler for an end-to-end library preparation, target enrichment, quantification, normalization and pooling steps of an NGS workflow.
To experience the AVENIO Edge Liquid Handler difference for NGS Sample preparation
NGS library prep workflows are labor-intensive and time-consuming—especially if they include target enrichment (with oncology panels, for example). As a result, many genomics laboratories rely on automated library preparation to streamline processes, reduce user-to-user variability, and improve reproducibility.
However, many of the automated liquid handlers on the market are cumbersome to program, set up, and run. Now, with the AVENIO Edge Liquid Handling System, labs that have no prior experience with NGS or automation can easily run complex library prep workflows—freeing up hours of time for other projects and reducing errors that can occur with manual methods.
AVENIO Tumor Tissue CGP Automated Kit enables laboratories to implement an end-to-end comprehensive genomic profiling (CGP) workflow without the daunting hurdles that could hinder discoveries. This end-to-end CGP workflow features a 335-gene panel aligned with the FoundationOne® CDx panel design, automated by the AVENIO Edge Liquid Handing System, and driven by the proven bioinformatics of the FoundationOne® Analysis Platform. With the expertise and proven technology of Roche and Foundation Medicine, labs can finally perform CGP with efficiency and renewed confidence.
Join Dr. Christian Betz, Ph.D., Head of Molecular Genetics at Bioscientia (part of Sonic Healthcare), for this recap of his ESHG 2024 presentation. In less than five minutes (video on the left), Dr. Betz details how his lab established a high-throughput, cost-effective whole-exome sequencing (WES) workflow using an approach that has become the backbone for all next-generation sequencing (NGS) analysis at Bioscientia, one of Germany's largest human genetics laboratories.
Queen's University Belfast shares how the AVENIO Edge System got their ACTIONED Project on track.
"Using the Avenio Edge System we can free up team members to work in different parts of the project, or even take [on] new projects that bring additional value. The AVENIO Edge System has played a vital role in making sure that we can keep our TAT in check." Prof. David Gonzalez de Castro, PhD FRCPath
"The amount of the ctDNA that we have for each sample is very small. We therefore have only one opportunity to run the ctDNA on a library prep. With the AVENIO Edge we have the confidence and reliability that we can run ctDNA samples and [know] it will work." Peter Stewart, PhD
Watch this ~2 min video to learn about the capabilities and specifications of the AVENIO Edge Liquid Handler for NGS library prep and target enrichment. The AVENIO Edge provides an end-to-end library prep workflow, including cleanup steps, quantification, normalization, and pooling.
Watch this video to learn how the AVENIO Edge IVD Liquid Handling System helps make it easier for molecular pathology labs to NGS libraries for applications such as cancer research, exome sequencing, and beyond.
To experience the AVENIO Edge Liquid Handler difference for NGS Sample preparation
Here is a growing menu of workflows currently available on the system:
Here is a growing menu of workflows currently available on the system:
| Workflow | Maximum samples (per run) | Estimated setup time (minutes) |
|---|---|---|
| AVENIO Edge HyperPlus or AVENIO HyperPlus DNA library preparation* | 48 | 20 |
| AVENIO Edge Target Enrichment with KAPA HyperExome or custom panels** | 48 | 20 |
| AVENIO Edge HyperCap DNA library preparation and target enrichment* | 24 | 20 |
| Input DNA quantification, normalization and buffer exchange | 96 | 15 |
| Post-capture pooling and quantification | 48 | 15 |
| *These workflows use UDI primers and universal UMI or non-UMI adapters. **The input is indexed DNA or cDNA libraries |
||
9. Cooling unit
10. Tube racks
11. Piercing tool
12. Reagent mini racks
13. Reagent drawer
14. P1000, P200, P50 Filtered tips
15. Quantification plate station
16. Consumable drawer
17. Gripper fingers exchange station
18. Quantification module
19. 2D Barcode reader
20. Processing plates
21. Processing plate lids
Roche’s NGS Automation Support Team, in collaboration with non-Roche liquid handling vendors, creates menus of automated methods for KAPA library prep reagents. Let’s talk about the next steps to get you up and running with automating your NGS library prep protocols. Visit support for non-Roche liquid handlers to learn more.
AVENIO Edge reagents and workflows are for Research Use Only. They are not intended for diagnostic applications.
