A major challenge in Next-Generation Sequencing (NGS) is the ability to process low-input samples of variable quality with predictable success rates in converting the available DNA into sequenceable molecules with minimal loss and bias.
Being able to determine the probability of sequencing success prior to library construction reduces repeats, sample processing time and cost. Commonly used sample QC methods based on spectrophotometry or fluorometry are typically insufficient to predict the yield and quality of libraries prepared from degraded DNA samples.
Roche Sequencing Solutions offers a qPCR-based qualification and input assessment kit for use with human genomic FFPET DNA samples in a single assay and an easy-to-automate workflow.