This ordering guide covers reagents and plastic consumables. Please order the probes or panels separately. The word “run” on the AVENIO Edge System refers to two capabilities:
1. A full end-to-end workflow for library prep and target enrichment. This may include input control, quantification and post-capture pooling. This often consists of multiple protocols executed sequentially on the System, with user touch points at the start and finish of each protocol.
2. The end-to-end execution of a single protocol, with two user touch points for the beginning (loading) and end (unloading) of the protocol.
Depending on the number of quantifications used on each sample throughout the workflow (up to three per workflow), the second definition of “run” shall apply.
Check your local service package offering.
| Material ID Number | Description | Details |
|---|---|---|
| 09283234001 | AVENIO Edge Liquid Handling System | Required |
| 09539913001 | FortiGate 60F Firewall | Optional |
| 10169516001 | Unified Gateway (for remote access) - US version* | Optional |
| 10001224001 | AVENIO Designer Software | Required for User-Defined Workflows |
AVENIO Edge System reagents are fully barcoded, ready-to-use and modular to enable a variety of workflows. The reagent kits contain barcoded reagent mini racks, primer plates (96-well plates), troughs and/or tubes depending on the reagent volume, storage or other parameters. Each reagent kit contains enough reagents for three runs. The protocols automate a variety of workflow steps from sample input control, library preparation, target enrichment, quantification, normalization and pooling and more, all on deck. Modular design of the AVENIO Edge System empowers you to start and stop at your desired step in the workflow with an ability to select your desired batch sizes from 1-48 samples (in 8 samples per batch multiples). Consult your Roche representative for more details.
AVENIO Edge System reagents are fully barcoded, ready-to-use and modular to enable a variety of workflows. The reagent kits contain barcoded reagent mini racks, primer plates (96-well plates), troughs and/or tubes depending on the reagent volume, storage or other parameters. Each reagent kit contains enough reagents for three runs. The protocols automate a variety of workflow steps from sample input control, library preparation, target enrichment, quantification, normalization and pooling and more, all on deck. Modular design of the AVENIO Edge System empowers you to start and stop at your desired step in the workflow with an ability to select your desired batch sizes from 1-48 samples (in 8 samples per batch multiples). Consult your Roche representative for more details.
| Material ID Number | Description | Max Reactions Per Run** |
Max Reactions Per Kit |
Shipping Category |
Storage |
|---|---|---|---|---|---|
09200851001 |
AVENIO Edge Quantification Kit*** | up to 48 | 144 | >0°C and ≤+35°C | RF |
| 09200878001 | AVENIO Edge DNA HyperPlus Kit | 24 | 72 | >-20°C and ≤+25°C | FZ |
| 09200894001 | AVENIO Edge DNA HyperPrep Kit | 24 | 72 | >-20°C and ≤+25°C | FZ |
| 09200908001 | AVENIO Edge Target Enrichment, A | 8 | 24 | >-20°C and ≤+25°C | FZ |
| 09200916001 | AVENIO Edge Target Enrichment, A | 24 | 72 | >-20°C and ≤+25°C | FZ |
| 09200924001 | AVENIO Edge Target Enrichment, B | 24 | 72 | >0°C and ≤+25°C | RF |
| 09200959001 | AVENIO Edge UDI Primer Mixes | 1-96* | 96* | >0°C and ≤+25°C | RF |
| 10244844001 | AVENIO Edge UDI Adapter Kit (15uM) | 1-96* | 96* | >-20°C and ≤+25°C | FZ |
| 09200975001 | AVENIO Edge HyperPlex UMI Adapter | 8 | 24 | <-15°C & with dry ice | FZ |
| 09200983001 | AVENIO Edge HyperPlex UMI Adapter | 24 | 72 | <-15°C & with dry ice | FZ |
| 09200991001 | AVENIO Edge HyperPlex Adapters | 8 | 24 | <-15°C & with dry ice | FZ |
| 09201009001 | AVENIO Edge HyperPlex Adapters | 24 | 72 | <-15°C & with dry ice | FZ |
| 09314512001 | AVENIO Edge Solution A*** | up to 48 | 288 | >0°C and ≤+35°C | RF |
| Material ID Number | Description | Units Per Box/Carton | Units Per Pack/Rack |
|---|---|---|---|
| 09336354001 | AVENIO Edge Pipette Tip, 1000 μL | 2304 per box/96 per pack | 96 |
| 09336346001 | AVENIO Edge Pipette Tip, 200 μL | 2304 per box/96 per pack | 96 |
| 09336320001 | AVENIO Edge Pipette Tip, 50 μL | 2304 per box/96 per pack | 96 |
| 09056351001 | AVENIO Edge Tip Park | Required for User-Defined Workflows | N/A |
| 08994609001 | AVENIO Edge Processing Plate | 16 | 10 |
| 09031022001 | AVENIO Edge Processing Plate Lid | 50 | 10 |
| 09031049001 | AVENIO Edge Liquid Waste Plate | 50 | 5 |
| 09030956001 | AVENIO Edge Quantification Plate | 60 | 10 |
| 09164081001 | AVENIO Edge Tube, Panel 1 | 40 | N/A |
| 09164090001 | AVENIO Edge Tube, Panel 2 | 100 | N/A |
| 09164103001 | AVENIO Edge Tube, Panel 3 | 100 | N/A |
| 09164111001 | AVENIO Edge Tube, Panel 4 | 100 | N/A |
| 09313630001 | AVENIO Edge Pooling Tube | 100 | N/A |
The virtual kits provide you with all the reagent kits required to run 1-3 runs of end-to-end library preparation, target enrichment and quantification on deck. The virtual kits assume one quantification step per run. Should you decide to quantify your samples prior to library preparation and/or quantify your samples before pooling, you may have to order additional reagent kits separately.
Kit 1 (24 samples x 3 runs): 09496424001 AVENIO Edge HyperPrep with UMI, 24 reactions LP+TE*
| Material ID Number | Description |
|---|---|
| 09200851001 | AVENIO Edge Quantification Kit |
| 09200894001 | AVENIO Edge HyperPrep Kit |
| 09200916001 | AVENIO Edge Target Enrichment, A |
| 09200924001 | AVENIO Edge Target Enrichment, B |
| 09200959001 | AVENIO Edge UDI Primer Mixes |
| 09200967001 | AVENIO Edge HyperPure Beads |
| 09200983001 | AVENIO Edge HyperPlex UMI Adapters |
| 09314512001 | AVENIO Edge Solution A |
Kit 2 (24 samples x 3 runs): 09496432001 AVENIO Edge HyperPrep without UMI, 24 reactions, LP+TE*
| Material ID Number | Description |
|---|---|
| 09200851001 | AVENIO Edge Quantification Kit |
| 09200894001 | AVENIO Edge HyperPrep Kit |
| 09200916001 | AVENIO Edge Target Enrichment, A |
| 09200924001 | AVENIO Edge Target Enrichment, B |
| 09200959001 | AVENIO Edge UDI Primer Mixes |
| 09200967001 | AVENIO Edge HyperPure Beads |
| 09201009001 | AVENIO Edge HyperPlex UMI Adapters |
| 09314512001 | AVENIO Edge Solution A |
Kit 3 (24 samples x 3 runs): 09496459001 AVENIO Edge HyperPlus with UMI, 24 reactions, LP+TE*
| Material ID Number | Description |
|---|---|
| 09200851001 | AVENIO Edge Quantification Kit |
| 09200878001 | AVENIO Edge HyperPrep Kit |
| 09200916001 | AVENIO Edge Target Enrichment, A |
| 09200924001 | AVENIO Edge Target Enrichment, B |
| 09200959001 | AVENIO Edge UDI Primer Mixes |
| 09200967001 | AVENIO Edge HyperPure Beads |
| 09200983001 | AVENIO Edge HyperPlex UMI Adapters |
| 09314512001 | AVENIO Edge Solution A |
Kit 4 (24 samples x 3 runs): 09496467001 AVENIO Edge HyperPlus without UMI, 24 reactions, LP+TE*
| Material ID Number | Description |
|---|---|
| 09200851001 | AVENIO Edge Quantification Kit |
| 09200878001 | AVENIO Edge HyperPrep Kit |
| 09200916001 | AVENIO Edge Target Enrichment, A |
| 09200924001 | AVENIO Edge Target Enrichment, B |
| 09200959001 | AVENIO Edge UDI Primer Mixes |
| 09200967001 | AVENIO Edge HyperPure Beads |
| 09201009001 | AVENIO Edge HyperPlex UMI Adapters |
| 09314512001 | AVENIO Edge Solution A |
To place an order or ask a question
AVENIO Edge reagents and workflows are for Research Use Only. They are not intended for diagnostic applications.
